A Research Group On The Large Scale Experiment

E. coli HB101 was transformed with pGLO plasmid then grown on media containing ampicillin and/or arabinose and on medium containing neither (Brown, 2011). This is done for selection of transformed cells since not all cells are expected to take up the plasmid (Brown, 2011). We also expect roughly the same CFU on any plate(s) receiving samples from the same microcentrifuge tube, since they are getting the exact same

The experimental part of the lab consists of setting up the materials needed. A sample of E.coli and a solution of calcium chloride are first obtained and placed in different test tubes. 630µL of Calcium Chloride (CaCl2) are then removed from the test tube and inserted into the test tube containing E.coli cells (Alberte et al., 2012). The newly formed substance of Calcium Chloride and E.coli is then mixed and incubated in ice for 10 minutes, making the cells more competent. Two test tubes are obtained and labeled; the first test tube is labeled with pUC18 and the second one with “Lux” to represent the plasmids being used. These two test tubes are then incubated in ice. 3µl of the set plasmid are added to each of the two test tubes. The test tubes are tapped to guarantee the cells are well

The plasmid pGLO contains an antibiotic-resistance gene, ampR, and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus, transformed E. coli cells containing ampicillin-resistance plasmids can easily be selected simply growing the bacteria in the presence of ampicillin-only the transformed cells survive. The ara control region regulates GFP expression by the addition of arabinose, so the GFP gene can be turned on and

Would the GFP gene in a pGLO transformed E. coli be activated by a sugar other than arabinose?

How does the addition of pGLO plasmid to a solution containing E. coli bacteria affect the growth and characteristics of the bacteria? Genetic transformation is the incorporation of foreign DNA into an organism to potentially change the organism’s trait. Plasmids are small circular DNA that replicate separately from the bacterial chromosome. In nature, these plasmids can be transferred between bacteria allowing for the sharing of beneficial genes. Due to this characteristic, plasmids allow for genetic manipulation and can be moved between bacteria easily. The pGLO plasmid utilized in this experiment encodes the gene for Green Fluorescent Protein (GFP), which under the right conditions can produce a glow. The gene regulation system present in the pGLO plasmid requires

In the future, this experiment could improved or expanded. One area of improvement could be to do this experiment several times and then averaged together or compare the results. This leaves less room for error and could make the data more accurate and precise.

The Great Experiment was a period during the 18th century, where job expansion increased so there was less unemployment in the United States. Experimentation occurred from the mid 1700s until 1787. The Great Experiment recognized that individual rights are derived from a Creator. Power was restricted through The Constitution, and it carefully divided, balanced, and separated the powers of the government and organized them through a sytem of checks and balances. The Great Experiment was the exact opposite of the period that occurred in the 1930s, The Great Depression, when jobs were limited and the stock market crashed.

Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify recombinant GFP using HIC. Once the protein is purified, it may be analyzed using polysaccharide gel electrophoresis (PAGE).

The pGLO plasmid is being introduced. This plasmid contains genes for GFP

During World War Two, a handful of experiments were conducted on the prisoners of the concentration camps by German physicians. They were painful, and often deadly. Concentration camp prisoners were experimented on without their consent.

This week will be the Experimental Design Discussion lab that will also act as an introduction to the Beetle Behavior lab over the next few weeks. The students will go over proper experimental procedures/designs because the beetle lab is more free-thinking than other previous labs. They get to establish their own hypotheses and procedures as long as it correlates with beetle behavior of course. It would be beneficial for you all to look over the Romero article again as it has valuable information about beetle behavior that could help the students in formulating a workable, observable experiment. The students will be observing the beetles in the big glass dishes that will be set up at each station. Make sure they properly handle the beetles

Methodology: PRP was produced by precipitation using ethanol and then it was treated with cetavlon and hydroxylapatite. To produce recombinant PE (rPE), pe gene was cloned in pBAD-gIIIA system and expressed in E.coli TOP10. After purification, rPE was complexed with PRP. BALB/c mice groups and then they were immunized with rPE+PRP complex, PRP and rPE alone. Antibody titer against antigens were evaluated in serum samples that were collected at days 0, 14 and 28 by ELISA method.

There were many different research methods to choose from I thought the experiment method was the most effective. First off to make experimental questions you need explanation,require previous knowledge and the question is testable. Common questions used for the experimental method are cause and effect questions. What the researcher is trying to determine is what the patterns and effects were after the modifications. Finally the experimental method was the best approach in my opinion because essentially you have proof, you have data showing you exactly what happened to whatever it is you put thru experiment.

For this assignment I chose to analyze the article “Racial and Ethnic bias in decisions to shoot seen through a stronger lens: experimental results from high-fidelity laboratory simulations” from Journal of Experimental Criminology to identify the elements of research design that was used in this empirical research. The stated research question or hypothesis contained within this particular study was, “Does subconscious bias against a particular racial or ethnic group predict decisions to shoot in a laboratory setting” (James, Klinger, Vila, 2016, p. 324). This research questions was formulated in light of many of the deadly force situations occurring within American society were there seems to be marked racial disparity with African

Experimental research is sometimes done with risk of harming the subjects under study. In a research study, participants could be physically and emotionally harmed. As nurses, we are advocates for our patients. Therefore, our goal is to protect them from any harm and discomfort when they participate in experimental treatment. Many dilemmas arise when trying to determine the level where an experimental treatment becomes harmful. In the past, researchers conducted unethical experiments without the subjects’ informed consent. They conducted experiments on human subjects knowing the high potential for harm. Participants received harmful substances without their knowledge and did not receive treatment (Grove, Gray, & Burns, 2015). Researchers coerced, misinformed, and forced human subjects to participate in experimental research. Groves et al. (2015) reported few events from the past that displayed a breach of the participants’ privacy, confidentiality, and freedom. Rodriguez and Garcia (2013) also reported a study where American scientists infected a specific population in Guatemala with sexually transmitted diseases such as syphilis and gonorrhea. American researchers conducted unethical experiments on human subjects without proper informed consent and clearly caused harm to mentally and socially vulnerable participants. The American researchers carefully selected a segment of the Guatemalan population based on poverty level and mental capacity. They did not inform the