A Research Study On Scar Markers

1635 WordsMar 6, 20157 Pages
SCAR markers are PCR based primers that represent genomic DNA fragments at genetically defined loci, that are identified by PCR amplification using sequence specific oligonuceotide primers (Paran and Michelmore, 1993; Me Dermott et al., 1994). Inception of SCARs involves cloning the amplified products of arbitrary marker techniques and then sequencing the 2 ends of the cloned products. The sequence s therefore used to design specific primer pairs of 15-30 bp which will amplify single major bands of the size similar to that of the cloned fragment. The greater the variation in DNA sequence, the easier it is to generate polymorphic markers. Therefore the plant species used for SCAR development should have sufficient variation for te traits of interest at both the DNA sequence and phenotypic levels. Researchers over the years have employed various non-sequence primed PCR markers to develop SCAR markers for the identification of sex in dioecious species of crop plants. Chromosomes and its sex determining genes have driven genetic control of sex determination in dioecious crops. In dioecious crops a vast variability has been seen in the genetically controlled sex determination and a DNA polymorphism has been mainly attributed, that are directly involved in expression of particular sexual phenotpe (Aryal and Ming, 2013). In dioecious crops either one of the crops will be of great agricultural or commercial interests like Simmondsia chinensis, Hippophae rhamnoides. In Garcinia
Open Document