Advantages And Disadvantages Of Solid Phase Extraction

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Solid-Phase Extraction (SPE) is a sample preparation step which is important in determination of specific compounds in foods. SPE offers many opportunities not only for analysis of a large diversity of food samples but also for optimization and advances. 5 Besides, Solid-phase extraction methods for DNA have been developed in recent years in formats that enable high-throughput DNA extractions. Moreover, Solid phase extraction (SPE) is widely used for clean-up in food analysis, and has largely replaced classical gravity column chromatography. 6 Furthermore, the applications of SPE are determination of sympathomimetic amines in blood, urine and tissues, Benzodiazepines screening in urine, determination of cocaine and cocaine metabolites and anabolic steroids in urine and Lysergic acid diethylamide (LSD) in blood, serum or plasma.
Liquid-liquid Extraction Liquid – liquid extraction is also known as solvent extraction. It is a separation process which is based on the different distribution of the components to be separated between two liquid phases. It depends on the mass transfer of the component to be extracted from
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For direct extraction, dip the fibre coating into the aqueous sample and the analytes can partition between the coating and the matrix. For headspace extraction, place the fibre in the headspace above the aqueous matrix during extraction. Extracting the volatile analytes only, but this method is advantageous for high molecular weight interfering samples. When a sample contains both high molecular weight interfering and non-volatile compounds, such as proteins, it is quite difficult to applied direct or headspace SPME. In such cases, restricted-access materials or membrane-protected SPME is used and a better reproducibility and accuracy result will be
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