A Measurement Of Around 0.5 µl, For Each Compound, Was

1585 WordsFeb 8, 20177 Pages
A measurement of around 0.5 µL, for each compound, was inserted into a gas chromatography in such a way, a syringe holding the compound was pushed down into a column and injection occurred to then start the machine in separating the compound. A computer was right beside it, indicating the graph of multiple peaks and later, after a duration of 4 minutes, a graph was printed out and obtained to then analyze the retention times and percent composition. “The percent composition was found by inserting the following values into the following equation, Mole % A = (area of Peak / A total area)×100% acquiring our desired values shown in the table above” (Z. 2016). Discussion Odor Analyses: Beginning the experiment, a test of determining which…show more content…
However, instead of calculating the refractive index, the following values were obtained by inserting each compound into a refractometer and carefully following the procedure to then retrieve the value shown through the lens. The following shows the compound along with its refractive index: 1.4601 for spearmint, 14554 for caraway oil, 1.4638 for S-(+)-Carvone, 1.4661 for R-(-)-Carvone, and 1.4602 for limonene. For the two types of Carvones used while determining their refractive index, comparing R-(-)-Carvone with a value of 1.4661 and S-(+)-Carvone with 1.4638 we do see a difference of 0.0027 among the values, but all in all, being quite similar from a standpoint and a bit more different from the other compounds like spearmint. However, towards their reference values at 20℃, a difference is clearly seen, to where for R-(-)-Carvone, comparing the refractive index we got at 1.4661 to the literature value of 1.497 (R-(−)-Carvone 2017) , with a difference of almost 0.03. The same goes for S-(+)-Carvone, with the refractive index of 1.4638 to the “literature value of the same value, 1.497” (S-(+)-Carvone 2017), with a difference of 0.035; error may have occurred here when looking at the following compounds through the refractometer either through incorrectly aligning the adjuster to the correct color sequence or improperly adjusting the “X” symbol to which no black spots can be

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