A Research Group On The Large Scale Experiment

902 WordsApr 27, 20154 Pages
Results For this experiment our research group produced our own taq. Being able to do this saved our lab a lot of money for such a large-scale experiment. After the moonshine taq was made and amplified using a PCR, we ran a test gel to determine if the taq was functioning and could replicate DNA. Figure 1 shows that the taq was capable of replication. Here we also observe that each band traveled 500 bp, meaning that the mass of the samples was roughly 97ng. We tested both heat-treated taq, and non heat-treated taq to determine if one or the other yielded better results. We did not see a difference in the results between the heat-treated and the non heat-treated taq, which suggests that the heat-treating step is not necessary. We also compared the two moonshine taqs that we made to commercially prepared taq (shown in lanes 1 and 2). Our taq was very comparable to the commercial taq. Next we turned regular E.coli cells, into chemically competent cells. This means that the cells were now able to act a host cel1 and accept the vector. Through transformation, we were then able to insert the pCS2 + GFP into the E.coli cells.. We then isolated the pCS2 + GFP from the chemically competent E.coli cells that we previously made, using a miniprep protocol. To observe whether or not we successfully isolated the pCS2 + GFP, we ran a gel. Figure 2 shows us that each sample contained the pCS2 +GFP after the miniprep was preformed. This supports that the pCS2 + GFP were all successfully
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