A Study On Molecular Reactions

2324 Words Oct 6th, 2016 10 Pages
A majority of biochemical reactions require enzymes. Enzymes are biological catalysts that speed up reactions by lowering associated activation energies.1 More specifically, enzymes bind substrates and hold them in an orientation that increases the likelihood of them coming into contact with other reactants.1 A way of measuring how fast an enzyme works is by conducting an enzyme assay. An enzyme assay is the process by which a spectrophotometer is used to determine how the concentration of a colored substrate or product changes within an enzyme solution over time.1 With that being said, the purpose of this experiment is to perform multiple enzyme assays in order to determine the concentrations of unknown solutions and to gain an understanding of how different enzymes work. For the first part of the experiment, a phosphatase assay will be conducted by combining p-Nitrophenol Phosphate and a phosphatase, an enzyme that hydrolyzes phosphate groups. Since one of the products of the reaction, p-Nitrophenol, absorbs light at 405 nanometers (nm), an increase in absorbance at 405 nm indicates the formation of the product. Thus, by measuring the increasing absorbance at 405 nm, we can use the Beer- Lambert law (A=ɛlc) to quantify how much product is produced at specific times. The second part of the lab involves conducting an alcohol dehydrogenase (ADH) assay using a dehydrogenase, an enzyme that catalyzes the removal of hydrogen atoms from a particular molecule. To do this,…
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