Lab 13 Biochem Part 1

What effect did high and low temperatures have on enzyme activity? Why?

An enzyme requires a Zn2+ ion in order to function correctly. In this case, the Zn2+ is a(n) a. apoenzyme b. coenzyme c. cofactor d. subunit 24. A substance that can bind to an enzyme at a location other than the active site and increase or decrease the activity of that enzyme is called a(n) a. subunit b. proenzyme c. modulator d. feedback inhibitor

Find an example of an enzyme. a) What is the chemical reaction that is catalyzed by the enzyme? b) Why is this enzyme/chemical reaction important to the organisms in which it occurs?

1. Describe enthalpy with respect to free energy change. 2. Describe the term endergonic with respect to a system's free energy change. 3. Distinguish between free energy of a reaction and activation energy for that same reaction. 4. What is an enzyme mechanism?

1. Which of the following DOES NOT describes enzymes? A. A specific enzyme can react with different substrates B. Consumed during the process of metabolism of biomolecules C. Found in both biological and inorganic reactions in the body D. Usually proteins that control rate of reactions in biological compounds 2. How does a catalyst increase the rate of a chemical reaction? A. changing the concentration of reactants B. decreasing the heat of reaction C. increasing kinetic energy D. providing an alternate reaction mechanism

Enzymes function most efficiently at the temperature of a typical cell, which is 37 degrees Celsius. Increases or decreases in temperature can significantly lower the reaction rate. What does this suggest about the importance of temperature-regulating mechanisms in organisms? How does this translate to the development of enzyme assays for clinical laboratory testing equipment? Explain.

the conversion of the amino acid phenylalanine into non-harmful tyrosine is mutated and doesn't work as well (or at all). Phenylalanine will still break down into tyrosine by itself, ol because this is a favorable reaction. Why is the non-functional enzyme a problem? Explain in a few sentences. 3. The disease Phenylketonuria (or PKU) occurs when the enzyme that helps to catalyze O p HOo (pn) O pe oHe avl lls sew.i Snde (a)H noitas on ol woisd sidst nos stolgno ta T b e sd linw ps fertt sro.nalom ni encbeono mundiups sis novi 2noitetnono uTeeg os s oulev THI utinogmet cseo.o 8880 4. Consider the following reaction, which is exothermic: C2H4 (g) + Br2 (g) → C2H¾Br2 (g) ΔΗ< Predict the effect of each of the following perturbations (shift right/products, shift left/reactants, or no effect) using Le Chatelier's principle a. The reaction temperature is increased: b. The reaction volume is decreased: C. More bromine gas is added: d. The reaction is done in the presence of helium: e. C2H4BR2…

A. Match the following Enzymes and Substrates: Enzyme III. Substrates a. Sucrase 1. Maltose b. Amylase 2. Amylose C. Lactase 3. Sucrose d. Maltase 4. Arginine e. Arginase 5. Lactose

7. An organic molecule needed to make an enzyme function correctly is called a(n) a. subunit b. cofactor C. coenzyme d. apoenzyme

Find an example of an enzyme that was NOT discussed in the book or video lectures.  Answer the following questions about the enzyme that you chose. a)  What is the chemical reaction that is catalyzed by the enzyme? b)  Why is this enzyme/chemical reaction important to the organisms in which it occurs? Please post your source (include a link to the website you read your information)

A Moving to another question will save this response. Question 23 The type of enzyme that catalyzes a reduction is known as a(n). A Moving to another question will save this response.

Please explain why this anwser was wrong for question 1 and what the errors made were. If possible also please explain how you can get the correct anwser for this problem.

Chemistry

explain why the rate of reaction initially increases with temp?

True or False? 6. Food taken in can eventually be broken down into simpler forms of metabolites to provide energy for organisms through the process of anabolism.    Write down your explanations.

13. A specific enzyme will usually only catalyze one particular reacton. True  False

The substance where the enzyme reacts with? A. Substrate B. Inhibitor C. A & B D. Activator E. Product

1. Which of the following statements is TRUE regarding pasteurization? a. The sole objective of pasteurization is to sterilize the food. b. Pasteurization is carried out at higher pressures than sterilization of non-food items. c. Generally the lower the temperature, the shorter the time also. d. Pasteurization takes into account that many foods are more sensitive to heat than the contaminating pathogens. e. Pasteurization can be carried out at room temperature

NOTE: no need for explanation, just answer it with correct letters.

Pleasee i badly need help. ASAP. Explain in 5-6 sentences. 3. Why do you think keeping food in the refrigerator prevents food spoilage.

Now that we have worked through what happens to the energy of the system when a substrate binds to an enzyme, please draw how the energy diagram (on the template below) changes as a result from binding to the enzyme. Explain your drawing, referencing forces, energy, and interactions. potential energy reactants reaction coordinate products