A Research Study On Cultured And Handled At 20. C Using Standard Methods ( Brenner ) Essay

This lab investigated the central dogma of molecular biology by studying the expression of jellyfish Green Fluorescent Protein in E. coli (Mardigan, 2011). After transforming E. coli with the GFP gene under the arabinose operon promoter, GFP expression was analyzed with and without arabinose. Induction was seen only in media with arabinose. To further study GFP protein and structure, an SDS-Page or gel electrophoresis was conducted where proteins were charged to allow them to move in an electrical field in terms of their size. To further study the role of the arabinose operon in GFP expression an overnight culture was done where the plate was frozen and incubated to induce optimal protein folding. The GFP was then purified. Centrifugation, use of buffers, and a column was used to separate the GFP and bacterial cells.

Part 3 of this experiment calls to isolate the DNA from C. Elegans. To do so, we start by labeling three PCR tubes with the group number. One of the tubes is labeled “W” for wild type, one is labeled “R” for

The phrase “Collision of Cultures” is an accurate assessment of the early relationship between the New World and the Old world. Each side of the cultural divide viewed the other through their own preconceptions that originated from their own standards and customs. The biological and social exchange of the two cultures favored the Europeans at the expense of the Native American Indians.

This lab is about moving genes from one thing to another using plasmids. Plasmid has the ability to replicate, so it replicates independently, and separately from the chromosomal DNA. Plasmid are one or more small piece of DNA and they enter cells as a double strand DNA. When they enter the cell as a doubke strand they do not invade he chromosomal DNA. We will also transform bacteria into GFP which is mainly from the jelly fish Aequorea Victoria. The GFP causes the the jelly fish to fluorescent and glow in the dark. After the transformation, bacteria starts to make the GFP which causes them to glow a green color under a ultraviolet light.

The GFP gene is normally isolated from the jellyfish Aequorea victoria, allowing it to fluoresce with the absorption of ultraviolet light (Goodsell, 2003). This gene is inserted into a region of a plasmid to be regulated by an operon (Brown, 2011). In the plasmid, GFP is normally repressed but when arabinose is present, it will induce the gene to make enzymes able to digest the arabinose, and once there is no more arabinose,

The purpose of the PGLO lab was to be able to perform a procedure known as a genetic transformation. We used a procedure to transform bacteria with the gene that codes for a Green Fluorescent Protein (GFP). The actual source of the GFP gene that we used in this complicated experiment is the bioluminescent jellyfish Aequorea victoria. This protein causes the jellyfish to glow under a UV light that was provided in the dark. After the transformation procedure, the bacteria showed their newly acquired gene from a jellyfish and produced the fluorescent protein, which as a result, causes it to glow. If the bacteria glowed in the dark, that was the initial sign that the experiment was successful.

In this experiment we were meant to observe the transferring of DNA. There are many ways in which DNA can be transferred into an organism, for example; transformation, transduction, and conjugation. In our experiment we used

In the pGLO Bacterial Transformation lab, Escherichia coli is transformed with a gene encoding green fluorescent protein by inserting a plasmid containing the GFP gene, beta-lactamase, and arabinose into the bacterium. Successfully transformed bacteria will grow in the presence of ampicillin and glow a bright green color under ultraviolet light. The sugar arabinose is responsible for switching on the GFP gene in the transformed cells, without it, the gene will not be expressed.

The GFP gene allows for the bacteria to have the information to produce the fluorescent color. However, this is not enabled by default. In order for it to be activated, it must be induced by the arabinose. Once arabinose is introduced, the protein can be

Introduction: Transformation is used to introduce a gene coding for a foreign protein into bacteria. Hydrophobic Interaction Chromatography (HIC) is used to purify the foreign protein. Protein gel electrophoresis is used to check and analyze the pure protein. Research scientists use Green Fluorescent Protein (GFP) as a master or tag to learn about the biology of individual cells and multicultural organisms. This lab introduces a rapid method to purify recombinant GFP using HIC. Once the protein is purified, it may be analyzed using polysaccharide gel electrophoresis (PAGE).

Didn’t realize how curiosity plays a crucial part in our critical thinking. TanLe, visuals are always a great addition. TanLe culture does play a huge part in my life. We were not allowed to question anything and obey our elders at all time. I was also, shy and curiosity was not an option. Still today, being 38 with 2 kids and still tend to bite my tongue in efforts not disrespect. I am currently working on it, but it hasn’t been easy. Again thank you Michael and TanLe.

Analysis of DNA from practicals 1 and 2 using the technique of agarose gel electrophoresis and analysis of transfomed E. coli from practical 2 (part B)

The Two Cultures, which was originally a lecture by C.P. Snow, Shows the how Snow sees the split of the Humanities and Sciences and how that it could harm the growth of the world. From what I have gathered Snow believes that the world is run by those who are educated in the humanities and even says that they are “behaving like a state whose power is rapidly declining”. That those who are in command are more worried about keeping afloat than trying to expand. Snow though also show how much he supports the disciplines and wished for the two to begin to work together more. Snow thought that if the sciences and humanities did not work together for progress it could lead to the doom of not only England but the entirety of western civilization.

Nematodes, the marvel of evolution possess incredible ability to survive extreme weather events and hostile environments with a simple body organization. Genome sequences of model nematodes have revealed that many genes and physiological processes in these worms are common to humans, higher animals and plants. Understanding the molecular mechanisms underlying their survival under adverse environments may provide new opportunities in developing novel strategies to minimize/mitigate the impact of global warming. Nematode communities in soil are composed of a variety of trophic and ecological groups which can be directly linked to key ecosystem functions like primary production, primary consumption, secondary consumption, decomposition, etc. Because of these attributes, soil nematode communities serve as an excellent model system for studying impacts of climate change on the belowground productivity. Nematodes are currently being extensively investigated in most studies analyzing response of terrestrial ecosystems to climate change. They are ubiquitous, diverse and the most abundant group of multicellular organisms on the earth. They are represented at more than one trophic level in soil food-webs as they act as primary consumers (herbivores), secondary consumers (bacterivores and fungivores) and tertiary consumers (omnivores, carnivores and predaceous nematodes). They play multiple and contrasting roles in regulating productivity of plant and animal based production systems.

Numerous attempts have been made to isolate, identify and apply effective antagonistic microorganisms to manage RKN, nevertheless the use of nematicides is proved far more effective than biocontrol agents. According to literature, many bacterial isolates showed a significant nematicidal activity against RKN (Zhou et al. 2016; Vagelas and Gowen 2012; Moussa and Zawam 2010; Mendoza et al. 2008) that inhibited egg masses hatching or caused J2 mortality. Therefore, many studies are still being conducted to distinguish and understand effectual biological agents. In the present study, a novel bacterial strain showed a strong inhibitory activity against the M. javanica in dual culture bioassays. Both the whole bacterial cell and cell-free culture