A Study On Aging Associated Changes And Development Of Identified Neurons During Aging

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(C1.1) Preliminary results: Electrophysiological measurements in the intact abdominal ganglia and analysis of electrophysiological changes in identified neurons during aging. We recently described a ganglia preparation to carry out electrophysiological and molecular analysis of the same neurons in the abdominal ganglia. Using this methodology [31], we have carried out intracellular recordings from L7, L11 and R15 neurons (Fig 2). We then focused on aging associated changes in R15 and have analyzed membrane potentials and response to neurotransmitter Acetylcholine (Ach). We compared two different age groups, mature (6-7 months) and old (11-12 months) animals and found that aging did not alter basal properties such as membrane potential or latency and slope of burst firing whereas we observed a decrease in Ach response in old R15 neurons. To gain deeper insight into Ach induced changes in burst firing; we analyzed four parameters of action potential (AP) waveform (amplitude and duration of depolarization and repolarization). We observed specific changes in AP waveform [32]. For example, Ach induced changes in duration of depolarization and repolarization in mature R15 was absent on the old R15 (Fig 3) suggesting a decrease in response to Ach during aging [32].
Multiphoton fluorescence imaging of L7MN in intact abdominal ganglia. As an initial step to trace L7MN in the ganglia to study morphological changes during aging, we have microinjected fluorescent dextran (Alexa Fluor

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