A Research Study On Nutrient Agar

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The first patient, 18 year old female presented with symptoms of high fever, vomiting, diarrhoea, myalgia, hypotension and signs of septic shock. In order to identify possible bacteria responsible for these symptoms, a vaginal swab was sampled, incubated on agar overnight and stored at 4C, after which they were examined throughout a series of microbiological tests. The initial culturing of the sample on nutrient agar indicated two organisms were present, and hence these were analysed individually. A Gram stain was applied to each bacterium and they were viewed individually under microscope at 1000x oil immersion magnification. This specified both Gram negative diplobacilli, which will be referred to as organism one, and Gram positive staphylococci, which will be referred to as organism two. Organism one demonstrated irregular, flat, wavy, medium sized and cream coloured colony morphology when viewed on agar plate, however limited conclusive data can be obtained from these observations alone. In order to provide further information for diagnosis, additional culturing was conducted on a range of selective and differential media types including Horse Blood, MacConkey, Mannitol Salt and Brilliant chromogenic UTI agar’s. This demonstrated the organism was non-haemolytic, lactose fermenting, as evident through a red colony formation on the MacConkey agar, and unable to survive in high salt concentrations, as indicated through the lack of growth on Mannitol Salt agar. The
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