neur 327 lab report 7

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Northern Virginia Community College *

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Electrical Engineering

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Dec 6, 2023

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NEUR 328 Week 7 Crescent Loom; Solve a circuit Laboratory Part 1: Orient Yourself 1. Read assigned readings and interact with assigned background material on Blackboard 2. Take the in-class quiz over readings and last week’s lab 3. Open Crescent Loom Connectome explorer here: https://crescentloom.com/connectome-explorer 4. Click the tabs at the bottom of the page to orient yourself. 5. Click the button that says “Launch.” 6. There are multiple creatures stored on the “disk” and “online.” 7. Choose Bendy 501 and let us orient ourselves to some of the tools. a. Notice that the cell types in the brain and connections are blank b. Familiarize yourself with the tools available that will help you to figure out which cells are connected and what they do. c. To excite a cell, use the stimulator: lightning bolt . To inhibit: use SHIFT -Lightning bolt d. Ablate: use this to get rid of a cell entirely for a process of elimination approach e. Pharmacological blockers Bicuculine; CNQX; TTX TTX is sodium Bicuculine is block excitation for AMPA glutamate receptors that prevents cells from exciting one another. CNQX is f. Optogenetic stimulation: g. Please note that the symbols vary for the elements in the brain. h. These guiding questions are for discussion with your instructor. Please answer questions in your lab report worksheet to turn in. i. What do the red diamonds represent? Motor neurons ii. How do you determine what they are? Motor neurons are specialized nerve cells (neurons) within the nervous system that are responsible for transmitting signals from the central nervous system (CNS), which includes the brain and spinal cord, to the muscles and glands in the body.

iii. What is the behavior of RAD when stimulated? When RAD is stimulated, it has an excitatory connection with JAY. 1. Does it fire action potentials? 2. What is the behavior of Bendy 501 when RAD is stimulated? JAY is affected and stimulated when RAD is stimulated. iv. What is the behavior of FAE when stimulated? When FAE is stimulated, it has an excitatory connection with COT. 1. Does it fire action potentials? Yes, FAE does fire action potentials. 2. What is the behavior of Bendy501 when FAE is stimulated? When Bendy501 is stimulated with FAE, it moves in the same position. v. What do you conclude about RAD and FAE? RAD has a more stimulating effect on the creature. Part 2: Divide and Conquer You should work in teams to find connections. Your group will be assigned a neuron to test connections. Please answer the following questions in the Worksheet Lab report. 8. Determine the connections and identity of your assigned interneuron. Answer these questions on your worksheet lab notebook : Cell type? Example: Interneuron LOL is a pacemaker neuron Target(s) Experiment and result (evidence) 1 for target 1 When LOL is stimulated, it activates RAD. Experiment and result (evidence) 2 for target 1. BOO excites COT. JAY requires RAD to be stimulated. Experiment and result (evidence) 3 for target 1 LOL directly inhibits RAD, which is an excitatory connection. LOL has an excitatory connection with JAY because when LOL is stimulated, it results in a positive interneuron response. Nature of connection (inhibitory, excitatory) and evidence for your findings. Excitatory connections promote the firing of action potentials, while inhibitory connections suppress or inhibit the firing of action potentials. Repeat for target 2 if present. Input(s) – Target 2 is interneuron COT which is a pacemaker neuron. . Experiment and result (evidence) 1 for input 1 When COT is stimulated, it activates RAD. Experiment and result (evidence) 2 for input 1 When LOL and BOO interact, they have na inhibitory connection. BOO has an excitatory connection with COT. Experiment and result (evidence) 3 for input 1

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