Aeromonas Hydrophila Lab Report

The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes.

According to the “Microbiology Laboratory Manual,” lab 27 focuses on the family Enterobacteriaceae, this is one of the largest families of bacteria (Wong et al., n.d.). Also all of the members are gram-negative, anaerobe straight rods and can commonly be located in mammal’s intestines (Wong et al., n.d.). All the members Enterobacter aerogenes throughout this report will be referred to as, E. aerogenes. There were several examples where E. aerogenes was tested or used, the first example is a One-pot synthesis, the next example is where it is used for dark fermentative biohydrogen production, and the last example is where it is analyzed in groundwater in North Central Nigeria.

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

Yes, the candle jar technique would be useful when trying to identify Campylobacter because this technique is good for the growth of microaerophillic bacteria. The flame of the candle will consume the majority of the oxygen in the jar which will generate a higher amount of carbon dioxide.

Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.

Bacteria are small, unicellular prokaryotic microbes. They have many morphologies, which include rod-shaped, spherical, spirals, helices, stars, cubes, and clubs. Classification of bacteria begins with either aerobic (requiring diatomic oxygen for growth) or anaerobic (not requiring O2 for growth). Bacteria can simply be narrowed down to gram positive (organism that stains purple or blue by Gram stain) or gram negative (organism that stains red or pink by Gram stain). Many physical and nutritional factors influence bacterial growth. Physical factors include temperature (psychrophiles, thermophiles, and mesophiles), pH (neutrophiles, acidophiles, and alkalinophiles), O2 concentration (aerobic

Prokaryotes are ubiquitous, successfully adapting to diverse environments as well as developing symbiotic relationships with host organisms (Lengeler, Drews, & Schlegel, 1999). Prokaryotes may have both autotrophic and heterotrophic characteristics. A cyanobacteria is photosynthetic, commonly called blue-green algae, and may produce toxins (Crayton, 1993). Bacteria are most commonly associated in the general

The bases of this experiment was to discover the identify of the unknown from three possible specimens: Klebsiella pneumonia, Escherichia coli, and Enterobacter aerogenes. Utilizaing the T streak technique, the bacteria was isolated into pure colonies for further study. The Gram Stain method was used to identity the morhphology of the bacteria such as the shape and whether the bacteria was Gram positive or Gram negative. Biochemical test were also used to help identify the unknown bacteria. The biochemical test used was the Triple Sugar Iron Agar, Sulfur Indole Motility test, Methyl Red test, Voges-Proskauer test, Citrate test, Urease test, and the Gelatin test. After observing the morphology of the bacteria using the Gram Stain method and utilizing all the possible biochemical test, the bacteria was identified to be Enterobacter aerogenes.

In order for growth to occur microorganisms need to obtain energy. One of those ways is by either the use of oxygen or not using oxygen. Microorganisms that require oxygen are obligate aerobes. It is essential for

Heterozygotes, which have the wild type phenotype, have normal sight which gives them the advantage of finding a mate and have a better success with attracting a mate with their courtship song (Kyriacou et al, 1978). The male heterozygous Drosophila had a better advantage at mating than the homozygotes, which were the ebony, and therefore we predict there will be more wild type by the end of the experiment.

The bacteria that was contained within Unknown tube #12 is believed to be Pseudomonas aeruginosa, Figure 1. The bacteria tested to be Gram Stain negative, producing a pink, red color retained from the staining process. When the species of bacteria was plated on nutrient media, the cells produced an irregular and spreading configuration as shown in Figure 2. This same plating test provided the margins and elevation, lobate and hilly, respectively. The specimen was stabbed in a Fluid Thioglycollate Medium (FTM) tube using an inoculated loop of the bacteria. The results of this experimentation indicate the type of oxygen requirement of the bacteria. The test found the bacteria to be aerobic as colonies of the bacteria began to form along the top of the FTM tube (Manual 2017).

The study of population genetics is simply how the genes or alleles within a population may vary or change. The purpose of population genetics is being able to predict the genes that are expressed in future generations and understanding why that is. An allele is an alternate form of a gene, so blonde hair and black hair are both alleles but hair color is a gene. In this particular experiment the alleles being focused on are the body colors of the Drosophila melanogaster, which is the common fruit fly. The two different body color alleles are the wild type, tan body color, and the ebony type, black body color.

Aeromonas hydroplilia species are ubiquitous bacteria found in a wide range of aquatic environments. They cause infection in vertebrates and invertebrates, including frogs, birds, and various fish species. Although primarily associated with diseases in immunocompromised humans, new evidences infer that Aeromonas hydroplilia species are potential evolving human pathogens regardless of the host’s status. The Environmental Protection Agency (EPA), and U.S. water supplies routinely examine the organisms. Elevated numbers of Aeromonas hydroplilia species, above the EPA code, are continuously recorded in recreational water samples in San Diego

These are gram positive bacteria which are found in acidic condition of pH 4 to 4.5 – acidophilic.