Absorption Spectroscopy: Beer-Lambert Law Essay

My results yielded a high Average Molarity .270M . The ideal would be around 1.000M . Deviation was ± 1.20

From this graph and chart we can see that the higher the concentration the higher the absorbance, all the different concentrations were tested at the same wavelength (625nm). Also we can determine our unknown substances concentration by using the absorbance we got for it. The red dot on the graph followed by the line towards the horizontal axis indicates that the concentration of fast green was 34% or 5.1x10-3.

10 microliters of the sample is then added and the assay absorption is measured at 340nm. If absorbance was above 1.5, samples were diluted.

The procedures for experiment A, B, and C all start the same. The first step is to put on goggles and get the data collection device set properly. The labquest needs to be plugged into the colorimeter accurately so that a click is heard when putting it in. The labquest needs to be reading digitally and the colorimeter needs to be set to 635 nm. Then shake the chloroplast solution and take a clean cuvette and fill it with 3 mL of distilled water, 3 drops of the chloroplast solution, and cap it. This is used as a blank to calibrate your labquest. Double check that the labquest is reading absorbance, this assures that the colorimeter is plugged into the labquest accurately. Insert the blank into the colorimeter and hit the calibration button. Take out the blank and empty it. The labquest is now set to experiment with. Make sure that the heat bank is set in front of the lamp and that the lamp is on. The cuvette must be placed on the opposite side of the heat bank in the path of light in the box so that no other light can interfere with the experiment.

For this experiment, the amounts of Red 40 and Blue 1 were quantified in six different Kool-Aid samples through the use of a spectrophotometer. This was completing by performing serial dilutions on both dyes, Red 40 and Blue 1, and then creating calibration curves for each of the six samples. The absorbance and maximum wavelength values were obtained from the spectrophotometer for each individual drink sample. Beer’s Law was used to discover the concentration of

Scientists use an instrument called a spectrometer to quantitatively determine the amount of light absorbed by a solution. The primary inner parts of a typical spectrometer are described below. The spectrometer has a light source that emits white light containing a vast mixture of different wavelengths of electromagnetic radiation. The wavelength of interest is then selected using a monochromator (“mono” meaning one and “chromate” meaning color) and an additional exit slit. The separation of white light into different colors (wavelengths) is known as diffraction. The selected light then reaches the sample and depending on how the light interacts with the chemical compound of interest, some of the light is absorbed and some passes straight through. By comparing the amount of light entering the sample (P0) with the amount of light reaching the detector (P), the spectrometer is able to tell how much light is absorbed by the sample.

An experiment was done to determine the concentration of Allura Red in a commercially available beverage, Gatorade. This was done by using the colorimeter, the absorbance of five Allura Red solutions were measured. The graph obtained demonstrated a linear relationship and the equation obtained yielded a concentration of 1.9910-6M.

The Beers Law calibration experiment used many concentrations of crystal violet solutions. Each of these solutions were test and analyzed in order to determine the absorbance of each concentration The results were than graphed and produced a slope of 1.00E05 with an intercept of -2.21E-02.

Introduction: The goal or purpose of this experiment was to determine the concentration of Allura Red in red commercially available beverage- Gatorade. Colorimeter are used to shine a LED light through the solution and hit a photocell: it will detect an absorbance or a percent transmittance value. These “value” can be charted and examined as a calibration curve. Calibration curve is a method for determining a substance concentration in an unknown sample

To be able to know how to conduct their experiment, the scientists researched how to use certain materials they will be using. One of the main materials they will be using will be a refractometer to measure the TDS of the black coffee and gun powder green tea. It works by putting a few drops of the beverage onto the sample well of the refractometer, where a small light beam shines through. The refractometer then calculates the amount of total dissolved solids and provides the percentage of TDS. (Fresh Cup Magazine,

In this experiment 10 cuvettes were filled with the appropriate dilution, an additional cuvette should be filled with distilled water which should be used to calibrate the colorimeter. Record the absorbance and transmittance for each dilution generated from the Webquest. Additionally, two samples of Gatorade contains Blue Dye #1 (Low Calorie and Glacial Frost) were tested and found that the Low Calorie Gatorade had an absorbance of .135 and the absorbance of the Glacial Frost Gatorade was .153. Using linear regression the concentration of the two samples can be found if the value of transmittance is substituted for the Y value. The concentration of sample 1 is 62.8 µM and the concentration of sample 2 is 69.68 µM respectively reporting in four significant

The main purpose of the food dye experiment was to identify the different food dyes; red, blue, and yellow; and their concentrations that make up the given liquid and use that data to recreate the liquids color profile. The liquid that was to be tested and recreated was Listerine mouthwash. To determine the food dyes present in the mouthwash use a UV-visible spectroscopy which will give the absorbances of each wavelength. With the absorbance find the concentration of each color dye by using Beer’s Law. To find the volume need for each dye use the equation M1V1=M2V2 to recreate the

     After the column the separated compounds enter the detector, which measures a physical or chemical property of each, now relatively pure, compound and creates a proportional electronic signal. By calibrating with a standard mixture of known compounds, the nature of the compound in the

Crystal violet is a common purple indicator used to observe the rate of a reaction. The color intensity of the dye slowly fades and the solution becomes colorless as a reaction progresses. The kinetics of this “fading” reaction can be analyzed by measuring the absorbance or

Remove foam plug from the inoculate flask and pour it into a cuvette. Incubate it at 37°C and the optical density readings at 600nm every 10-15 mins. Pour into the