3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution

2276 Words Jul 12th, 2013 10 Pages
Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution |
Objective:
1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions.
Apparatus & Equipment’s:
Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath,~37°C Water bath,~95°C Stop watch Test tube holder
Materials:
Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3M Hydrochloric acid 3M Sodium hydroxide
Procedures:
1. Prepared two boiling tubes
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| The blue solution was changed to reddish brown and brick-red precipitate was formed. | 4 | 2ml solution B2ml saliva | 95 | The blue solution was changed to yellowish-brown and brick-red precipitate was formed. | The blue solution was changed to dark brown and brick-red precipitate was formed. | | | | | |
Discussion:
All living organisms need nutrients to supply energy for their daily activities. A nutrient is a chemical that an organism needs to live and grow or a substance used in an organism’s metabolism which must be taken in from its environment. They are to build and repair tissues, regulate body process and are converted to and used as energy. Therefore, most nutrients are used to produce ATP such as carbohydrates, fat and the others. So, we need to break down foods into its most simple forms in order to absorb the nutrients through our digestive system. In this experiment, we will test the action of saliva and hydrochloric acid in two carbohydrate solution with different of temperature. In this experiment, carbohydrate solution A acts a reducing sugar. Benedict’s test and iodine test were carried out at the same time. In the Benedict’s test, Copper(II) oxide is one of the components of Benedict’s reagent. When it is added in carbohydrate solution A(reducing sugar), the blue colour of copper(II) oxide is reduced to insoluble red-brown copper(I) oxide ions. That was caused by redox
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