Solution
#9 Clear, colorless solution. P; Purple
Protein
Solution (+control)
#10 Clear, yellow-orange solution. N
Beer
#11 Clear, colorless solution. N
Distilled
Water(-control)
#12 Clear, colorless solution. P; Purple
Unknown
*Table #2 shows the results of biuret test on the given 12 samples, in which 2 are indicated positive: sample #9, the positive control of this section, and sample #12, the unknown.
Table #3 Iodine Test for Starch and Glycogen in the Given Samples
Samples Observation of Samples
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P; Reddish Brown
1% Glycogen
Solution
#8 Clear, colorless solution. P; Blue-Black
1% Starch
Solution
#9 Clear, colorless solution. N
Protein
Solution
#10 Clear, yellow-orange solution. N
Beer
#11 Clear, colorless solution. N
Distilled
Water(-control)
#12 Clear, colorless solution. N
Unknown
*Table #3shows the results of iodine test on the given 12 samples, in which 2 are indicated positive: sample #7, the glycogen solution, and sample #8, the starch solution. The two can also be considered as positive controls for this section.
Table #4 Benedict 's Test for Oligosaccharides in the Given Samples
#1 Clear, colorless solution. P; Brown
1% Glucose
Solution (+control)
#2 Clear, colorless solution. N
0.3% Glucose
-1-phosphate
#3 Clear, colorless solution. P; Reddish Brown
1% Maltose
Solution
#4 Clear, slightly yellow solution. P; Orange
Honey
Solution
#5 Clear, colorless solution. N
1%
5. What reaction would you expect when performing a negative control in the catalase assay?
To perform this test, a tube of broth rich with glucose is acquired. In this tube is phenol red, a pH indicator. Initially, the tube appeared pink in color, indicating a normal pH level. Next, a sample of unknown #44 is introduced into this medium using the aseptic technique, and this is allowed to sit for several days. If the organism is able to ferment glucose, the pH in the medium would decrease and cause the phenol red to exhibit a yellow color. In addition to the straw color, gas can also be produced and trapped inside the Durham tube placed in the medium. This production of acid and gas is a direct result of the fermentation of glucose, as seen with unknown
The homogenate used in this experiment was liver homogenate. Five different samples and a blank
Objective(s): The reason for this experiment is to see how starch and iodine affect each other and how a plastic bag works similar to a membrane in certain situations.
2. Which of the lab values gives you information about how Mrs. Burroughs’ kidneys are functioning?
This experiment consisted of setting up a control group of starch in various temperature and then placing both fungal amylases and bacterial amylases in a mixture of starch and placing the solution of amylase and starch in various temperatures of water. After a certain amount of time- different amount of time needs to be used in order to have reliable results- iodine is added in a well on spot plates, then two drops of the mixture of amylase-starch is added from each temperature used, by adding iodine into the plates the mixture will show how much starch was hydrolyzed, this is used to calculate the amount of
For this experiment, we have to prepare our phosphorylase which extracted from a potato. We prepared by weighed about 250 grams of peeled potato and cut it into cubes. The extracts then blended with 100mL of 0.1M NaF. After filtered the contents into a clean 250mL centrifuge bottle, we centrifuged it for 3 minutes. Then, separated the supernatant into a centrifuge bottle, which is our phosphorylase preparation. The enzyme assay used in this experiment today is the iodine test. As the iodine reacts with starch, it will form a brown, blue or black precipitate due to the iodine ions forcing into a linear arrangement. The endpoint of the enzyme reaction indicates the presence of starch by using the iodine test to determine. The faster the endpoint is reached, the less active the phosphorylase is.
After carefully taking them out, observe each test tubes color and then fill Table 2 with the results.
Therefore Iodine can be utilized to detect the presence of starch since the reaction of I2+ I- gives us I3 - . What this means is that any substance that becomes blue/black after iodine has been added to it proves that the substance has starch in it. Interestingly enough, when Iodine is added either to white rice or sweet potatoes, these two foods turn to the blue black color that confirms the presence of starch. However, If I add iodine to a banana, it only turns yellow which confirms that the banana does not contain iodine.
The null hypothesis will be that the test tubes with an increase in temperature, pH values, enzyme concentrations, and substrate concentration will have a very small color change or no color change at all. The alternate hypothesis is that the test tubes containing an increase in temperature, pH values, enzyme concentrations, and substrate concentration will all have an intense color change; the more the change, the more intense the color change will be.
"Try new things" has always been an important saying to me. If you never venture outside of your comfort zone, you might never realize how much something could positively impact your life. That being said, I strongly agree with Ralph Waldo Emerson's statement, "Unless you try to do something beyond what you have already mastered, you will never grow." Emerson's statement relates to modern day science. In the book "Acid Test" by Tom Shroder, the author informs the reader on the use of psychedelic drugs, such as LSD and psilocybin, to treat mental illness. With the population's intense adversity to the use of these drugs, testing of this theory has been made incredibly difficult. However, in the tests that have been executed, these drugs
(6.2)Material and Methods in the process or exercise of measuring the starch we were used the following material and how we used them to conduct the experiment. Obtain seven tubes the material to be tested table 6.1 and then add seven to ten drops of iodine to each tube, and then record the color of the tubes contents in table 6.1
2. Following solutions are added to the tubes and the pH of each tube is determined:
Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.
Carbohydrates are the product that made up from carbon, hydrogen and oxygen. Carbohydrates are form by the combination of carbon dioxide and water molecules. The carbohydrates contain two specific functional group in it which is the hydroxyl groups and carbonyl groups.A reducing sugar is a type of sugar with is an aldehyde group.This means that sugar can act as a reducing agent.The procces of reducing sugar is isomerisation,example of reducing sugar islactose,maltose,glucose and fructose.All monosaccharides are capable of reducing other chemicals such as copper (II) sulphate to copper oxide.Beside that disaccharides such as maltose and lactose are reducing sugar,however sucrose is non reducing