A Case Study Of Drosophila Melanogaster Stocks

Pillbugs and sowbugs are terrestrial isopods that belong to the order Isopoda and the arthropod class crustacean. According to the Current Topics in Microbiology and Immunology, the terrestrial isopods have evolved from the marine forms. However despite the fact that they seek moist environments they must also avoid an overly moist environment (Journal of Insect Science, 2008). The two different species resemble one another phenotypically. However, pillbugs are a dark gray and the sow bugs are a light gray with posterior projections. Pillbugs also curl into a ball when they feel endangered and the sow bugs do not. The pill bugs used in this experiment were female. Isopods primarily live in dark, damp habitats in order to prevent dehydration (Isopod Behavior Lab). The purpose of this experiment was to determine the type of environment isopods prefer. First we tested to see if the isopods preferred a dry or moist environment and then we tested to see if they preferred the potting soil or the leaf litter environment. For the observation of isopods in response to moisture experiment it was said that each of the two different species would prefer a different environment. For part two, Observation of Isopods in response to other variables, it was hypothesized that the Isopods would prefer the potting soil or the leaf litter environment.

Oils, smoke, and pesticides often have different chemicals. When these substances are dumped into the environment by factories and other corporations, they pollute the environment. The chemicals in these substances can often reside in an area for extended periods of time, with even longer lasting side effects (Martinez-Haro, et. al., 2013). This means that if the soil of a pillbug habitat is contaminated to become more or less acidic by these chemicals, the effects on that population of pillbugs may be permanent, and should be investigated to see their long term effect. Lastly, concrete, which is often poured onto the ground and hardened by humans in cities, can cause a potentially harmful change in pH. Concrete has a pH of about 9. Over time, concrete may erode into the ecosystem of a pillbug, increasing the normal pH of it’s habitat. This may harm the organisms in that soil, including pillbugs (Kawahara, et. al., 2016). Pillbugs (Armadillidium vulgare) are a terrestrial isopod that live under rotting wood and fallen leaves, in a habitat whose water content is greater than 10% (Smigel & Gibbs,

Certain fish, such as blue gill and bass, are highly affected by this chemical. Bees and other more needed insects are also negatively impacted by the use of malathion.

Daphnia, also known as water fleas, are small crustaceans about 1mm-5mm long and are part of the freshwater zooplankton (Ebert 2005, Hutchinson 2005 & Clifford 1991). Daphnia can be found in most fresh water habitats such as freshwater springs, ponds and reservoirs and are the predominant food for planktivorous fish. Dapnia are ‘filter feeders’ meaning they feed on small particles suspended in the water which can include algae. It has been found that daphnia tend to migrate to the upper parts of the water at night and return to the lower parts of the water in the day to hide from predators (Ebert 2005) (Hutchinson 2005). Daphnia can reproduce through sexual reproduction and also asexual

Firstly, eggs were collected from the eyes of Scomberomorus niphonius and disinfected in 1% sodium sulfite solution for 3 min, and subsequently in 3% Lysol brand disinfectant for 5 min. The disinfected eggs were then transferred to sterile vials to clone. Secondly, third stage larvae of Lucilia sericata were selected to be placed in 3.5% formalin for 5 min, 2% hydrogen peroxide solution for 3 min, and then 5% dilute hydrochloric acid solution for 5 min. After the two-step disinfection, the larvae remined vigorous. A hundred randomly selected larvae were proven to be aseptic by bacterial culture test.

The goal of this study was to induce a deletion in the DMAP1 gene on chromosome two in Drosophila melanogaster through P-element mobilization. The DMAP1 gene may be an essential gene, however not much is known about it. We attempted to uncover the function of DMAP1 by creating a series of genetic crosses and selecting for brown-eyed non-stubble male flies that may have the deletion. To test whether these flies had the deletion, we produced PCR products and ran them on an agarose gel, which resulted as inconclusive. We created a balanced stock of flies homozygous for the deletion to see if the

The important procedures of this experiment was to observe the pill bugs and determine whether they prefer a certain environment over another. The results and data of the lab show and prove that the isopods prefered the room temperature environment over the warm environment. The hypothesis is accepted because it states that if the isopods were given a choice of a room temperature environment or a warm environment, the isopods will choose the room temperature environment, and the isopods chose the room temperature environment. The control of this experiment was the room temperature chamber.

This experiment was performed using the crustacean Armadillidium vulgare, also known as the pillbug. Pillbugs mate in the spring, and it takes several weeks for their fertilized eggs to hatch. After hatching the offspring spend an additional six weeks in a pouch under the female pillbug. Pillbugs eat dead and decaying organic matter, so they are often found in dark damp places. Another reason they like damp places is because they have gills that must be kept moist. The gills are located on the thorax, which is covered by large exoskeleton plates. The thorax is also where the pillbug’s seven pairs of legs are located. The pillbug also has the nickname “roly-poly,” this is because the pillbug has a defensive mechanism where it rolls into a tight ball, using the large exoskeleton plates of its thorax to protect its head and underside.

Studies of Daphnia presented information which elluded to the idea that Daphnia would react to environmental pollutants within a relatively short timeframe; therefore, a lab was constructed to find the effects of copper sulfate in a Daphnia’s system. Before beginning, the hypothesis was gathered: if the concentrations of copper sulfate in the water are high, then the Daphnia would be afflicted with symptoms associated with physical decline. Daphnia, known as water fleas, are small crustaceans who get their common name from their jerky movements. The organisms reside within lakes and ponds often in limestone-based areas found all over the world. Daphnia consume algae, specifically the free-living green type, yeasts, and bacteria; therefore,

They moved more in this trial. The pill bugs that started on the unscented side moved to the scented side. When one of the pill bugs in the unscented side started moving around, 3 more followed and they went to the scented side for the last trial.

Install a pheromone trap in your pantry. This will draw any lingering moths away from your food and trap them to prevent another infestation. The presence or lack of moths in your pheromone trap can also be used as a tool to monitor for moths. If your trap is empty, you'll know for certain that you have rid yourself of these pests.

The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related

METHODS: In this experiment, the instructor provided us with 30 ebony individuals and 20 wild type individuals. In order to get an exact amount of each type, we anesthetized the flies and counted them off by gently using a fine point paint brush. Then all 50 Drosophila were put into a population cage which had a lid that had six holes for the centrifuge tubes. Two food tubes and four clean, empty tubes were added on the first day. Each food tube consisted of half a cup full of food mixed with 6-7 milliliters of water. This was the fly medium. The food should turn blue once the water is added. Each tube was labeled with a number and with the date. Every two to three days we added one more food tube until all 6 tubes contained the fly medium. After all 6 tubes were filled, the following days after we exchanged the first food tube with a new food tube. At the end of the experiment, we fed the flies with a total of 8 food tubes. Then the flies were anesthetized, again. At the end of this four week lab, the number of living ebony and wild

Bt [proteins] have been tested against a wide variety of terrestrial and aquatic invertebrates including earthworms, collembola, daphnids, insect predators and parasites, spiders, and honeybees and have been shown to have a high degree of safety for these non-target organisms. In most cases, no adverse effects were observed even though test populations were exposed to levels of toxin in excess of 500-1,000-fold concentrations than they would be expected to encounter under field conditions.

There was no significant (p<0.05) difference between the initial weights of the lugworms in the 4 sample groups, avoiding any bias in treatments. As Figure 4 shows each sample group showed a large increase in weight after exposure to the diluted salinities with the lugworms that were placed in full strength seawater not showing an increase of more than 1.7%.