580360 Isolation of Casein Q
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Isolation of Casein
Jerret Jarvis
November 10, 2023
1
Data
Activity 1
Data Table 1
Dry Milk Sample
Optional Milk Sample
Mass of Beaker
1.77
1.77
Mass of Beaker + Milk
10.00
11.00
Mass of Milk
8.23
9.33
pH of Milk pre-acid
7 pH
8 pH
pH of Milk post-acid
3 pH
4 pH
Mass of Filter Paper
1.23
1.23
Mass of Filter Paper +
Casein
1.87
2.73
Mass of Casein
22.7
29.2
Percentage of Casein
in Milk
18.4%
23.7%
1.
What purpose was served by filtering the milk sample after the addition of
acid?
~To sperate the fat protein from the milk.
2.
When weighing the protein at the end of the activity, what major
assumption is made about the product? Why might this impact the
calculated percentage of casein in the sample?
~ It is to show how much of the protein is in milk compared to fat and
water.
3.
If a base, such as sodium hydroxide (NaOH) were added to milk, would the
protein precipitate? Why or why not?
~I don’t think it would precipitate, I think just more minerals or fat would
be added to the solution.
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pH = 4.389
pH = 4.874
=
pH = 5.339
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09. Two samples of the same soil is subjected to pH analysis using a pH meter, the first is analysed using 1:1 soil:water ratio and the other with 1:1 soil:KCl ratio, what would be the most probable result?
A. Soil analused using 1:1 soil:KCl ratio will register a higher pH compared to that of soil analysed using 1:1 soil water ratio
B. Soil analysed using 1:1 soil:KCl will register a lower pH compared to that of soil analysed using 1:1 soil:water ratio
C. Soil analysed using 1:1 soil:water ratio will register a lower pH compared to that of soil analysed using 1:1 soil:KCl ratio
D. Soil analysed using 1:1 soil:water ratio will have the same pH with that of soil analysed using 1:1 soil:KCl ratio
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Can you please show me the computation or how to compute? Thank you.
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the question states that the Ka value was 3.0 x 10-8 but in step two to calculate the value of pKa you calculated using the -log of 30.0 x 10-8 why did you use that number instead?
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Data in first picture and questjons in second
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Complete the following table "predicted pH." Please show all work on a separate sheet of paper
and attach. Note that the pK, of H₂PO4 is 7.2.
Actual pH
Predicted pH
Original After 45 µL of 5
M NaOH
solution
4.30
6.05
After 205 μL more
of 5M NaOH
6.90
After 205 μL more
of 5M NaOH
11.01
After 45 μL more
of 5 M NaOH
11.29
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A weak base has KB = 3.26 x 10-2. What is the pKB of this base?
Report your answer to at least 1 decimal place.
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Electrical
Conductivity of
solution
lons released in
solution
Solution
pH
Taste
1
Very high
8.0
bitter
ОН-
2
Very high
14.0
bitter
ОН -
none
7.0
none
both
4
Very high
2.0
H+
sour
Based on the experimental data collected by a student above, which solution is
acidic? Give at least 2 reasons to support your answer.
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[OH-¹] =
[H3O+¹] =
pOH = -log [OH-¹] =
a. acetic b. hydrochloric
g. sulfurous
f. sulfuric
c. phosphoric d. perchloric
h. hydrosulfuric i. OH-1 j. H30+1 k. SO4²
0. H₂PO3-1 p. CO3² q. HCO3-¹
e. hydrofluoric
1. Mg+2
-2
-2
n. HPO3-²
-2
r. H₂S
t. S-²
u. 0.2500
v. 0.1000
S. HS-1
y. 3.0000
w. 0.02500
z. 1x 10-14
x. 1.000 x 10-3
dd. 2
aa. 2.6990
ee. 1
ff. 2.000 x 10-³
gg. 5.000 x 10-12
mm. conjugate acid
bb. 1.000 x 10-11
hh. 11.3010
oo. base
CC. 11.0000
ii. 0.01250
pp. conjugate base
jj. 0.22100
kk. 0.05656
m. Cl-1
II. acid
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I. 1.0 x 10-3 M H3PO4
pka3 = 12.375]
II. 1.0 x 10-3 M H2SO3
III. 1.0 x 108 M H2SO3
IV. 1.0 x 10-8 M H2C2O4
[pKa₁ = 2.148; pKa2 = 7.198;
[pKa₁ = 1.857; pKa2 = 7.172]
[pKa₁ = 1.857; pKa2 = 7.172]
[pKa₁ = 1.250; pKa2 = 4.266]
a. Il only
b. II and IV
c. IV only
d. I only
e. I, II, III, and IV
f. Ill only
g. I, II, and IV
The pH of 0.050 M K₂HPO4 is
Use the pKa values given in Question 9.
The pH of 0.050 M K3PO4 is
Use the information given in Question 9 for H3PO4.
Hint: A quadratic equation is involved.
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В
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50.00
50.00
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35.10
36.94
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0.00
0.10
0.20
Net Volume NAOH used (ml)
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H2O used is 138 moles
2. Check the pH of this solution and record.
3. A5 M NAOH solution has been provided.
4. Add 45 µl of 5 M NaOH into the 50 mM H2PO, solution. Check the pH and record.
5. Add 205 µl of 5M NaOH into the 50 mM H2PO4 solution. Check the pH and record.
6. Add 205 µL more of 5M NaOH into the 50 mM H2PO, solution. Check the pH and record.
7. Add 45 µl of 5 M NaOH into the 50 mM H2PO, solution. Check the pH and record.
Complete the following table "predicted pH." Please show all work on a separate sheet of paper
and attach. Note that the pK, of H2PO, is 7.2.
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After 45 µl of 5 | After 205 µl more
of 5M NaOH
Original
After 205 µl more
After 45 µl more
solution
M NaOH
of 5M NaOH
of 5 M NaOH
Actual pH
4.30
6.05
6.90
11.01
11.29
Predicted pH
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