A Brief Note On Biotechnology And Biotechnological Equipment Essay

1. Describe the function of the following pieces of safety equipment and how each might be used: (10 points)

3. You test another new unknown bacterial sample, and find the G+C content is identical to one of the samples you have already identified, but the rRNA gene sequence contains one base that is different. What can you conclude: C.

In order to test whether the curry leaf possesses one of the 6 alleles and whether it has a Ruby gene promoter, PCR and gel electrophoresis was used. Primers, short DNA sequences designed specifically for each allele of the Ruby gene, were used to isolate the gene and amplify it for PCR. Multiple PCRs were performed, each using a different primer set. Primers attach to specific regions surrounding the gene and amplify only the region that is flanked. The forward primer is bound to a specific sequence before the Ruby promoter, found to precede the actual gene sequence, and the reverse primer is bound to the DNA after the gene. In order for visible bands for gel electrophoresis to be produced, a forward primer must bind to one strand of DNA and a reverse primer must bind to the strand complementary to the one the forward primer binds to. If both primers bind to the same strand, the PCR product formed will not produce a result after gel electrophoresis. The size of the gel band of the DNA amplified by the primer will determine the identity of the allele. Band sizes will

The levels of the N protein of the virus were determine through ELISA test. Treatments consisted on: a) A. thaliana Wilt Type Columbia – Buffer (Mock); b) A. thaliana Wilt Type Columbia –TSWV; c) A. thaliana mutant dorn1-3 – Buffer; d) A. thaliana mutant dorn1-3 – TSWV; e) A. thaliana line oxDORN1- Buffer; f) A. thaliana line oxDORN1 -TSWV. Inoculation was made 28 to 30 days post-emergence, A. thaliana seedling leaves were manually inoculated with TSWV, either at abaxial and adaxial surface. To test the level of infection, inoculated and non-inoculated young leaves were harvested and frozen 16 days post-inoculation (dpi), and tested by DAS-ELISA using a commercially kit for TSWV (Agdia Inc., Elkhart, IN, USA) following manufacturer’s instructions. The results of ELISA test showed there is not difference between treatments inoculated with

In my project I will explore how biotechnology as a tool of body manipulation and enhancement can redefine the contemporary notion of the human and life in a more ethical and aesthetical way. My argument will address ways in which art that engages with biotechnology as its medium, can give a more tangible because ethically and aesthetically combined understanding of life and the human body. I will focus on selected case studies that work with biotechnology to study ways in which art, can reveal the power of biotechnology to act and produce meaning. This allows me to research how biotechnology can reconstruct and reshape the notion of matter as active and capable of producing meaning.

Leaf tissue was collected from all the 138 ILs along with parents grown in control condition with no salt stress. The genomic DNA was extracted from the ground tissue using CTAB method (Chen and Ronald 1999). The concentration of DNA in each sample was estimated by a ND-1000 spectrophotometer (Thermo Fisher Scientific, Wilmington, USA). The DNA concentration of all samples was adjusted to a final concentration of 25ng/µl for PCR amplification. Genotyping of the ILs was performed using SSR markers. The PCR reactions were performed with 15 μl final volume containing 50 ng (2 µl) of genomic DNA, 1.5 µl of 10X buffer containing 25mM MgCl2, 1.5 µl of 2.5 mM dNTP mix, 1 µl each of forward and reverse primers (5 mM), and 1U of Taq polymerase. The

This essay based on the principle of real time PCR which uses CYBR green dye that combines to any double stand DNA. This process included two maim steps. The first step was designing of HSV-1 primer and /or HSV-2 primer (we have chosen only HSV-1). BioEdit software has been used to edit the nuclide sequences where necessary. After that the primer has been ordered. The second step was in the laboratory which included applying the HSV-1 primers to real time PCR protocol. The final results of this protocols aim to demonstrate the quality of HSV-1 primer that we have designed by interpretation three criteria. These are specificity, efficiency and

Similarly, according to the published protocol specific primers were used for the detection of CHIKV (CHIKF — 5′-ACCGGCGTCTACCCATTCATGT-3′, nt10237-10258 and CHIKR — 5′-GGGCGGGTAGTCCATGTTGTAGA-3′, nt10544-10566) targeting a 325 base region in the cDNA encoding E1 protein of CHIKV strain 653496 (Accession # AY424803) amplified the products using thermal cycler (Master cycler, Eppendorf, Germany) (Myers and Carey, 1967; Khan et al., 2002). The PCR products from the above reactions were analyzed on 2% agarose gel in the figure below. The sequences obtained were blasted against the NCBI database to ascertain the amplification of specific viral sequences.

The lignocellulosic hydrolysate will be prepared by the National Institute of Molecular Biology and Biotechnology (BIOTECH, UPLB) from sweet sorghum bagasse subjected to pretreatment followed by sulfuric acid (0.75 wt% and pH 1.1) hydrolysis at 100∘C.

Pepino mosaic virus, also called PepMV is a relatively new virus found in plants. It has become a wildly and fast spread virus. This virus is wide throughout and especially in tomato production. Thus pepino mosaic virus has become a significant commercial and agronomical problem.

The viruses of this genus are only transmitted by insect vectors. The Rice tungro spherical virus and Maize chlorotic dwarf virus are transmitted by leaf hoppers while dandelion yellow mosaic virus is transmitted by aphids. Transmission is also semipersistant, non-circulative and non-propagative and requires helper protein. The rice spherical

Bag, S., S. I. Rondon, K. L. Druffel, D. G. Riley, and Pappu, H. R. (2014). Seasonal dynamics of thrips (Thrips tabaci) (Thysanoptera: Thripidae) transmitters of Iris yellow spot virus: a serious viral pathogen of onion bulb and seed crops. J. Econ. Entomol. 107: 75-82.

M. Sc. in Microbiology, Division of Microbiology, Faculty of Agriculture, Annamalai University, Annamalai Nagar, Tamil Nadu

Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Res. Center (ARC), Giza, Egypt.

The present investigation was carried out at the experimental farm and tissue culture laboratory of Genetics Department, Faculty of Agriculture, Zagazig University and biotechnology laboratory of Botany Department, NRC Egypt during the period 2011-2015.