A Research Study On Protein Guided Injections, And Transcription Activator Like Effector Nucleases ( Zfns )

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The A line in Table 1 is the first group of mice that they bred and B line is the second line is the second group of mice that were bred [4]. Of these two groups 171 mice had more than 10 offspring [4]. Table one shows that without considering the mice with less than 10 offspring that the number of mosaic mice account for a higher percentage of the mice 36% versus 25% [4]. Protein- Guided Injections: Protein guided injections arose out of a need for a more efficient gene editing tool. This led to the creation of Zinc-Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs) [1]. The biggest problem with creating these technologies was finding a restriction endonuclease that didn’t have multiple cleavage sites and whose bind sites were not right next to these cleavage sites [1]. One fit the bill, after some tinkering this restriction endonuclease by the name of FokI was developed to be used in both the ZFNs and TALENs [1]. ZFN’s are chimeric proteins that are bound to “a modular array of Cys2-His2 DNA-binding zinc fingers” that are in turn bound to the FokI [1]. The TALEN’s are Transcription Activator-Like Effector (TALE) proteins that are secreted by bacteria that can control the gene expression of their host cell [1]. These particular bacterial proteins were first observed in plant cells [1]. TALENs assembly is less problematic but harder to synthesize than the ZFNs [1]. They both require formulating new DNA binding proteins for each gene [1].

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