The dialysis tubing will be clamped at one end in order to fill it and then clamped at the other end to seal the filled bag. If the bag is not soft and floppy, the experiment will not work. Blot a bag with a paper towel to absorb the moisture and weigh it, if this blotting process is not done it could interfere with the weight readings creating inaccurate information. After the bags of the solutions are prepared, they will be placed into five different beakers with different solutions. Beakers 1-4 will be filled with tap water and the fifth beaker is filled with 40% sucrose and water. Fill each beaker with just enough water or solution so that the bag is covered and place the bags in the beakers simultaneously and record each time. Every 10 min the bags are to be taken out, blotted, and weighed again before returning them back into their respective beaker for another 10 min. The process is repeated until you have reached 90 min. The weights should be recorded in grams (g).
The independent variable was the concentration of sucrose in the dialysis tubing we used as a simulated membrane.
When glucose carriers in the membrane were set to 500, the glucose transport rate for 2.00 mM of glucose was .0008 mM/min. Equilibrium was reached at 43 minutes. At 700 glucose carriers the rate was .0010 mM , and equilibrium was reached at 33 minutes. When the glucose carriers was set at 900 the rate was .012 mM/min, and equilibrium was reached at 27 minutes. After changing the glucose concentration to 8.0 mM, the glucose transport rate with 500 carrier proteins was .0023 mM/min, and equilibrium was reached at 58 minutes. With the simulation set at 700 carrier proteins the rate was .0031mM/min, and equilibrium was reached at 43 minutes. When the simulation was done with 900 carrier proteins the glucose transport rate was .0038, and equilibrium was reached at 35 minutes.
The hypothesis states that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant. In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution made during the study, four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis.
Using the graduated cylinder, measure 20mLs of the stock sucrose solution and 180mL of water to create a 3% sucrose solution and place it into the 250mL beaker (beaker #2). Place bags #1‐3 (red, blue, yellow) into beaker 2 and bag #4 (green) into beaker 1. Allow the bags to sit for one hour. After allowing the bags to sit for one hour, remove them from the beakers carefully open the bags, noting that often times the tops may need to be cut as they tend to dry out. Measure the solution volumes of each dialysis bag using the empty 250 ml beaker.
Three peaks are observed in Figure 1 (concentration of glucose vs. elution volume) which was expected due to the results in table 4 that show intervals of elution. The intervals of the elution are represented as peaks on the graph. The intervals are due to the glucose molecules that enter the beads of the column causing the glucose molecules to elute slowly. Two peaks are observed in Figure 2 (concentration of starch vs. elution volume), which was not expected. One peak was expected for the
There were several steps completed to prepare for the experiment. Three dialysis tubes were filled with approximately the same volume of distilled water and then were tied shut. The initial mass (in grams) of the tubes was taken using a triple beam scale. I then filled three 500 mL beakers with 400 mL of water each and dissolved different masses of solute (table sugar) in each beaker in order to make 5%, 10%, and 20% solutions. The beakers were labeled accordingly, and then 20 g, 40 g, and 80 g (respectively) of table sugar was weighed out using a digital scale and placed into the corresponding beakers. The sugar was stirred in using a stirring rod until all of the solute was completely dissolved.
From figure 2, we can observe diffusion taking place.’S’ represent starch, ‘i’ represents iodine and ‘g’ represent glucose. As initially, 1 inch dialysis tube consists of iodine and glucose and 3 inch dialysis tube consists of starch. However, after 1 hour, the iodine molecules got diffused into 3 inch dialysis tube and also the water in the beaker turned slightly cloudy in the final stage.
Table 1. Demonstrating the amount of glucose in each test tube Temperatures Test Tube 1 ±50 test tube 2 ±50 Test tube 3 ±50 25oC 550 500 500 37oC 650 700 650 0.8oC 300 350 350 100oC 0 0 50 Positive glucose Control, 25oC 900 900 850
First, we collected the materials that were needed: 3 beakers, 2 clips, glucose test strips, cooked starch, amylase, and iodine. Then we put 4 pipettes of cooked starch in the small beaker. We then mixed the starch wit h4 pipettes of amylase. The starch will be the leaf; the amylase will be the digestive enzyme. The small beaker represents the head and crop. We then put 4 pipettes of the “macerated” solution from the crop to the soaked dialysis tubing. We filled the large beaker two-thirds full of water and put 4 drops of Lugol’s to the beaker. We then put the dialysis tubing into the large beaker. We recorded data
In the final experiment we filled a dialysis bag with starch solution and tied off both ends of the bag so that it is water tight. We then filled a separate bag with sodium chloride and submerge both dialysis bags in two beakers of distilled water. We allowed the bags to sit in the water for 10 minutes. We then put silver nitrate into the water that held the dialysis bag filled with sodium chloride and recorded any changes in the water. We then added iodine to the water that held the dialysis bag of starch and observed any changes in the water.
In the experimental group, the dialysis tubing with starch and amylase was observed. The initial color of the solution inside the dialysis tubing and the color after 1 hour was recorded. The start color was clear and 1 hour color was black. The color of the water with Lugol’s in the large beaker was also observed and recorded. The start color was orange and the color after 1 hour was slightly darker orange. The glucose test was green for the dialysis tubing and blue-green for the