Chemistry Lab Report 3
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Chemistry Lab Report
Name:
Lydia Whyte
Date:
November 1
Experiment #:
3
Title:
Quantitative and Qualitative Analysis
Purpose:
To measure the quantity of a substance, present in a chemical compound via
quantitative analysis, along with determining the identity of chemical substances
through qualitative analysis.
Procedure:
Quantitative analysis 1:
1.
Place test tube inside the inside empty beaker and place on balance.
2.
Tare balance.
3.
Add one gram of metal carbonate and record weight.
4.
Tare balance and add sulfuric acid to the test tube.
5.
Observe fizzing reaction.
6.
Mix until the fizzing settles.
7.
Place beaker on the balance and record the new mass (which will be the mass
lost).
Quantitative analysis 2:
1.
Place crucible container on balance.
2.
Add hydrate to the crucible.
3.
Measure the mass of the hydrate on balance.
4.
Place lid on crucible with the unknown hydrate placed on the Bunsen burner.
5.
Heat crucible for 10 minutes, and hydrate will lose water.
6.
Place crucible in desiccator to cool for 10 minuets.
7.
Place crucible on balance and record mass.
Qualitative analysis 1:
1.
Begin with stationary phase by placing a piece of porous filter paper, then the
mobile phase will follow by a liquid moving by capillary action up the surface of
the paper.
2.
Prepare mixture of amino acids; Aspartic acid, Histidine, Leucine, Phenylalanine,
Proline, and Aspartame
3.
Place cylindrical pours paper in beaker where it does not touch the sides.
4.
Cover the top with foil until the liquid climbs from the mobile phase to the
stationary phase (about an hour).
5.
After an hour, flatten the paper and mark the solvent front grown.
6.
Once it has had the chance to dry spray the paper with Ninhydrin, which will
visualize the spots for us.
7.
After colors have developed mark them and measure the distance each
compound has traveled from the starting line.
8.
Calculate the Rf values from each compound.
Qualitative analysis 2:
Chemistry Lab Report
1.
Apply sample to carbon adhesive tape.
2.
Place sample on the platform in the sputter coat chamber.
3.
Mount the two carbon rods into the sputter coat chamber.
4.
Create vacuum in sputter coat chamber by closing the top. Press the open button
then push down the current button to check vacuum.
5.
Remove the samples from sputter coater and insert samples into SEM/EDX.
6.
Create vacuum in SEM/EDX by operating on a computer under vacuum and
cluck pump. From here we will observe the column pressure being added.
7.
Adjust the gross and fine positioning of sample 1 and magnify view of sample 2.
Data/Results/Calculations:
Quantitative analysis 1:
% CO2 in the sample= (Weight loss / Sample Weight) x 100
% CO2 = (0.7033 / 1.1809) x 100=
59.56%
Compound
Molecular Weight
% CO2 Calculation
CaCO3
100.09
44.01/100.09= 43.97%
K2CO3
138.21
44.01/138.21= 31.84%
Na2CO3
105.99
44.01/105.99= 41.52%
Li2CO3
73.892
44.01/73.892=
59.56%
The experimental loss of CO2 matches Li2CO3 exactly so we know that the unknown
carbonate must be Li2CO3.
Quantitative analysis 2:
% H2O in the sample = (Weight loss / Sample weight) x 100
%H2O= 0.3748 / 2.4406 x 100=
15.36%
Compound
Molecular Weight
% H2O Calculation
AlCl3x6H2O
241.43
108.096/241.43=44.77%
MgCl2x6H2O
203.301
108.096/203.301=53.17%
Na2CO3x10H2O
286.15
180.16/286.15=62.96%
BaCl2x2H2O
244.462
36.032/244.462=
14.74%
The experimental H2O loss best matches that of BaCl2x2H2O, so the unknown hydrate
must be BaCl2x2H2O.
Qualitative analysis 1:
Calculation of amino acid Rf values= Rf= distance amino acid moved / distance mobile
phase moved
Rf (Asp)= 18mm/74mm= 0.243
Rf(His)=22mm/74mm=0.297
Rf(Leu)=55mm/74mm=0.743
Rf(Phe)=51mm/74mm=0.689
Rf(Pro)=33mm/74mm=0.446
Rf component 1: 21mm/74mm= 0.283
Best matches with Rf value and color of Asp (0.243)
Rf component 2: 50mm/74mm= 0.676
Bests matches with Rf value and color of Phe (0.689)
Aspartame= Aspartic acid and Phenylalanine
Qualitative analysis 2:
Unknown 1- The SEM/EDX spectrum demonstrates the presence of the following
elements; C, N, and O.
