Ex12Act1HaleyAjeel

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Florida State College at Jacksonville *

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2086C

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Chemistry

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Dec 6, 2023

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11/18/23, 2:38 PM PhysioEx Exercise 12 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex12/act1/ 1/4 PhysioEx Lab Report Exercise 12: Serological Testing Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia Name: Haley Ajeel Date: 18 November 2023 Session ID: session-933d4e0d-ea78-4099-3c70-bbf767c2b4e9 Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Experiment Results Predict Questions No Predict Questions Stop & Think Questions Which of the following has an epitope? You correctly answered: an antigen. 1 Chlamydia trachomatis is You correctly answered: a bacterium that reproduces inside its host cell. 2 The infectious form of Chlamydia that is spread from person to person is the You correctly answered: elementary body that stains green in this activity. 3 The goal of the direct fluorescent antibody test is to test for the presence of You correctly answered: antigen. 4 The source of the patient samples is You correctly answered: epithelial scrapings. 1 What is the name for the specific part of the antigen that the antibody binds to? You correctly answered: epitope. 2 Drag the first slide (patient A) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies. 3
11/18/23, 2:38 PM PhysioEx Exercise 12 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex12/act1/ 2/4 Experiment Data Sample Number of Elementary Bodies Chlamydia Result Patient A 0 - Patient B 16 + Patient C 1 + Positive Control 19 + Negative Control 0 - Post-lab Quiz Results You scored 100% by answering 5 out of 5 questions correctly. You answered: 0 elementary bodies. Drag the next slide (patient B) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies. You answered: 16 elementary bodies. 4 Drag the next slide (patient C) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies. You answered: 1 elementary bodies. 5 Drag the next slide (positive control) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies. You answered: 19 elementary bodies. 6 Drag the last slide (negative control) to the fluorescent microscope. Count the number of elementary bodies you see through the microscope (recall that elementary bodies stain green), enter the number of elementary bodies. You answered: 0 elementary bodies. 7
11/18/23, 2:38 PM PhysioEx Exercise 12 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex12/act1/ 3/4 Review Sheet Results In the serology test just performed, the fluorescence is found on the You correctly answered: antibody. 1 What would most likely happen if you forgot to fix the samples to the slide? You correctly answered: They would all test negative because the washing steps would remove the samples. 2 Which sample showed some residual nonspecific binding after the washing steps? You correctly answered: patient C. 3 Which patient samples contained the Chlamydia -specific antigen? You correctly answered: patient B. 4 How is chlamydia disease transmission prevented? You correctly answered: abstinence or faithful, mutual monogamy. 5 Describe the importance of the washing steps in the direct antibody fluorescence test. Your answer: The washing steps are extremely important because they remove any nonspecific binding of secondary antibodies. 1 Explain where the epitope (antigenic determinant) is located. Your answer: Epitope (antigenic determinant) is located at a small site on an antigen where a complementary antibody may specifically bind. Normally, this is one to six monosaccharides or five to eight amino acid residues on the surface of the antigen. 2 Describe how a positive result is detected in this serological test. Your answer: A positive result is detected in this serological test when fluorescently labeled antibodies bind to the epitope regions found on the elementary bodies (turning green) when present. 3 How would the results be affected if a negative control gave a positive result? Your answer: 4
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11/18/23, 2:38 PM PhysioEx Exercise 12 Activity 1 https://media.pearsoncmg.com/bc/bc_0media_ap/physioex/10/ex12/act1/ 4/4 If a negative control gave a positive result, this would tell me that there may have been contamination between the specimen. It would be best to start over with new specimen to ensure any contamination is disposed of in order to provide accurate results.