Pre-Lab Submission #5 Part 2
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State College of Florida, Manatee-Sarasota *
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Course
2045L
Subject
Chemistry
Date
Dec 6, 2023
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Project 2: Food Dyes Analysis of Commercial Products Part 2
CHM 2045L
Section 905
October 24, 2023
Connor Whaley, Tag Woodard, Taabish Ajaney, Nish Patel
Objective:
We will be using commercial food products that contain the dyes we selected in
part 1 of this lab. Using these products, we will be testing the absorbance of the samples. Also,
we will be looking for the concentration of dye in each sample.
Materials:
* Scientific Calculator
* Laboratory notebook and pen for recording data
* Safety goggles
* Laboratory coat
* Laboratory Gloves
* 1 mL Graduated pipettes
* 10 mL Volumetric Flask
* 250 mL Erlenmeyer Flask
* Cuvette
* Ocean Optics Spectrophotometer Instrument
*10 mL Pipette pumps
*50 mL Beaker
*Commercial food product containing Yellow #6
*Commercial food product containing Yellow #5
Connor Whaley
Chemicals/Hazards:
Chemical
Name:
Chemical
Formula:
Mol
ecul
ar
For
mul
a
Wei
ght:
Physical
Appearance:
Hazards:
Additional
Information:
Water
H
2
O
18.0
152
8
g/m
ol
Transparent
Liquid/Tinted
Blue
Not
Classified as
Hazardous
Incompatible
with metallic
sodium
Yellow 5
C16H9N4Na3O9S2
534.
3
g/m
ol
Bright,
lemon yellow
color
May cause
reaction in
the skin
Rinse eyes if
in eye contact
Yellow 6
C16H10N2Na2O7S2
452.
37
g/m
ol
Yellow liquid
Can harm
skin or eyes
if in contact
May cause
allergic skin
reaction
Other Hazards: Slips, Falls, Broken Glassware
Nish Patel
Methods & Procedures:
1. Yellow #5 and #6
2. Powerade, Orange. Yellow #5 and #6
3. Yellow #5: 430nm, Yellow #6: 429nm
4. From 0.1 to 1
Taabish Ajaney
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Arkansas Tec x
O Mail - Jackson Illingworth - Outlo
2 Content
https://bblearn.atu.edu/ultra/courses/ 32419 1/cl/outline
* Question Completion Status:
QUESTION 26
A student uses her absorbance data on dilutions of a dye to create the graph below.
0.60
0.50
0,40
0.30
0.20
0.10
0.020
0.040
0.060
0.080
0.10
Concentration (M)
If the absorbance of the unknown solution is 0.275, what is the concentration of the solution?
O a. 0.075 M
O b.2.75 x 10-3 M
O c. 0.050 M
O d. 0.025 M
QUESTION 27
Why is it generally preferable to use absorbance as a measure of sample absorption rather than % transmittance?
O a. absorbance is proportional to the concentration of the analyte, whereas %T is not
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7.
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D. Photomultiplier tubes
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0.510
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0.621
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1.40
Beer's Law Plot
1.20
1.00
0.80
0.60
0.40
0.20
0.00
0
10
20
30
40
50
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D
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M
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Part 3: Q9 Average absorbance
Flask 8
Flask 9
Flask 10
Flask 11
Part 3: Q9
Flask 8
Flask 9
Flask 10
Flask 11
Part 3: Q10
Flask 8
Flask 9
Flask 10
Flask 11
Q
Part 3
0.146
[Fe] -0.002mol/L
0.166
volume 5.00mL
0.215
0.294
[FESCN mol/L)
✪
4.59* 10
5.18*10*
6.64 x 10
9.08*10*
[Fe(mol/L)
5.00 x 10
5.00*10*
Y=3362x -0.0083 R¹ 0.9993
5.00 x 10
5.00*10*
(Amounts of flask 8 (9 - 11's values are in the spreadsheet)
1:1 Ratio so,
Molarity of Fe(NO3))3 = 0.2M
Volume of Fe(NO3))3 = 5.00mL
71%
Moles of Fe³= Molarity x volume(L)
Moles of Fe = 0.2M × 0.005L = 1× 10-³mole
68%
[SCN ](mol/
4 x 10-4
5 x 10-4
7*10*
1*10*
الخا
chem2.png
Concentration of Fe³
9,
X =
mole of Fe³1x10³ mole
Volume
0.0054
Ⓒ
8, Average absorbance for solution 8 (same for 9 - 11)
Average absorbance for flask 8 = sum of all absorbances / number of readings
(0.145 +0.147 +0.148)
3
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IV. Chemical interference occurs when a component of the sample emits light at or close to the same frequency as the analyte.
V. Releasing agents will preferentially react with the analyte, protecting the analyte from components of the sample that will ionize the analyte.
O A. II, IV, and V
B. I, II, and IV
OC.I, II, and IV
O D.I, II, and III
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