Chemistry Lab Report
The unknown must be C7H3NO, since it is the only compound containing C, N, and O.
Unknown 2- The SEM/EDX spectrum demonstrates the presence of the following
elements: C, O, and Br.
The unknown must be C2H5OBr since it is the only compound containing C, O, and Br.
Conclusions:
Quantitative analysis 1: Carbonate analysis
By comparing the % of CO2 with other carbonate compounds we were able to
understand that CO2 was lost in the experiment. Since in this specific experiment
59.56% of CO2 was lost we could compare that to known values and with an exact
match we were able to determine that the unknown carbonate was LiCO3.
Quantitative analysis 2: Hydrate analysis
By comparing the % of H2O lost in other compounds we were able to conclude that the
value obtained, 15.36%, was a best match of BaCl2x2H20.
Qualitative analysis 1: Paper chromatography
We were able to conclude that the two unknown amino acids in Aspartame were
Phenylamine and Aspartic acid. We were able to determine this from the two Rf values
calculated in the Data/Results/Calculations section.
Qualitative analysis 2: Scanned electron microscopy
The SEM/EDX spectrum was able to detect C, N, and O in the first unknown sample.
When presented with the possible molecules it could be we were able to infer that it was
C7H7NO because it contained all the elements that were found by the SEM/EDX.
In the second unknown sample the SEM/EDX found C, O, and Br. From this we were
able to infer that it was C7H5OBr due to it being the only compound containing all the
components found in the SEM/EDX.
Notes:
-
The bubbling from the mixture containing sulfuric acid and carbonate was due to
Carbon Dioxide being given off in the chemical reaction.
-
The hydrate lost water because it was holding several moles of water per
compound, and the heat resulted in the water being boiled off.
-
The desiccator has material on the bottom that will prevent water from being
picked up.
-
The crucible can withstand large amounts of heat and will not crack.
-
Paper chromatography is used for dividing mixtures into individual compounds to
allow us to analyze the individual components.
-
Ninhydrin will react with amino acids to form compounds and color them
differently to help us analyze said amino acids.
-
The SEM scans a sample with focused beams of electrons, that will interact with
the sample and synthesize signals consisting of information related to surface
topography and the compounds found in the sample. All elements from B to U
(besides H) can be detected by the EDX combined with the SEM. Sputter coating
is used to make a specimen conductive while preventing the electron beam to
charge the specimen by covering it with a thin layer of conductive material. If the
EDX is used with a sample that has a low atomic number, then carbon coatings
can be used.
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Routine Chemical Tests for Urine - Word
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COLLEGE OF HEALTH SCIENCES
RESULTS AND OBSERVA TIONS:
PARTI: BENEDICTS TEST FOR REDUCING SUGARS
田
TUBE
PATIENT
COLOR
RESULT and
NO.
Before: Blue-green
OBSERVATION
WHITE PRECIPITATE:
1
Normal Patient 1
After: Greenish brown
2
Normal Patient 2
Before: Blue
LIGHT GREEN
After: Green
PRECIPITA TE:
3
WHILTE AND YELLOW
Pathologic Patient 1
Before: Blue
PRECIPITATE:
After: Green
BRICK RED
Before: Blue-green
After: Brick Red
Pathologic Patient 2
PRECIPITATE:
Generalization:
Page 5 of 8
947 words
English (Philippines)
K…
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Data Table
Final buret reading
Initial buret reading
Volume used
Sample 1
Vinegar Base Vinegar
Sample 2
Sample 3
(if needed)
Base
Vinegar
Base
JOML
Som
0,05
110mL/802 10ML 884
JUML 0.00 10μL OML
Tome 0.00
10mL 80.2 mL 10ML 88mL come some
*If a pipet is used to measure the volume of vinegar, record only in the space for volume used.
