5-Ima Lab

2. The subject who was diagnosed with secondary hypothyroidism was given levothyroxine (synthetic Thyroxine). After 6 weeks of

Why are spindle fibers important for mitosis? (5 points) Chromosomes use spindle fibers as pathways to daughter cells during mitosis.

Lab 2.1 consisted of an assessment that evaluated my overall health and whether I was healthy enough to begin a fitness program. Based off of the information in this lab it was determined that I have no physical or health related conditions that prevent me from beginning a fitness program. I determined from this lab that since I am already active I would benefit the most over the course of this class from increasing the duration and intensity of my activities (Fahey, Insel & Roth, 2013, pg.48) and to maintain an overall healthy diet. The area I saw that needed a change was the amount of alcohol I consumed over the weekend. I have since cut that down to 1-2 drinks per week. I still workout five days a week and maintain a healthy diet. The changes

Business impact analysis (BIA) is a systematic process to determine and evaluate the potential effects of an interruption to critical business operations as a result of a disaster, accident or emergency. A BIA is an essential component of an organization's business continuance plan; it includes an exploratory component to reveal any vulnerabilities and a planning component to develop strategies for minimizing risk.

All of our water samples were collected from different areas. Sample 1 was collected from a home in Fostoria Ohio that has well water. Sample 2 was taken from Honors Academic Village here on campus. Sample 3 was taken from a home in Haskins Ohio that receives their water from the city of Bowling Green Ohio. Sample 4 was also taken from campus, but from a drinking fountain in Bowman Oddy Laboratories.

You are a newly hired desktop technician for Contoso, Ltd., working on a long- term test deployment of new Windows 7 workstations. You have been given the task of testing the various remote administration technologies included with Windows 7.

The study was conducted by comparing the frequency patterns and the development of breast cancer cells in both groups of mice, the first group was given triclosan and the second

Annexing V FITIC/PI was used to detect for the involvement of apoptosis based upon externalization of phospholipid phosphatidylserine (PS) and permeabilization of nuclear membrane for staining with PI. PS is a biomarker of early apoptotic stage, Annexin V has specific and high affinity for the anionic PS, when PS is exposed on cell surface Annexing V binds to it. PS indicates intermediate stages of apoptosis. Caspases-3 activation, nuclear fragmentation and mitochondrial membrane flux preceded PS translocation [46]. From Fig.5 sonicated pectin induced dose apoptosis and reduced proportion of viable cells. The 400W sonicated pectin was more potent apoptosis inducer than 200W sonicated pectin. control cells had 89.29% viable cells 3.14% early apoptotic cells and 6.50% late apoptosis, 400W sonicated pectin at 0.1 and 0.5mg/ml induced significant reduction in viable cells to 70.90%, early apoptotic cells were 4.84% and late apoptosis 14.94% in the cells as shown in Fig.5b, on the other hand, 0.5mg/ml led to 50.79% viable cells, 22.65 %early apoptotic cells and 19.56% late apoptotic cells as shown in Fig.

The photodynamic qualities of the PUVA light therapy utilize nonhazardous and light-sensitive compounds, which are carefully exposed to the UVA light. The mixture of both the nonhazardous compound and the light form a toxic mixture that is turn breaks down the malignant cells of the skin. When psoralens are combined with the UVA light, they are highly effective at clearing depigmented skin patches formed from vitiligo. This is done by increasing the reactivity of the melanocytes, which reside directly under the skin layers of the white-pigmented patches to the UVA light20. Melanocyte cells have a certain property built into their cells mechanism, which can detect UV light occurrence and trigger its functional properties to bring back natural skin color and pigment on a person. The natural skin tone in turn can efficiently protect against the destructive properties of UV light. The initial dosage of the psoralen is determined by the spread of patches across a person’s body and overall severity of the case. Progressively with more treatments, the dosage of psoralen also increases, adding more and more sensitivity properties to the melanocyte cells. The ultraviolet light therapy, PUVA is currently the most effective at treating vitiligo21. Physicians often perform PUVA light therapy alongside the usage of topical treatment in order to better enhance the effects of the light

In this experiment, analysis of cell viability will be achieved through an MTT assay. The salt 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) is often used in cancer drug screening  (Vistica et al. 1991) as it is

Experimental study made by Iglhaut et al. After 14 weeks, revealed histologically the presence of PDDL trace particles in most augmented sites, but not associated with any inflammatory cell infiltrates in the adjacent tissues.

6-Thioguanine (6-TG) is an anti-cancer drug popularly used for the treatment of leukemia and it is a known photosensitizer that induces the formation of reactive oxygen species (ROS) in the presence of UV light.1-7 Glutathione (GSH) is an antioxidant found in the body that helps prevent damage to cells caused by ROS.2 ROS have the ability to create lesions in DNA after prolong use of 6-TG and may lead to skin cancer when patients are exposed to natural sunlight. 3-5,7 Specifically when patients are exposed to UVA light (315-400 nm) they are at a greater risk.3 Normal DNA absorbs UVB (280-315 nm) and UVC (100-280 nm), both of which are blocked by the ozone layer and therefore pose a miniscule threat to human DNA.4, 5 6-TG is analogous to the purine Guanine. The two differ slightly at the functional group present on the sixth carbon.

Given that taxol and vinblastine disrupt the progression of mitosis and that chromosome condensation is a critical process in mitosis, it is likely that these drugs have an effect on this critical process14. As such, it is intuitive to investigate the effects of drugs on the manifestation of this process, which is the integrity of the chromosomes. Moreover, as compromising the integrity of chromosome reduces cell viability, it is very probable that the chromosomal integrity was altered during the prolonged mitotic delay, which leads to the eventual cell

Acne is a widespread disease affecting most of the population at some point in their lives. Although there are numerous drug alternatives for the treatment of acne; antibiotic resistance and topical or systemic toxicities are the frequent obstacles limiting therapeutical options. This clinical trial aims to determine the efficacy and tolerability of a new topical antiacne formulation containing a herbal extract composed of two Brassica oleracea variants which are reported as having strong antiinflammatory, antimicrobial, antioxidant properties and were not studied clinically before. Herbal extract was prepared by boiling the plant parts and then cooling, filtering and concentrating the preparation. The extract was incorporated into an antiacne

UV radiation can produce apoptosis in cells however it was shown through c-Jun wildtype and c-Jun null mutants in varying quantities of UV that mouse fibroblast cells lacking c-Jun had greater levels of cell death. This occurrence could be seen with a number of the different UV quantities and over many time points and was confirmed to be apoptosis through DNA end-labelling assays. The paper suggested that the c-Jun could represent a checkpoint stopping growth and allowing DNA repair or it could act to help initiate production of genes that inhibit cell death.