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A Research Study On Genetic Engineering

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Genetic engineering is using molecular biology methods to modify the genetic information of an organism. Its aims include learning about the biology of an organism and generating new or improved commercial products. Plant biotechnology is manipulating plants specifically to improve agricultural needs. GMOs or genetically modified organisms are organisms modified by genetic engineering to express desirable traits. DNA fragments containing said desirable traits and a means of introducing the DNA to the host plants is required for genetic engineering. Some common methods of introducing DNA fragments to plants include the use of viruses, chemicals, some means of physical insertion, or the use of agrobacterium.
Mysore et al aimed to study the mechanism of Agrobacterium transformation, DNA transfer, and the possible limits of genetic engineering. A complementation test of rat5 mutant and Histone H2A (RAT5) showed rat5 mutant is responsible for the mutant phenotype. Transient and stable β-glucuronidase expression and T-DNA levels of wild type and rat5 Arabidopsis plants showed that the rat5 mutant is deficient in T-DNA integration. The researchers also thought rat5 could be haploinsufficient and to test this hypothesis they transformed wild type plants with A. tumefaciens GV3101(pE1553) and found the rat5 mutant does not show dominance in WT plants.1 So, two copies of RAT5 gene are necessary to conduct Agrobacterium-mediated transformation. This paper examines a protein gene (H2A)

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