Molarity of base (NaOH) 1685 M Vinegar number
N/A
CALCULATIONS: In the spaces below, show calculation setups for Sample 1 only. Show
answers for both samples in the boxes.
1. Moles of base (NaOH)
2. Moles of acid (HC2H3O2) used to neutralize
(react with) the above number of moles of
base
3. Molarity of acetic acid in the vinegar
Sample 1
Sample 2
Sample 3
(if needed)
4. Average molarity of acetic acid in the vinegar
5. Grams of acetic acid per liter (from average molarity)
6. Mass percent acetic acid in vinegar sample
(density of vinegar = 1.005 g/mL)
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I. True or False. Write true if the statement is correct and false if it is incorrect.
Use separate paper for your answers.
1. In sterilization process, alcohol solution should be 70%-90%.
2. It is very important to observe safety rules in sanitizing tools and equipment.
3. First thing to do in the preparation of metal implements is to soak the tools in
cold soapy water.
4. Remove immediately tools and implements from disinfectant solution.
5. In storing tools and implements keep unclean and dull.
6. All hair, used cotton or other waste materials must be removed from the floor
right away, and deposit it in a closed container.
7. Each beautician must wear a washable uniform while serving clients.
8. Only VIP's client must be provided with a freshly laundered towel.
9. Finger bowls must be sanitized before another client use them.
10. All waste materials should be disposed of in an enclosed waste bin fitted with
polythene bin liner, durable enough to resist tearing.
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STANDARD SAMPLE PREPARATIONS FOR ABSORBANCE & CONCENTRATION DATA
Concentration of stock nickel sulfate hexahydrate solution = .400 Molarity
Sample
Volume
Absorbance
Concentration (In Molarity)
a
5 mL
.179
10 mL
.329
15 mL
.588
20 mL
.760
25 mL
.939
Reference Blank = 0
Please show how to find Molarity, please show
steps. Thank you and stay safe.
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Question 2
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2. What measurements might you record regarding water samples to assist with the quantification
process? How might these measurements have error introduced?
3. Create a table of your observations made during the experiment. For example:
Sample 2
Sample 1
Initial
After shaking
Write a brief summary of any conclusion that were reached.
4.
With your group, write a sentence statement of the objective of the experiment and the
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36
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command
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ASTRO CAT
Unknown Solid Identification
Prelab Questions
Section CHM lol-217
Anniston Stovall
Name
1. Sodium ion (Na") and calcium ion (Ca2) produce nearly the same color in a flame test (yellow and
yellow-orange, respectively). Describe a way to differentiate between the two using a solution of Na,CO,
and write the correct balanced equation(s). (Hint: recall the solubility rules for Na* ion.)
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$
F4
An aqueous solution of 2.87 M hydrochloric acid, HCl, has a density of 1.05 g/mL.
The percent by mass of HCl in the solution is
%.
Submit Answer
%
5
T
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F5
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ame:
Table 2. Determination of Acetylsalicylic Acid in Aspirin.
1) Aspirin Brand (and amount of acetylsalicylic acid on label)
2) Average molar concentration of NaOH Solution B (from Part A)
3)
4)
5)
6)
Mass of aspirin tablet
Mass of pulverized aspirin sample
Initial buret reading
Final buret reading
7) Volume of NaOH Solution B added
8)
Moles of NaOH
9) Moles of acetylsalicylic acid in sample
10) Mass of acetylsalicylic acid in sample
11) Amount of acetylsalicylic acid per tablet
T₁
1.264
12) Mean amount of acetylsalicylic acid per tablet
13) Standard deviation for acetylsalicylic acid per tablet
14) RSD for acetylsalicylic acid per tablet
15) Relative percent error (%)
16) Is the mean amount per tablet acceptable? (Yes or no.)
0.000153 * 180. Kg
Imol
T₂
√3
1.261 1-259
Seat
กา
Basic 8.25 mg
0.0975 M
Trial 1*
Trial 2
*Show all calculations related to Trial 1, standard deviation, RSD, and relative % error.
Mole of NaOH
(0.0975) (14.3) = 0.000139
(0.0975) (15.7) =
8.000 253
23
0.0975)…
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0.212
2.285
1.890
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c. The calcium carbonate precipitate is weighed today instead of the next lab period? Explain.
