1.4.5 Biosensor Biosensor and related bioarray techniques are rapidly growing fields. A biosensor

600 WordsApr 23, 20193 Pages
1.4.5 Biosensor Biosensor and related bioarray techniques are rapidly growing fields. A biosensor is an analytical device that converts the concentration of the target substances into an electrical signal via a combination of biological or biologically-derived recognition systems, either integrated within or intimately associated with a suitable physico-chemical transducer. Essentially, biosensors consist of three parts: recognition, a transducer and a signal output. A biological sensing element with a transducer will produce a signal that is proportional to target analyses. When biological molecules interact with target analyses, there is change in one or more physical-chemical parameters that are associated with the interaction, such as…show more content…
More research is needed to develop an electrochemical approach for the detection of lysozyme; such a biosensor will offer an alternative, sensitive and versatile method for protein detection. 1.4.6 Flow injection chemiluminescence system A novel analytical procedure, based on chemiluminescence (CL) detection, was described for the determination of lysozyme at ng/ml levels using controlled-reagent-release technology in a flow injection system. The analytical reagents involved in the CL reaction, including luminol and periodate, were immobilized on the anion-exchange resins in the flow injection system. Through water injection, luminol and periodate were eluted from the anion-exchange column to generate the chemiluminescence, which was inhibited in the presence of lysozyme. By measuring the decrease of CL intensity, the lysozyme concentration in the range of 30-100 ng/ml could be calculated (Figure 3). A typical analytical procedure, which includes sampling and washing, could be performed in 0.5 min at a flow rate of 2.0 ml/min, giving a throughput of 120 ml/h with a relative standard deviation of less than 3.0%. This method was applied successfully to the determination of lysozyme in human tear and saliva samples and recovery was from 92.0% to 105.7% (Song and Hou, 2003). Flow Injection Chemiluminescence (FICL) analysis is becoming increasingly important in various fields because of its high sensitivity, rapidity, simplicity and feasibility. The fast oxidation

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