# A Brief Note On Concentration Of Protein And Number Of Recycles Essay

977 WordsMay 7, 20154 Pages
4. RESULTS AND DISCUSSION The Table 1 shows the main results obtained by Method 1 (Full recycle with addition of protein). As it is possible to see, this experiment was carried out in duplicate. In the first run, it was made 3 recycles, and during the second run, it was carried out 2 recycles. The data of protein concentration in function of the number of recycles was plotted on a graph. Table 1: Results obtained by the full recycle with addition of protein Run Recycle Total protein used (%) Protein added (g) Protein used per recycle (%) Concentration (% w/v) 1 0 32.0 20.0 32.0 3.1 1 1 44.4 7.2 12.4 4.1 1 2 62.8 9.6 18.4 3.6 1 3 95.8 5.3 33.0 2.4 2 0 44.4 24.42 44.4 2.1 2 1 74.6 12.08 30.2 3.4 2 2 92.7 4.53 18.1 3.7 Figure 9: Concentration of protein versus number of recycles – Method 1 Analyzing the data of concentration, it was obtained two opposite answers. In the first experiment, the concentration decreased along the recycles, while in the second experiment, the concentration raised. So, these results are not totally conclusive. More experiments should be carried out in order to check the repeatability of the data. However, it is expected the second run represents the real behavior of the process. During the reutilization of the solution, the process efficiency supposes to be lower as it is made new recycles due to the low quality of the remaining protein solution. If the process efficiency is reducing, it means that the concentration is increasing. Regarding