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Trial 1
Trial 2
Trial 3
Average
Precision
Mass of unknown oxalic acid (g)
1.0048
0.9995
1.0018
---
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Moles of KMnO4
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9.85
10.45
---
---
---
Moles of oxalic acid (H2C204 • 2H2O)
---
---
grams of oxalic acid (H2C204 • 2H2O) (g)
percent oxalic acid (%)
---
---
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DIRECT ANSWER NO NEED EXPLANATION.
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Mixtures and Pure Substances Lab Data Sheet
Discussion Questions: In the space provided for each question, type your response. Be sure to write
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data sheet as docx or pdf file in Canvas.
1.Classify the salt and pepper mixture.
Click or tap here to enter text.
2.Why did you add water to the salt and pepper mixture?
Click or tap here to enter text.
3.Classify the types of mixtures formed from the salt, pepper and water.
Click or tap here to enter text.
4.What is the physical method used to separate the salt water from the pepper?
Click or tap here to enter text.
5.What is the physical method used to separate the salt from water?
Click or tap here to enter text.
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Click or tap here to enter text.
7.What are the types of pure substances present in the salt solution?
Click or tap here to enter text.
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correctly a yellow face will appear with a smile.
After practicing with the game, balance the given chemical equations without use of the simulation.
Drag the appropriate labels to their respective targets. Leave the target empty for a coefficient of 1.
• View Available Hint(s)
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3
NaOH
O Na,C,0,
4
H,C,0,
Fe, 0,
Fe
co2
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How many molecules of CH¿O6 does a Vitamin C
9.
drink with 1.000 × 10³ mg C6H3O6 contain?
STARTING AMOUNT
ADD FACTOR
DELETE
ANSWER
RESET
*( )
G
176.12
1
88.07
mg
3.419 × 1024
3.419 x 1021
0.001
molec
2.925 x 10-22
2.925 x 10-16
2.925 x 1016
g
2.925 x 10-19
5.68 x 1020
1.000 x 103
mo
6.022 x 1023
5.68 x 10-3
3.419 x 10-2
g/m
6.022 x 1022
2.925 x 1022
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-the top of the balance.
-a folded weighing paper or weighing boat.
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4x zoom
24
Help reading a burette
25
25
26
Sample site B
Final reading
(cm³)
Initial reading
(cm³)
Volume added
(cm³)
Average volume
added (cm³)
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- Please answer fast i give you upvote.arrow_forward$ F4 An aqueous solution of 2.87 M hydrochloric acid, HCl, has a density of 1.05 g/mL. The percent by mass of HCl in the solution is %. Submit Answer % 5 T G F5 Retry Entire Group 9 more group attempts remaining 6 Cengage Learning | Cengage Technical Support Y H [Review Topics] [References] Use the References to access important values if needed for this question. MacBook Air F6 & 7 gilinent-take F7 8 00 1 DII FB K ( 9 F9 0 L ) O A F10 P Chapter Previous + 11 X Next ☐ Save and Exit OWLY S delesarrow_forwardame: Table 2. Determination of Acetylsalicylic Acid in Aspirin. 1) Aspirin Brand (and amount of acetylsalicylic acid on label) 2) Average molar concentration of NaOH Solution B (from Part A) 3) 4) 5) 6) Mass of aspirin tablet Mass of pulverized aspirin sample Initial buret reading Final buret reading 7) Volume of NaOH Solution B added 8) Moles of NaOH 9) Moles of acetylsalicylic acid in sample 10) Mass of acetylsalicylic acid in sample 11) Amount of acetylsalicylic acid per tablet T₁ 1.264 12) Mean amount of acetylsalicylic acid per tablet 13) Standard deviation for acetylsalicylic acid per tablet 14) RSD for acetylsalicylic acid per tablet 15) Relative percent error (%) 16) Is the mean amount per tablet acceptable? (Yes or no.) 0.000153 * 180. Kg Imol T₂ √3 1.261 1-259 Seat กา Basic 8.25 mg 0.0975 M Trial 1* Trial 2 *Show all calculations related to Trial 1, standard deviation, RSD, and relative % error. Mole of NaOH (0.0975) (14.3) = 0.000139 (0.0975) (15.7) = 8.000 253 23 0.0975)…arrow_forward
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