A Brief Note On Aetiology Of The Disease

The first result of importance was the result of the Gram stain. The observations of the unknown bacteria from the slant culture after Gram staining showed that the unknown bacteria were Gram negative bacilli (Image 1). After determining the unknown bacteria was Gram negative, an oxidase test was conducted on a sample from the slant culture. The cotton swap with the sample of bacteria did not change color when the oxidase reagent was applied, thus providing a negative result. With a negative oxidase test, further tests were conducted to determine various characteristics of the unknown bacteria. A MR-VP broth was inoculated with a sample from a slant culture of unknown bacteria. After incubation, the methyl red reagent was added to the broth, and the broth turned red, providing a positive result (Image 2). An EMB agar streak plate was inoculated with a sample from a slant culture of the unknown bacteria, and after incubation, growth was found on the plate, providing a positive result (Image 3). A Citrate agar slant was inoculated, and after incubation, growth was found on the media, providing a positive result (Image 4). A Urea agar slant was inoculated, and after incubation, the agar had changed from a peach color to a bright pink color, providing a positive result (Image 5). Using the flowchart (Figure 1) developed from the Table of Expected Results, the lab partners started at the oxidase test. Given the negative result of the oxidase test, the flowchart is

A sample of the patients bacteria causing the urinary tract infection can only be used if a urine sample is bacteriologically viable (1 Radar). Infected urine and sterile urine are common samples used for the diagnosis of patients (1 Radar). It is important to gain further knowledge of multiple organisms’ resistance to antibiotics that cause urinary tract infections (1Radar). Escherichia coli has distinct characteristics and structures that help identify their unique properties (2 Johnson). Escherichia coli is the most common and most prevalent bacteria present in the fecal flora of the human colon (2 Johnson). Because of Escherichia coli unique ability to overcome the obstacles of a new environment, it makes easy for bacteria to spread into the urinary tract and produce a discomforting infection (2 Johnson). All research conducted lead to the belief that Escherichia coli was the Enterobacteriaceae pathogen causing the UTI in the patient. In order to confirm this prediction a set of differential tests and biochemical test must be conducted.

This informed me as to what tests to perform next. The next test was for lactose fermentation. I accomplished this by streaking my bacteria on a MAC plate. Pink colonies on a MAC plate would indicate that the bacteria growing on that plate could ferment lactose, this in addition to the fact that it would inhibit the growth of gram positive organisms made the MAC plate differential and selective, as well as perfect for assisting me in identifying my organism. The results of this test were negative on account of the fact that no pink bacterial colonies were produced, indicating that they were unable to ferment lactose.

Because of the positive nitrite and small amount of leukocytes I would say that could indicate a urinary tract infection.

During the purification section of this lab, the LB/amp/ara agar plate was examined for well-isolated green colonies and the LB/amp plate was observed for white colonies with space between each other. These colonies were circled on the outside of the plates using a marker. Next, two 15 milliliter culture tubes containing 2 milliliters of nutrient growth media were obtained and labeled “+” and “-“. Using a new inoculation tube, the circled colonies from each plate were scooped out and immersed in their respective culture tubes. Once the bacteria was mixed into the solution, the tubes were sealed and placed horizontally into the 32⁰ incubator for 24 hours.

| Term for any infection in the urinary tract, including cystitis and pyelonephritis. It can be

The two types of urinary tract infections are infection of the bladder (Cystitis) and infection of the urethra (urethritis). Cystitis is a type of UTI which is caused by Escherichia coli, a type of bacteria that is normally found in the gastrointestinal tract (www.webmd.com). Although sexual intercourse can cause this type of urinary tract infection, it doesn’t necessarily mean that you have to be sexually active to develop it. Every female is at risk for this type of UTI due to the shortness of distance from the urethral opening to the bladder and the urethra to the anus.

A positive bacterial growth on nutrient agar plate used as control as well as bacteria colony morphology. The EMB was used to select for coliform gram negative bacteria and determine if the bacteria is able to ferment lactose. The Purple color colonies indicated a positive result for strong fermentation of sugars by fecal coliforms. The last plate is the PEA plate, white showed inhibited growth of the bacteria indicating that the unknown bacteria is gram negative.

Whenever there is an unknown disease caused by microorganisms, tests are usually made in order to identify the organism causing the disease. There are several tests that need to be made and they include tests such as performing a gram stain, streaking a plate to isolate colonies, inoculating a broth culture, inoculating API strip, and performing oxidase and catalase tests. Having knowledge on how to identify these tests are of high importance in the medical field so it would be to the advantage of those individuals who know how to examine microorganisms and be able to identify it by correctly performing tests on organisms.

A total of 13 tests were performed on the unknown bacteria culture #1. Two tests were inconclusive and could not be used. The first test was simply the phenylethyl alcohol agar (PAA) to check for contamination because of its selectivity towards gram-positive bacteria. The alcohol in the agar penetrates the thin peptidoglycan wall of gram-negative bacteria, which results in a slowed and stopped growth. The mannitol salts agar (MSA) is a differential test due to the mannitol sugar. It also has high amounts of salt (7.5% NaCl). If the organism can ferment the sugar, and produce acid as a result, the phenol red pH indicator dye will turn yellow due to the acidic environment. If no change occurred, then it is a negative result meaning that the bacteria

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a rare syndrome in the U.S. that primarily infects females but may also be observed in males on occasion. The syndrome is multifunctional and is symptomized by pelvic pain, urinary frequency, and urinary urgency. Also known as painful bladder syndrome, this condition can cause bladder ulcers and/or bleeding resulting in pain during sexual intercourse and hematuria. In December 2014, researchers from the Department of urology of the Beaumont Health System in Royal Oak, MI, the Department of Urology of the Oakland University William Beaumont School of Medicine in Rochester Hills, MI, and Lipella Pharmaceutical Inc. in Pittsburgh, PA published a manuscript sharing the results of an open-label

UTI is an infection of the urethra, bladder, ureters and kidneys that occurs when bacteria gain access to the urinary system (Dailly, 2011). This makes a patient's urine to contain bacteria during a laboratory test, and this bacteria is more prone to being active when

Coli bacteria. In adjustment for an infection to occur, the E. Coli bacilli has to attach itself to the beef lining the float or the urethra (the tube that carries the urine out of the body). The ache of E. Coli that has been the a lot of acknowledged at causing a urinary amplitude infection has developed a circuitous set of adhering molecules that act like tiny assimilation cups to attach themselves to the tissue of the urinary tract. Once those adhering molecules become absorbed to the tissue of the urinary tract, they anon alpha to reproduce. This causes affliction and the archetypal affection of an infection like burning, aching urination, and a faculty of coercion to urinate. If the infection spreads to the kidneys, it can could could cause added problems like lower aback pain, fever, and

This report represents my individual effort. I did not receive or offer aid to anyone when performing this assignment, nor did I plagiarize any material.

The difference between good health and disease is that health is the level of functional or metabolic efficiency of a living being. In humans, it is the general condition of a person's mind, body and spirit, usually meaning to be free from illness, injury or pain (as in “good health” or “healthy”) whiles a disease is an abnormal condition affecting the body of an organism. It is often construed to be a medical condition associated with specific symptoms and signs that may be caused by external factors such as infectious disease, or by internal