Advantages Of Homogenization In Proteomics

10 microliters of the sample is then added and the assay absorption is measured at 340nm. If absorbance was above 1.5, samples were diluted.

The unknown sample was then heated in boiling water in step 5. The reason for heating the

Me and my lab partner, obtained a mixture of a un known proportion from the instructor and then flow the guide line in our lab manual to separate the mixture by applying the separation method motioned in our lab manual pages 33-40 . In this experiment, the separation methods were decantation,

Genomic DNA is heterogenrous because it shows 2 fragments on 2% agarose gel which come from parents, mom and dad. Moreover, the tandem repeats(n) is within the standard limit (14-41) of heterogenic DNA. So, the sample is heteregenerous.

The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly

The protein/DNA ratio indicated a greater ratio in the heart homogenate in comparison to the liver and kidney. The relative cell size in the heart homogenate could be inferred to be much greater because of such a high ratio. (Table 1)

tralization solution and centrifuging it for 5 minutes. The Wizard miniprep kit is used to bind the

The homogenate used in this experiment was liver homogenate. Five different samples and a blank

The purpose of this experiment is to familiarize oneself with the general procedures determining a partition coefficient at the microscale level and learn in weighing milligram quantities of materials on an electronic balance, the use of automatic pipets, the use of transfer pipet, and the use of a vortex mixer. Also, to familiarize oneself with extraction

• It's an expensive technique as it requires costly HPLC instrumentation, columns and also use of highest grade of purity solvents, buffers, chemicals etc termed as HPLC grade.

of being able to analyze multiple samples in a short amount of time. The most efficient way of determining concentration is to prepare a set of standard solutions of known

Gel-Filtration Chromatography is a commonly used method used in order purify a protein from a mixture, by means of separations. Different biomolecules differ in size, or their molecular weight. In the gel matrix inside the chromatography column, there are gel beads which are porous to allow certain sized molecules to enter. The molecules that are able to enter the pores of the gel, are held in stationary phase and will elute from the column later on, these are usually smaller, to medium sized molecules. Larger molecules that are not able to fit in the pores will elute out of the column first, they are involved in mobile phase where they just go straight through the column without interacting with the gel beads. Smaller molecules will have a higher elution volume, while the larger molecules will have a lower elution volume. The volume to elute the protein is inversely proportional to the molecules size.

Major factors influcing cost: press and dies depreciation, maintenance costs and material handling. And thus it is very

The samples were collected in sterile containers and kept in ice box until delivery to the laboratory. Once delivered to the laboratory, the samples were processed for isolation.

For generating valuable data with a desired accuracy and to quantify concentration of the constituents present in the samples being analyzed.