PhysioEx Exercise 1 Activity 1

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Chemistry

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Jan 9, 2024

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PhysioEx Lab Report Exercise 1: Cell Transport Mechanisms and Permeability Activity 1: Simulating Dialysis (Simple Diffusion) Name: Tia Cronin Date: 3 September 2023 Session ID: session-7cb6969c-81b0-4280-b804-6389eab4aef2 Pre-lab Quiz Results You scored 100% by answering 3 out of 3 questions correctly. Experiment Results Predict Questions Stop & Think Questions The driving force for diffusion is You correctly answered: the kinetic energy of the molecules in motion. 1 In diffusion, molecules move You correctly answered: from high concentration to low concentration. 2 Which of the following dialysis membranes has the largest pore size? You correctly answered: 200 MWCO. 3 Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer: No, not at all. 1 Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mol. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer: albumin only. 2 The reason sodium chloride didn't diffuse left to right is that You correctly answered: the membrane pore size was too small. 1
Experiment Data Solute MWCO Left Solute (mM) Right Solute (mM) Average Diffusion (mM/sec) Na Cl 20 8.00 0.00 0.0000 Urea 20 0.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 20 0.00 0.00 0.0000 Urea 20 9.00 0.00 0.0000 Albumin 20 0.00 0.00 0.0000 Glucose 20 0.00 0.00 0.0000 Na Cl 50 9.00 0.00 0.0150 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 50 18.00 0.00 0.0273 Urea 50 0.00 0.00 0.0000 Albumin 50 0.00 0.00 0.0000 Glucose 50 0.00 0.00 0.0000 Na Cl 100 9.00 0.00 0.0150 Urea 100 0.00 0.00 0.0000 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 100 0.00 0.00 0.0000 Urea 100 9.00 0.00 0.0094 Albumin 100 0.00 0.00 0.0000 Glucose 100 0.00 0.00 0.0000 Na Cl 200 0.00 0.00 0.0000 Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mol. There is no reason to run these solutes at the 20 MWCO because You correctly answered: glucose and albumin are both too large to pass. 2 The rate of diffusion for urea You correctly answered: is slower than that for sodium because urea is a larger molecule. 3 + - + - + - + - + - + - + -
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