A Study Of Ja Biosynthesis

In this experiment, the naturally occurring peroxidase is extracted from homogenized turnip (Brassica rapa) pulp (Coleman 2016). Its role in the environment is to remove toxic hydrogen peroxide during metabolic processes where oxygen is used (Coleman 2016). The goal of this experiment is to evaluate the change of absorbency of turnip peroxidase within a metabolic reaction utilizing oxygen. Any change noted is indicative of the peroxidase removing hydrogen peroxide. Within this experiment, the extract will be prepared, the amount of enzyme will be standardized, and the effect of changing the optimal conditions will be observed. If the enzyme concentration is increased then the rate of the reaction decrease. If the pH of solutions used is increased

The role of an enzyme is to catalyse reactions within a cell. The enzyme present in a potato (Solanum Tuberosum) is catechol oxidase. In this experiment, the enzyme activity was tested under different temperature and pH conditions. The objective of this experiment was to determine the ideal conditions under which catechol oxidase catalyses reactions. In order to do this, catechol was catalyzed by catechol oxidase into benzoquinone at diverse temperatures and pH values. The enzyme was exposed to its new environment for 5 minutes before the absorbance of the catechol oxidase was measured at 420 nm using a spectrophotometer. The use of a spectrophotometer was crucial for the collection of data in this experiment. When exposed to hot and cold temperatures, some enzymes were found to denature causing the activity to decrease. Similarly, when the pH was too high or low, then the catechol oxidase enzyme experienced a significant decrease in activity. It can be concluded after completing this experiment that the optimal pH for catechol oxidase is 7 and that the prime temperature is 20º C. Due to the fact that the catechol oxidase was only tested under several different temperatures and pH values, it is always possible to get a more precise result by decreasing the increments between the test values. However, our experiment was able to produce accurate results as to the

Hobart and William Smith Colleges. (2017). Lab 04 BIOL 1510 Lab Manual Plant Defenses F2017 - Biology - StuDocu. Retrieved from https://www.studocu.com/en/document/hobart-and-william-smith-colleges/biology/lecture-notes/lab-04-biol-1510-lab-manual-plant-defenses-f2017/1396233/view

Plant cells have selectively permeable membrane that allows selective substances to penetrate into the cell. Cell membranes are consisting of lipid layer that are made up of phospholipids. (Essays, UK.2013). These phospholipids are amphipathic in nature, which have phospholipids head and tail respectively. Phospholipids heads are hydrophilic and tails

However, for each quad, the fertilizer used for the control was added altogether with the fertilizers being studied. Since the only plant that presented growth was in the control quad, it becomes necessary to explore the effects of excess fertilizers, and furthermore, excess nitrogen, would have in the development of the fast plants. Although Nitrogen is one of the elements most living organisms require, some studies have showed high levels of nitrogen can cause toxic stress to some plants, to the point where growth can be inhibited. (Yu et al.

The process of photosynthesis, by which light energy is used to convert inorganic compounds into organic substances with the release of oxygen, may be the most important biological event sustaining life (Keir et al. 2017). In the light-dependent reactions, the chloroplasts of a plant use the pigment chlorophyll to convert light energy into chemical energy. This energy is used to split water and produce oxygen (Eller et al. 2015). The energy is later used in the light independent reactions, where carbon dioxide (CO2) undergoes carbon fixation with the aid of enzyme rubisco, because it catalyses both carboxylation and oxygenation reactions and most of responses of photosynthesis to light, CO2, and temperature (John Evans 2013).

University Press, Cambridge, United Kingdom. E J H Corner, 2002. The Life of Plants. University of Chicago Press,

War, A., Paulraj, M., Ahmad, T., Buhroo, A., Hussain, B., Ignacimuthu, S. and Sharma, H. (2012). Mechanisms of plant defense against insect herbivores. [online] NCBI. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3493419/ [Accessed 10 Mar.

