A Laboratory Report On Spectrophotometry

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A Laboratory Report on Spectrophotometry
Aliya Shah 20th October, 2016
Isaac Newton’s discovery that white light can be split into a spectrum of colours forms the fundamental basis of spectroscopy. The spectrophotometer was discovered in the early 1800’s by a scientist named Joseph Von Fraunhofer. Spectrophotometers are used by students and professionals in a variety of fields including research and other scientific disciplines. Spectrophotometers function by allowing us to pass different wavelengths of light from the electromagnetic spectrum through any sample we intend to investigate. In our experiment, we used the spectrophotometer to determine the concentration of cytochrome C. Cytochrome C can be extracted from animal mitochondria where it is attached to an organic haem group and acts as an electron carrier during metabolism. A basic understanding of a spectrophotometer is useful when carrying out this practical.
In a spectrophotometer light is converted into pockets known as photons and this energy is transferred to electrons when they collide. Photons can then be promoted to a higher energy level if the energy absorbed is equal to the energy required for the photon to be promoted. Spectrophotometers are comprised of a light source usually from a tungsten lamp which passes through an entrance slit, monochromator, exit slit, cuvette, detector and
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