05.05 Mixtures and Solutions Essay

If you put too much of a sample on your chromatography paper, you could possibly have that color bleed into the color next to it, which would mess up your results. If you put too little of a sample on the paper, your color

baseline (see Figure 9.4 on page 288 for help). If the solvent traveled 57 mm

1) Calculate the solute potential of a .1M NaCl solution at 25 degrees Celsius. If the concentration of NaCl inside the plant cell is .15M, which way will the water diffuse if the cell is placed in the .1M NaCl solutions? .1 M NaCl solution water potential = (-2)(.1)(.0831) (298.15) = -4.96. Increase molarity decreases water potential, so the water will flow into the plant cell.

Add a few drops of food dye to the water until you like the color you see.

It may take several (five to 10) minutes of stirring, so you may need to be patient. Why do you think it's important to start out with a solution that has such a high concentration of salt? • Arrange the five cups on a surface, going in a line from left to right. Label the cups 1 to 5. If you are using plastic cups, you can use a permanent marker to label them.

In thin layer chromatography a stationary phase, silica gel with a glass backing, is dotted on a pencil drawn

Caution – Hydrochloric acid is corrosive to eyes, skin and clothes. Wearing a lab apron, rubber gloves, goggles, and a face shield is essential. Rinse any spills on skin or clothing with plenty of cold water. Clean up spills immediately – ask your instructor for help.

Each pile (equal amounts) of salt goes in a jar. Three jars (one of each temperature) gets a spoon. With the spoons, stir the contents of the jars. Then, take the jars without spoons and swirl them around. Time the swirling or stirring from when you start to when the salt is completely (or as close as it can get) gone.

In this investigation, we are trying to find how far each of the dye traveled on the chromatography paper. In the solvent with the blue dye, the solvent traveled 97mm and the blue dye traveled 81mm. In the solvent with the yellow dye, the solvent traveled 97mm and the yellow dye traveled 70mm. The percent error of the solvent front is 5.8%. The percent error of the distance traveled by the blue dye is 16%. The percent error of the distance traveled by the yellow dye is 26%. The percent error of the retardation factor of the blue dye is 11%.

4. I decide to mask all seagrass before applying the second wash of the underwater background. Using a 2” wash brush, I wet the entire paper again carefully, trying not to disturb the initial wash. I apply new gamboge first at the left side and bottom corner, then move into other water areas. I continue dropping in thalo blue and ultramarine blue wet-in-wet. For areas where the main elements are overlapped, I drop in thick and dark indigo to make them stand out more. Before the wash dries, I add more salt and rubbing alcohol to the left side of the

The objective of this “Chromatography Lab” is to determine the number of substances and their Rf values present as dyes in different markers/inks. Chromatography deals with physically separating a mixture into it’s very own components. Chromatography is used for keeping compounds from mixtures. The process that is involved in it, is separating out a mixture of chemicals, that are most likely either in a liquid or gas form. This process allows the chemicals/substances, to past each other, which is either a liquid or solid.

In Trial 2 and 3, the salt dissolved at the bottom of the glass stay a constant color while the portion above mix into one color, such as Trial 2, purple and Trial 3

Repeat Step 3 to Step 7 using the plaster water bottle filled with the salt solution and record

Transfer the 400ml of salt solute water into 2x empty 200ml beaker, leaving remaining 100ml of salt solute in the 500ml Beaker. Repeat this with the distilled water.

The next step was to place the strip of chromatography paper on a paper towel. Then dip a capillary tube into the plant pigment extract (spinach pigment extract) provided by the teacher. The tube will fill on its own. We applied the extract to the pencil line on the paper, blew the strip dry, and repeated it three to four times until the line on the paper is a dark