Spectrophotometry Lab Answer Sheet

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Kennesaw State University *

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1112

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Chemistry

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Dec 6, 2023

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docx

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3

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Spectrophotometry Lab Question 1 a. Chlorophyll reflects green light (540 - 560 nm) and absorbs other wavelengths. What biologically important molecules other than chlorophyll absorb and reflect certain colors? Nitrogenous base cytosine, adenine, thymine, uracil, and guanine. b. ls the absorbance of light critical to these molecules' functions or just a consequence of their molecular structure? Explain your answer. Each molecule has a unique structure, which makes them absorb and/or reflect certain wavelengths of light. Question 2 Why is it important to clean the sample tubes? For the light to pass absorb and not reflect on any foreign substance on the sample tube, it must be clean. Wavelength (nm) Absorbance 350 0.41 380 0.64 410 0.65 440 0.35 470 0.32 500 0.46 530 0.78 560 1.34 590 2.01 630 2.25 660 0.82 690 0.14 Peak Wavelength: 630 Concentration Standards (mg/mL) Absorbance 1 0.06 10 0.39 20 0.85 30 1.32 40 1.76 50 2.25 Unknown Absorbance Concentration A 1.55 35 mg/mL B 1.87 42 mg/mL C 1.47 32 mg/mL D 1.16 27 mg/mL Paste your standard curve here:
0 10 20 30 40 50 60 0 0.5 1 1.5 2 2.5 f(x) = 0.05 x − 0.03 R² = 1 Absorption: Standard Curve Question 3 a. What wavelength is the peak absorbance of Bromophenol Blue? 630 b. Why is it important to recalibrate with your blank sample often? In order to get an accurate reading. Just in case you used a different substance previously, you do not want it affecting your current experiment. c. Would you expect a curve of the same shape for another molecule such as chlorophyll? Why or why not? It would be a slightly different curve, because every molecule has a unique pattern that allows it to reflect and absorb certain light. Question4 Do the plotted data points of your standard curve lie on a straight line? Yes Chlorophyll Absorption Spectra Wavelength (nm) Absorbance 350 0.56 380 0.81 410 1.17 440 0.77 470 0.38 500 0.18 530 0.16 560 0.16 590 0.19 630 0.24 660 0.76 690 0.18
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