Essay about AP Investigation Lab #13 Enzyme Activity

1929 WordsNov 26, 20138 Pages
Background Information Part 1 In the first part of the enzyme lab, we mixed a substrate and an indicator with an enzyme. There was also a neutral buffer in each of the chemical mixtures. The neutral buffer regulated the pH to around 7. We got a color palette and once we mixed each together, we observed and saw a change in the color of the substance. The darker and more brown the substance got, the more oxygen produced by the reaction. Our results showed that amount of oxygen produced increased about 10% a minute until it sort of equilibrated at 4 minutes and didn’t change to the fifth minute mark. If we were to change anything we did in the experiment, we would make our comparisons to the chart more precise. Overall we thought it…show more content…
2. We inverted twice to mix the SPBN test tube 3. We measured 2.5 mL of turnip peroxidase (the enzyme) and 10 mL of neutral buffer (pH 7) with a syringe and disposed it into test tube ENB. 4. We inverted twice to mix the ENB test tube. 5. We disposed the contents of test tube SPNB into ENB using a disposable transfer pipet and inverted twice to mix the solution 6. In 1 minute intervals, we observed the color changes of the reactions and compared them to the color palette. We did this for 5 minutes. Part 2 1. We measured 2 mL of diluted hydrogen peroxide (the substrate), 1 mL of guaiacol (the product indicator), and 1 mL of neutral buffer (pH 7) with a syringe and disposed it into tubes 1, 2 , 4, 9, 11, and 12. 2. We measured 1 mL of turnip peroxidase (the enzyme) and 3 mL of neutral buffer (pH corresponding to the test tube number i.e. pH 5 in test tube 5) with a syringe and disposed it into tubes 3, 5, 6, 7, 8, and 10 3. We poured tube 1 with the solution in tube 3 to combine them. We repeated this for all of the tubes. Each of the tubes in step 1 was mixed with a tube in step 3, making there be 6 total test tubes with a solution in it. 4. We observed the test tubes and compared the colors produced from the reaction to the color palette in 1 minute intervals for 5 minutes total.

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