A Body And Protective Facial Equipment

The purpose of blood culture draw is to test for the existence of bacteria and other microorganisms that could possibly be in a patient’s blood. Even the smallest contamination will have major adverse effects on the final results of this kind of test. The second in the order of draw is the one use for a coagulation assay. The drawing tube that is use for a coagulation assay has a light blue top. When it comes to coagulation assay, there is an exemption to the order of draw. If it is believe that tissue fluids or thromboplastins have infected that blood, then a non-additive tube may be used in replace of the tube for the coagulation assay. The non-additive tube usually has a red top on it. The third tube in the order of draw is the non-additive, which I said earlier is marked with a red top. This tube is for blood draw taken for testing without adding any kind of reaction additive. The fourth and final draw in the order of draw is an additive tube. There are many different additives that can be used for testing purposes. The final measure of the order of draw has an order in itself that must be followed. The first tube used for additive is the one that contains SST. This tube usually has a red-gray or gold top on it. This tube has additives that have gel separators and clot activator. This tube is used for the testing of the pancreas function in the patient.

6. The disks in the 0.00% solution were transferred to an agar plate held next to the blue flame using the sterilized tweezers. Excess disinfectant was removed from the disks by wiping on the side of the well of the spotting tile. When the 5 disks were positioned (refer to Figure 1 below) the lid was replaced and sticky taped down. A label was added indicating the concentration of disinfectant.

1.2 ml of the bacterial cultures are taken to pellet the cells and remove supernatant while

Dress properly and appropriately in the lab. Take all precautions to lessen the measure of any substance that you are presented

Prior to each spirometry session a 3Litre test syringe is used to calibrate the micro lab spirometer

General Biology Bryarra Tinoco BIO102 - Spring Semester – 2015 February 24, 2015 Introduction For this lab the experiment was to test how effective the antibiotic properties were in four substances. The hypothesis that I conducted for this lab was that if the disks containing the substances had a zone of inhibition then the antibiotic effect could be measured and compared.

Upon beginning the unknown lab, I was given the opportunity to choose an unknown broth tube. This tube contained our unknown microorganism and it was my job, based upon the testing methods learned throughout the semester to distinguish which microbe I had chosen. The goal at this point was to create a reserve plate and a working. This was completed by inoculating one TSA plate thoroughly in order to secure a lot of the growth. This plate became my reserve plate. The working plate was created by using the Streak Method that was introduced at the beginning of the semester. With this method, I would soon be able to visualize and record my microbial growth and colony characteristics. These plates were incubated for a week as that is the time span between classes.

We selected two locations for sample collection: for Site 1 a phone was selected, and Site 2 a desk. One agar plate was labeled “Control” with our group identification number. A sterilized swab was dipped into a test tube with sterile water to wet the cotton on the swab. The culture from the swab was transferred to the surface of an uncultured nutrient agar plate using a zigzag motion. This is to determine if there are any bacteria in

First a thin smear of bacteria, placed on a clean slide, covered with crystal violet stain for 1 minute. Next, completion of a wash and then an iodine solution is added to the smear to enhance staining for 1 minute. Being rinsed again in water and then 95% ethanol for one minute. Washing the violet stain off will differentiate between the two types of bacteria. If the bacteria retains the violet color, it means they are gram positive, while the others without strain are gram negative. The cell wall of the gram positive bacteria has a thick outer layer made up of peptidoglycan and the cell plasma membrane causing the stain to stick. The gram negative bacteria has an outer layer made up of lipopolysaccharides and proteins and then a thin layer of peptidoglycan and the plasma membrane. The lipids from the outer membrane dissolve with the ethanol wash, causing them to lose the violet stain. After the ethanol wash, the smear is washed in water and stained with safranin for one minute allowing for the gram negative bacteria visualization. Before the

The specific infection gets tested with a blood smear in VA screening plates that contain (BHI) agar, so the Lavender Top Tube is used.

I examined all three slides under the microscope. After observing the slides I flushed the specimens down the drain and disinfected the area with bleach solution. After 42-72 hours I assessed the growth patterns of the tube and noted that the tube that contained the swab became yellow stained and the tube that contained the agar and tablet dissolved a little and some of the content drifted to the surface of the tube and created a film.

From one of my modified streak plates I was able to successfully isolate my Gram positive bacteria. By my 6th plate, there was a distinction between my Gram positive and Gram negative in morphology. To make sure it was absolutely pure I continued to streak it two more times. Once I had a pure plate, I Gram stained it twice to confirm that it was a pure Gram positive coccus. Once it was pure I proceeded to do the catalase test, the bacteria produced bubbles, indicating a positive result. From there I was able to narrow down my unknown to a possible of 4 bacteria. By analyzing my matrix I was able to determine that by doing an Oxidase test, it would eliminate Micrococcus luteus. However, the oxidase test turned positive, allowing me to confirm that my unknown Gram positive bacteria was Micrococcus

After allowing the two unknowns to incubate on the streak plate (Picture B), the next step was to gram stain, and hopefully separate them. After following the procedures for gram staining, and observing my slide underneath the microscope, it was evident that I had two microbes on my slide. One of the microbes was gram positive with a coccus shape, while the other was gram negative with a small rode looking shape to it (Picture A). The next step was to be able to separate them from each other using selective media. For the gram positive unknown, I used a MSA plate, which prevented gram negative growth. For the gram negative unknown, I used an EMB plate, which would inhibit any gram positive growth. The next class period on 4/18/17, my gram negative

The microbe that I received was on plate B section 5. I began the project by creating pure cultures of my microbe on a sterile nutrient agar plate, a nutrient agar slant and an agar broth by using Alderson’s procedure for aseptic techniques (Alderson, 2015, p. 53). After inoculating the media with my microbe, I incubated them at 37°C for 48 hours to allow for growth. After 48 hours, utilizing Alderson’s technique for making smears from solid cultures I created 3 smears (Alderson, 2015, pp. 93-94). Using these smears, I created a gram stain (Alderson, 2015, pp. 100-101), a spore stain (Alderson, 2015, pp. 114-115) and an acid-fast stain (Alderson, 2015, pp. 120-121). Despite using Alderson’s techniques for creating the smears and stains, my techniques differed slightly. When creating a smear, Alderson’s technique says to spread the inoculated drop of water to allow the slide to dry however, for the purpose of speeding up the drying time I placed my slides in the ____. In addition, when heat fixing my slides I used a clothespin to hold the slides over the flame when heat fixing. When creating my stains, contrary to Alderson’s techniques, I did not blot any of my slides

Specimens are taken aseptically, utilizing specific control guidelines, to prevent environmental contaminants or normal flora that is unrelated to the infection, from influencing the results. The manner in which a specimen is taken can influence the accuracy of the results and therefore skew the appropriate treatment. There are specific guidelines for how each type specimen is collected, handled, stored and cultured. General aseptic procedures should always be utilized, such as using sterile collection containers to prevent contamination from the environment. It is also vital when collecting a specimen, to only gather a sample from the infected area, as the surrounding areas may contain other bacteria or normal biota. Taking a specimen aseptically helps to ensure there in no cross contamination and helps to isolate the pathogen causing the infection. Using a catheter from the bladder to get a urine sample, is an example of taking an aseptic sample.