A Fluorescent Mirna Sensor For The Study Of Exrna And Drug Delivery

Expression of mir124 and 184 decreased significantly in old R2 neurons. These results suggest that aging of neurons is associated with specific changes in expression of miRNAs. Furthermore, these results are consistent with our previous finding that aging is associated with neuron specific changes in gene expression [30].

MicroRNAs (miRNA) are small noncoding RNA, usually 17-25 nucleotides long that are able to bind complementary sequences of target messenger RNA (mRNA) and to induce both their degradation and translational repression (Fortunato, et al 2014). They are one of the most significant classes of non-coding RNA molecules (eg. small interfering RNA (siRNA) and ribozymes) that act within the cell. MiRNAs are also evolutionary conserved in different species from plants to humans and are encoded by their respective genes (Bader, 2012).

Introduction: Mutation within cell populations are seldom.1 The mutS gene in E.coli takes part in the repair and recombination of DNA.2 When mutS was deleted from E.coli in a previous study, the mutation rate increased when compared to the wild type strain.2 Rifampicin is known for its inhibition of RNA Polymerase production.3 Without RNA Polymerase, RNA is incapable of production and thus protein synthesis ceases within the cell, resulting in cell death. We hypothesize that since mutS repairs recombinant DNA within E. coli, the deletion of the mutS gene will increase the mutation rate of E. coli.

MiR-21 has been found to be over expressed in 20 pancreatic carcinoma tissues and cell lines compared to normal tissue or cell lines. (15)

Recent reports indicate the potential role of miRNAs as both therapeutic targets and biomarkers in IPF (5). MiRNAs are small (approximately 22 nucleotides in length) and stable non-coding molecules, which can regulate gene expression (6). They are important regulators of physiological processes, such as cellular proliferation leading to lung tissue repair. The miR200 family is functionally involved in canonical pathways of immune response and cell-cell communication. Here we propose novel studies using miR200b and miR200c in IPF compared to controls, first, to determine their function in the niche composed of ATII cells and fibroblasts, second, analyze their potential to stimulate ATII cell proliferation and transdifferentation to alveolar type I cells, third to analyze their capacity to inhibit the growth of fibroblasts; and fourth, to use them as early biomarkers of IPF development. The rationale for using 200miRNA family members as curing IPF drug is based on their dysregulated expression observed in IPF (7). Moreover, our

Biomarkers are natural indicators in the body that respond noticeably to the presence of disease. By measuring the responses of biomarkers, oncologists detect the cancerous cells before recommending expensive procedures, rather than immediately resorting to these procedures. One of these biomarkers that responds to the presence of cancerous cells is microRNA. MicroRNAs are molecules that are involved in the cell life cycle and therefore the progression of cancerous cells. A study in cancer patients was conducted to compare the accuracy of screening with microRNAs to screening with LDCT. Observing microRNAs did not prove to be as accurate at identifying cancer as LDCT, but since using microRNAs is a less expensive process, it can identify patients that should undergo LCDT testing. Screening with microRNA testing initially would decrease the number of patients paying for LCDT testing.

Rhizopus delemar is an emerging fungal pathogen that causes devastating mucormycosis in individuals with a weakened immune system. The symptoms of mucormycosis are non-specific and definitive diagnosis requires time-consuming and often insensitive direct examination of specimens and cultures of the organisms. Recently, extracellular vesicles (EVs) with encapsulated RNAs (exRNAs), have emerged as an important cell-cell communication conduit that holds great promise not only in diagnoses but also treatments of cancer and infectious diseases. We have performed deep-sequencing, profiling and annotation of extracellular small non-coding ribonucleic acids (ex-sRNAs) isolated from two clinical strains of R. delemar. Approximately 3.3 and 3.2

Medsaic developed new platform technology, now known as DotScanTM, which is a solid-phase cell-capture assay using an antibody mircoarray to immunophenotype cancer cells[2], as well as

microRNAs (miRNAs) are a short ( about 22-26 nt length ) non coding RNA that involved in regulating gene expression at the level of post-transcription or translation and apparently control a broad range of processes of multicellular organisms including cellular differentiation, proliferation and apoptosis via targeting mRNA and cause their degradation or protein translation inhibition. miRNA transcribed from long pri-miRNA in the nucleus then processed by Dicer enzyme in the cytoplasm to form mature miRNA that incorporate in RNA induced silencing complex (RISC) to enhance their functions.

miRNA shuttles offer stable gene silencing, flexibility in design, and effective delivery to target tissues with the help of AAV vectors, making their use an option worth exploring for treatment of various neuromuscular

RNA interference pathways play an integral role in the silencing of gene expressions, predominately by destroying specific targeted molecules of messenger RNA (mRNA). Messenger RNA are single- stranded RNA molecules which move from the nucleus of the cell to the cytoplasm, while sending and receiving genetic information from DNA via ribosomes, to where the specific amino acid sequence of the protein involved in gene expression is located, as the article, “Messenger RNA - Glossary Entry” explains. The RNA interference pathway (RNAi) breaks double- stranded (dsRNA) molecules which match a specified targeted gene into short single- stranded RNA (ssRNA), which triggers the deterioration of the mRNA in its corresponding sequence. According to Cartel,

Surprising, the target of both the miRNAs has the same target and functions in a similar way. Further study exposed that the EBV-infected cells escaped the apoptosis mechanism due to the expression of miR-155. This shows the potential of virus that controls the miRNA expression for its survival benefit (14).

In this article, we aimed to research the diagnostic value of peripheral blood and tissue miR-193a-5p. And we also tend to illuminate the underlying molecular modulatory mechanism of miR-193a-5p in lung cancer. Hereby, we comprehensively retrieve the eligible microarrays and performed meta-analysis to explore the clinical diagnostic significance. Then we adopted bioinformatics analysis to lucubrate the potential molecular mechanism. We collected predicted and validated target genes of miR-193a-5p and further acquired the overlapping genes. GO, KEGG and PPI network analysis were further performed for the overlapping genes. We hope what we obtained might offer references for future researches.

PM331 Essay: Exosomes, their cell biology, normal function in the cell, and role in disease.

Aim-3: To validate the automated mRNA FISH assay of ARv7, ETS gene fusion and PTEN on DeNovo’s JETTA™ microfluidic chip, Jetta 400 sample preparation system and Vanguard image processing system. Demonstrate the entire system capable of automated single cell mRNA FISH on CTCs using blood samples from patients with metastatic prostate cancer.