pGLO Pig Slurry
Abstract pGLO is a plasmid that contains several genes, araC, gfp,bla, and an ori of replication. E. coli was artificially induced that became a competent bacteria when it took the pGLO DNA, so it had the ability to have ampicillin resistance and fluoresced when arabinose was present. Two tubes with E. coli were labeled to differentiate which tube the pGLO was added to, then through several steps the bacteria was induced to intake the pGLO DNA. At the end, each tube was inoculated on to three different plates that contained different substances and they were incubated then observed. The results showed only one E. coli culture had growth and fluoresced which was the pGLO+ E. coli that was grown on the plate with LB, amp +arab, there were only two E. coli cultures that did not grow because the pGLO- E. coli did not have the ampicillin resistance to grow in ampicillin conditions, and the rest of the culture plates showed growth. pGLO could be used in food safety experiments done by the food safety department that helped to identify Salmonella and Yersinia enterocolitica in pig slurry, so they were able to calculate how long these bacteria lived in certain conditions before disinfectants were used.
Introduction Transformation is a process when a bacteria cell was able to be incorporated into a sequence of DNA from the environment, this type of bacteria is called a competent