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A Study On A Gel Electrophoresis Experiment

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1. E O E­O (E­O)^2 (E­O)^2/E
Green 47 53 ­6 36 0.766
Golden 47 41 6 36 0.766
Total 94 94 1.532
Chi­square=1.532.

2. Allele for red: a, allele for green: A.
Because the proportion of red beetles (aa) is equal to 0.23, the frequency of a allele is (0.23)^0.5=0.48.
Since the population is in Hardy­Weinberg equilibrium, the frequency of the red colored allele will not change and will still be 0.48.

3. A. A gel electrophoresis experiment works because DNA has negative charge and is attracted to the positive electrode. The different mass of DNA segments with different lengths separates them as they move across the gel. Shorter DNA segments move further toward the end of the gel while longer DNA segments lag behind. This experiment is set up by preparing for a gel with agar solution of a certain concentration and putting two electrodes at its two ends. The gel in the question would be able to prove the defendant guilty because the pattern of the gene found at the crime scene completely matches the defendant’s, meaning that the gene found at the crime scene is from the defendant. Thus, the defendant is indeed guilty.
B. When we compare the DNA pattern of the father and the son, we should be able to see a lot of similarity because a father passes down his genes to his son and result in two sets of highly similar DNAs.
Lab procedure: prepare for agar solution, make the gel with wells on one side. Put the gel in the water into an electric field, wells on the negative side. Collect

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