2 Aminoanthraquinone Synthesis Lab Report

The design of this experiment aimed to synthesize 2-methylphenoxyacetic acid from 2-methylphenol and sodium chloroacetate by means on phenol alkylation. 2-Methylphenoxyacetic acid has been used to control the growth in plants and exemplifies a product of synthesis as it is a complex molecule constructed from two simple molecules. This synthesis proceeds by a nucleophilic substitution reaction, more specifically, via SN2 mechanism (see below). An SN2 mechanism's reactants are a nucleophile and a good leaving group (usually a halide ion). Once the crude product is obtained, it is purified by recrystallization with water and collected.

Science A CH1HP H Unit Chemistry C1 Chemistry Unit Chemistry C1 Monday 10 June 2013 1.30 pm to 2.30 pm Mark 1 2 3 4 5 6 7 TOTAL For this paper you must have:  a ruler  the Chemistry Data Sheet (enclosed).

To confirm the presence of amides, a medium absorption near 3400 cm-1 should be observed which is the case in this graph.

Benzophenone Structure (Less polar): Benzoic Acid Structure (More

This allows scientists to determine if compounds have desirable structures, and if reaction conditions of unwanted products can be altered. However, with complex compounds that were never characterized, this process is insufficient. Instead, IR Spectroscopy is used. It measures the vibrations of bonds, producing a spectrum of the different energies absorbed by the molecule. By comparing the energies with standard correlation values, the identity of an unknown compound can be determined.

Olmsted, John III; Williams, Greg; Burk, Robert C. Chemistry, 1st Canadian ed.; John Wiley and Sons Ltd: Mississauga, Canada, 2010, pp 399 - 406

CHE 133 Experiment 3, General Chemistry II Lab, Spring Quarter 2014-2015, DePaul University. [Online] https://www.d2l.depaul.edu (accessed April 25, 2015)

Materials and Methods Purification of NADH: Ubiquinone oxidoreductase (Complex I) CMI was purified from E. coli overexpression strain ANN0221 which was kindly provided by Dr. Friedrich (University of Freiburg, Germany) using a modified various of previously described methods(11). All chromatographic steps were performed using a GE ÄKTA pure 25 or ÄKTA avant 125. The anion exchange step was omitted, and affinity chromatography was completed using a Tricorn 10 mm x 100 mm (GE Healthcare Life sciences, Inc.) column packed with High-Performance Ni2+ resin (GE Healthcare Life sciences, Inc.) The sample was adjusted to final imidazole concentration of 50 mM and 200 mM NaCl, followed by sample loading at 153 cm h-1; the column was washed with 150 mM imidazole and CMI was eluted with 375 mM imidazole step gradient at 76.5 cm h-1 in up-flow operation.

Complexation of porphyrin with the metal will cause the shifts of Q bands in the UV-Vis spectra in the range of 500-700 nm as shown in Figure 2.7. This can be observed by the disappearance of two Q bands out of four as the porphyrin undergoes the change from D4h symmetry to D2h symmetry (Rita Giovannetti, 2012). The Q-bands were denoted as α and β. The coordination of porphyrin to Ni(II), Sn(IV) or Pd(II) which can form stable square planar complexes generally consists of a higher α peak (Figure 2.7 (A)), while the coordination of porphyrin to Cd(II) which forms a complex that can easily be displaced by protons will show a higher β peak (Figure 2.7(B)).

In addition, the wavelength of maximum absorption is influenced by the presence of a unique chromophores (light absorbing group) that are also used for bonding to the titanium surface. By comparing the wavelengths of maximum absorption with in the absorption spectrum with those that are known, the structure of the molecules will be fairly determined. A UV-vis-NIR spectrophotometer will be used to confirm the presence of anthocyanins in the dye extracts if they are found to absorb light within the range 500 nm and 550 nm and to determine the light absorbing group as well as to determine molar extinction coefficient. The structure of the compounds in the dye will be confirmed using density function theory

It suggests that the formation of acceptor-donor systems between doped fullerene interacting with amino acids. Excited binding energy can be used to calculate the transport properties of the doped fullerene with amino acids. The larger interaction between the carbon cages and a biological compound leading to novel medical applications [7, 8]. Density Functional Theory is the least expensive and more accurate ab-initio method, DFT studies has been investigated for the title compounds. The theoretical evidence can enhance surface properties to improve biochemical

(equation 2) All the data and analysis will be shown in Result and Discussion part below. Ca^(2+) (aq)+EDTA^(4-) (aq)→〖[CaEDTA]〗^(2-) (aq) (1) 〖[Ca(In)]〗^- (red)+〖H_2 EDTA〗^(2-)→〖[CaEDTA]〗^(2-)+InH^(2-) (blue)+H^+ (2) Run Atomic Absorption Spectroscopy (AAS) on sample (1:20 dilution) with HNO3 (2 mol/L).

Cloudy ammonia is combined with water to form NH3(aq) + H2O(l) NH4 + (aq) + OH‾(aq), The electronic arrangement in nitrogen obeys the octet rule. The four pairs of electrons (three bonding pairs and one non-bonding lone pair) repel each other, giving the molecule its non-planar geometry. In aqueous solution, ammonia acts as a base, acquiring hydrogen ions from H2O to yield ammonium and hydroxide ions. The ammonia molecule has a trigonal pyramidal shape, with nitrogen connected to the three hydrogen

(CH3 and CH2), respectively, 3367 cm-1 corresponding to vN-H;1063, 669.02 cm-1 for the asymmetric and symmetric stretching N+–C as seen in fig.1. the 1HNMR spectroscopy

As a conclusion, we were not able to recover a final product due to misreading the experimental procedure. To avoid this problem in the future, I am planning on double checking my work and being completely positive before disposing of any solution or substance. However, if we would have successfully synthesized resacetophenone, we would have analyzed the identity of the compound by obtaining a melting point range. Then we would have calculated percent yield to see how much product was recovered in comparison to the beginning amount of chemicals present.