Essay about Acid/ Base properties of an antimicrobial agent

863 Words Sep 23rd, 2013 4 Pages
Chem 253
Lab 1: Acid/Base Properties of an Antimicrobial Agent
09/03/13

Purpose The purpose was to determine the acid and base properties of the antimicrobial agent sodium benzoate. Theory Sodium has mainly been used in food processing to avoid growth of bacteria and harmful microorganisms. It is commonly used to preserve foods and beverages that have an acidic pH. Rather than benzoic acid, sodium benzoate is used because it is generally soluble in most aqueous solutions but benzoic acid is not.

Reaction Sodium benzoate mixed with water, dissociates into benzoate and sodium ions.

Hydrogen chloride, which is a gas, will exist in ionized form when combined with water to form hydrochloric acid.

Then when
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Moisten the filter paper in the funnel with a little distilled water, and make sure that the moist filter paper fits snugly in the bottom of the Buchner funnel with no folds or bends in the filter paper visible. Use the thick rubber tubing from your drawer to provide vacuum from the “aspirator” to your “filter flask.” Be sure you are connecting the tubing to the “aspirator,” not to the water or gas outlet. Turn on the water to a low volume to create a vacuum in the filter flask but not so much that you flood the sink.
6) Transfer the crystals (quantitatively) from Step 4 to the funnel. Use about 5 mL of distilled water to rinse any solid that remains in the beaker onto the filter paper. Let the solid air-dry with the aspirator running for a few minutes.
7) Place the filter paper with solid in the oven for about 15 minutes to dry. Weigh and then dry again for 5 minutes. Weigh again. If your second weight differs from the first weight by more than 0.5%, dry again for 10 minutes. Continue this process until successive weighing’s are within 0.5%.
For this lab, we must be sure to always wear our safety glasses whenever we are in the laboratory and wear gloves when we are conducting an experiment. Benzoic acid and sodium benzoate used in this experiment can be mild irritants to the skin. We must be careful not to get them on our skin or

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