A Experiment On Hydrogen Peroxide

The more acidic a substance is the less oxygen it will produce when going through a chemical reaction. During the Lab “How Do Changes in pH Levels Affect Enzymes Activity”, the researcher conducted an experiment to test the effects that an acidic, neutral, and a base substance will have when combine it with hydrogen peroxide. The data table shows that HCL (acidic substance) barley produced any oxygen at all when it was combining with Hydrogen Peroxide. The pH level for HCL was 2.5; this level indicates that the substance was very acidic. When the H2O and NaOH were tested they produced more bubbles than HCL. NaoH produced a little more bubbles than HCL. The pH that NaoH produced was a 9, which is a base. H2O produced more bubbles than both substances;

What is the effect of the concentration of a hydrogen peroxide solution on catalase activity?

The topic of this lab is on biochemistry.This experiment was conducted to show how cells prevent the build of hydrogen peroxide in tissues. My group consisted of Lekha, Ruth, and Jason. There were used two different concentrations of hydrogen peroxide through this experiment , 1.5% and 3%. By testing two different types it is easier to understand how the H2O2 and catalase react with one another. To do this both the yeast, which was our catalase, and H2O2 were mixed together in a beaker. Each concentration was tested out twice for more accurate results . 1.5% concentrated H2O2 had an average reaction rate of 10.5 seconds while 3% concentrated H2O2 had an average reaction rate of 7.5 seconds. From this experiment we learned that by increasing the concentration of H2O2 and chemically combining it with a catalase it will speed up the reaction. Enzymes speed up chemical reactions . The independent variable in this experiment was the concentration of the H2O2. Some key vocabulary words are Catalase, enzyme, hydrogen peroxide ( H2O2), and concentration.

The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level).

Weight 30 dry pre-82 pennies which get 89.77g, using 30ml initial volume measuring the volume of 30 pennies, record the data 10.0ml. Using equation Density= Mass/Volume, get the density of the pre-82 pennies is 8.98g/ml. Then calculate the error%=0.10%, and the deviation%=1.29%.

There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.

We hypothesized that a medium pH buffer added to the hydrogen peroxide an peroxidase reaction would be the best condition for the enzyme activity due to it being the more neutral than the high, being basic, and low, being acidic, pH.

The differences for the rates of reaction between the liver and potato are accounted for because liver contains more catalase enzymes than potatoes. This is because the liver is responsible for ridding toxins out of the body and as a result needs more catalase enzymes to do so; explaining the bigger reaction it had with the hydrogen peroxide.

Andra Larade Bio 30 B7 11/20/15 Liver Enzyme Lab The Effect of pH and Temperature on the Efficiency of Catalase Abstract: In order to determine how pH and temperature affect the liver enzyme catalase, an experiment was conducted. Catalase breaks apart hydrogen peroxide producing water and oxygen.

The purpose of this laboratory experiment is to explore the effects of pH has on a reaction rate. The reaction studied was the breakdown of hydrogen peroxide catalyzed by enzyme peroxidase. Peroxidase is a large protein containing heme co factors in its active site. Four trials were ran at pH levels of 3, 5, 7, and 9. I hypothesized that the reaction would run very quickly at a pH 7, since that is the normal condition of cells where peroxidase is found.

The research and observations of this lab primarily focused on the enzyme activity of the enzyme Peroxidase. Peroxidase is a large protein and is composed of more than three hundred amino acids. The enzyme was selected as it is easy to experiment with and effectively showcases the effects of varying independent variables, such as pH and temperature. Peroxidase catalyzes the decomposition reaction of the chemical Hydrogen Peroxide ( H2 O2 ) into water and an electron donating molecule, which stands for R in the written chemical equation. ( The equation is displayed below:

Observing how the enzyme catalase found in chicken and beef livers breaks down hydrogen peroxide at varying pH levels and temperatures.

Catalase is an enzyme needed to break down hazardous hydrogen peroxide into nonhazardous oxygen and water. This is needed for organisms as the reaction rate of the decomposition of hydrogen peroxide is too slow to protect cells against the oxidation of hydrogen peroxide. The purpose of this experiment is to test the concentration of hydrogen peroxide on catalase and variation in temperature of catalase to find the optimal values that will produce the highest rate of products. It was hypothesized that as the concentration of substrates increases, the reaction rate will as well. The null hypothesis for this is that the substrate concentration will have no effect on the reaction rate. For the varying temperature of catalase, it was hypothesized that as the temperature increases, the reaction rate will increase as well until the enzyme is denatured. The null hypothesis for this is that varying temperature will have no effect on the reaction rate. The experiment was completed by using a 10mL graduated cylinder filled with water that collected oxygen gas that was produced by the reaction. From this experiment, one can see that as the amount of hydrogen peroxide concentration increases, the rate of the reaction increases as well. The results support this as the average reaction rate starts at 0.00 mL O2/min for 0.0% hydrogen peroxide and raises to 38.17 mL O2/min for 0.8% hydrogen peroxide. As the temperature of catalase was increased, rate of reaction continued to

Each cube was placed in separate Hydrogen Peroxide Mixtures. The resulting oxygen produced from the decomposition was recorded in ml, from the downward displacement of water (see fig 1.1). Results were taken every minute for 20 minutes.

Hydrogen peroxide is a toxic byproduct of cellular functions. To maintain hydrogen peroxide levels the catalase enzyme deconstructs hydrogen peroxide and reconstructs the reactants into oxygen gas and water. The catalase enzyme is found inside cells of most plants and animals. Regulating the levels of hydrogen peroxide is crucial in homeostasis and analyzing it’s optimal conditions for performance is just as important. To understand the optimal environment for this enzyme, they are put into different environments based off protein activity (enzymes are proteins). Catalase samples will be put into different hydrogen peroxide environments based off pH and temperature. The more active the enzyme, the more oxygen and water it will produce. Enzyme activity can be seen through the release of oxygen in the hydrogen peroxide. Since oxygen cannot be accurately measured, the data will consist of the longevity of the reaction in different environments. If the pH is higher than 7, then the reaction rate will increase due to the ample amount of hydrogen ions in the hydrogen peroxide. However the pH level cannot be higher than 10 or else there will be too many hydrogen atoms in the peroxide for the enzyme to be able to deconstruct them. If the temperature is increased, then the reaction rate will increase due to the ample amount of energy and movement in the hydrogen peroxide and enzyme.