Advantages And Disadvantages Of Haematoxylin

It is a chemical reaction where the colors of both solution and iron strip changed. It is

294). This means even after a week the blood has been cleaned up, luminol can still detect it. Also after applying luminol, the area needs to be darkened so that blue glow can be emitted. “The iron from the hemoglobin in the blood causes luminol to glow, so a blue glow is produced when the solution is sprayed where there is blood. Only a tiny amount of iron is required to catalyze the reaction” (Helmenstine, 2012). The use of luminol revealed that there were blood stains on the bed sheets and suspected that the woman was killed in her bedroom and that she did not go to work that day or leave the house.

To perform this test, a small drop of water is placed on a clean microscope slide. A metal loop that has been properly sterilized in the blue flame and allowed time to cool is used to

The first disease Moalem discusses, hemochromatosis, is a hereditary disorder that he himself lives with. It causes excess iron buildup throughout the body, damaging “the joints, the major organs, and overall body chemistry” (p. 13). Hemochromatosis can lead to “liver failure, heart failure, diabetes, arthritis, infertility, psychiatric disorders[, ...] cancer” (p. 13) and, in the end, death. Although it is more common in some communities than others, today the low-penetrance disease only manifests in 1 in 200 people.

1) Apply the stain to your first unknown slide and examine it under the microscope.

Then we carefully submerged the nitrocellulose sheet on the transfer buffer for about 5 minutes, and set up the transfer apparatus in the method shown in the lab outline week 2 step 3-6. Afterwards we took apart the transfer apparatus and detached the nitrocellulose sheet. Then we put the nitrocellulose sheet for 5 minutes into the blocking buffer, and we left nitrocellulose sheet in a container till week 3. The second part of week 2 was staining half our PAGE gel from week 1. Which consisted of pouring deionized water and use the shaker machine for 5 minute intervals, and repeating the the process three times. Then the Fresh Coomassie stain was added to the gel and was rinsed (UFV BIO 202 Lab,

The drops of crystal violets, approximately 15 drops, were flooded until the smear were all covered and then allowing resting for one to two minutes. After two minutes, the slide was titled over the sink and washed off, with the distilled water bottle, by aiming the stream of water above the smear. The specimen appeared blue-violet when observed with the naked eye. The drops of Gram’s iodine were applied on the slide until covered and then allowed to react for one minute or more. After the time elapsed, the slide was rinsed again with distilled water following immediate drops of Gram stain decolorizer added one drop at a time.

Group 1, the gel picture and Andersons experiment are closely related. The binding did occur, in the 1st lane of group 1the dye was free therefore no binding occurred because no Bovine Serum Albumin (BSA) was present in the mixture. While in lanes 2 to 4 binding did occur because there was BSA in the mixture, with the higest binding occurring in lane 4, this is because there was no buffer to interrupt the binding. (saturation).

The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect

Figure #1 This picture show the gram stain of unknown #3. The image is under 1000x magnification using oil immersion technique. Gram (-)

Staining the cells with dye allows us to visualize the structures of the cell more precisely under a microscope.

In criminal shows shown on television, almost common in every show are investigators inspecting crime scenes. Often when we see investigators looking for some form of a bodily fluid at a crime scene, they spray some liquid and then use a black light over the area. If bodily fluids are present, then the area appears to glow. The substance that they are spraying from the bottle is in fact luminol, and the glow that is observed is from a chemiluminescent reaction. The blood illuminates because of iron in the hemoglobin. Iron serves as the catalyst for the chemiluminescent reaction and causes it to glow .

1964). There was a statistically significant effect of time spent in the blocking solution and the fixative and staining quality. In future experiments, the time samples are placed in blocking solution and fixative should be monitored more closely and left for a sufficient amount of time to get better and more accurate results, concerning staining quality. On the contrary, there was a positive correlation between the amount of time the samples were left in the antibody solutions and staining results. The longer the samples were left in the antibody solution, the better the staining results were as attested by the statistical

diH20 acted as a negative control in the experiment, all cells, both in intrafollicular zone and in interfollicular zone stained purple. Cells stained brown in the negative control indicate non-specific staining.

The materials used for the first part of the experiment comprised of the following: a microscope, 4 slides, 4 slide covers, blood samples, lancet, a sheet of paper towel, 3 test tube droppers, Solutions A, Solutions B, and Solution C.