A., & Kiss, J. Z. (2012). Phototropism and gravitropism in transgenic lines of Arabidopsis altered in the phytochrome pathway. Physiologia Plantarum, 145(3), 461-473. doi:10.1111/j.1399-3054.2012.01607.x

Sterol C-22 desaturase plays an important role as terminal enzyme in the sterol biosynthesis in fungi and plants. Fungal cytochrome P450 monooxygenase, CYP61, is the sterol C-22 desaturase, the penultimate enzyme in the ergosterol biosynthetic pathway 14, 16. Erogosterol synthesis involves lanosterol synthase needed for cyclization of 2,3-oxidosqalene and CYP61 for C-22 desaturation. In case of plants, the first cyclization of 2,3-oxidosqalene is mediated by cycloartenol synthase and CYP710 (a C-22 desaturase) for C-22 desaturation 11. Interestingly, Leishmania and Trypanosomes being non-photosynthetic protozoans not only synthesize lanosterol but also contain CYP710 (L. major, XP_001684965) related genes 17, 18. Presence of CYP710 related gene suggests the possible role of these C-22 desaturases in the synthesis of stigmasterol in these

Many species of plants host microorganisms living inside the plant forming a mutually beneficial endosymbiosis. Bacteria or fungi that reside within plant tissue (roots, stems, and/or leaves) are referred to as endophytes. These endophyte communities may help to improve a plant’s fitness by promoting growth, protecting against disease, or facilitating nutrient acquisition. More specifically, endophytes within the plant community can help plants respond to stress that develops from biotic or abiotic influences like pests, heat, drought, saline, and soil conditions (Russell et al., 2003) Endophytes can help plants become better able to tolerate stress by allocating resources from one place to another (Rodriguez et al., 2009). Therefore,

Plants have evolved sophisticated genetic and epigenetic regulatory systems to respond quickly to unfavorable environmental conditions such as heat, cold, drought, and pathogen infections. In particular, heat greatly affects plant growth and development, immunity and circadian rhythm, and poses a serious threat to the global food supply. According to temperatures exposing, heat can be usually classified as warm ambient temperature

The tobacco plant like many plants contain a cell callus. A cell callus contains somatic undifferentiated cells and can be used to differentiate into specialized tissues of the tobacco plant, or any plant used, by being induced with the addition of different types of hormones, such as cytokinin and auxin. Cytokinin and auxin are mostly used in plant tissue culture simultaneously to provoke the formation of a plantlet or callus. There is a common use of Kinetin in plant tissue culture since when added it will promote cell division to initiate shoot tissues from calluses of the plant. Kinetin is a type of cytokinin hormone. In regards to auxin related hormones, Indole-3-acetic acid (IAA) is also commonly used since it promotes the initiation of roots for the root tissue of the plant. In this

This data shows a strange outcome, in the hypothesis; it says that “If acid is introduced to the seed during germination, then the roots will not grow as long as the seeds that are given water”. This statement proves to be untrue, because the roots grew longer with stronger acid than weaker acid, and in some, cases, grew better with strong acid than it did in water. This may be true because of the acid growth theory. The acid growth theory states that auxins cause the elongation of stem cells by promoting wall loosening. It was determined that this wall loosening is caused by hydrogen ions. This idea and subsequent supporting data gave rise to the acid growth theory, which states that when exposed to auxins, susceptible cells excrete protons into the wall at an enhanced rate, which in turn decreases the pH in the wall. The lowered wall pH then activates the wall loosening process which is essentially doing the same thing as the auxin hormone.

All plants are subjected to a multitude of stresses throughout their life cycle. Depending on the species of plant and the source of the stress, the plant will respond in different ways. When a certain tolerance level is reached, the plant will eventually die. When the plants in question are crop plants, then a problem arises. The two major environmental factors that currently reduce plant productivity are drought and salinity (Serrano, 1999), and these stresses cause similar reactions in plants due to water stress. These environmental concerns affect plants more than is commonly thought. For example, disease and insect loss typically decrease crop yields by less than ten percent, but severe