Allura Red Lab

After the serial dilutions of the red and blue dyes were taken, the molarity and absorbance for both dyes were calculated. Using the MiVi = MfVf equation, the concentrations for each value of the red and blue dye were separately calculated. Calculating absorbances calls for setting the correct wavelengths of light for each dye. In this case, the 470 nm wavelength for red dye and the 635 nm wavelength for blue dye was needed to find the maximum absorbances. The absorbance was found by blanking the colorimeter and entering the concentrations. After both values of the absorbances and concentrations were found, the values were then graphed in order to obtain the equation of the relationship between absorbance and concentration.

The purpose of this experiment is to determine the maximum absorbance of fast green, and the chlorophylls, also in the case of fast green create a concentration curve to determine an unknown substance. Each test will use the spectrophotometer.

3ml of sample was taken first flask at 4 minutes and added to the appropriate tube of sodium hydroxide, from the second flask at 4.5 minute and so on, each flask was sampled at 30 second intervals. The sampling was then repeated starting at 8,12,16 minutes. The final sample from the last flask was taken at 18.5 minutes. Once the sampling was completed, measurements of absorbance were obtained for solution in each tube at 405 nm.

For this experiment, the amounts of Red 40 and Blue 1 were quantified in six different Kool-Aid samples through the use of a spectrophotometer. This was completing by performing serial dilutions on both dyes, Red 40 and Blue 1, and then creating calibration curves for each of the six samples. The absorbance and maximum wavelength values were obtained from the spectrophotometer for each individual drink sample. Beer’s Law was used to discover the concentration of

The Beers Law calibration experiment used many concentrations of crystal violet solutions. Each of these solutions were test and analyzed in order to determine the absorbance of each concentration The results were than graphed and produced a slope of 1.00E05 with an intercept of -2.21E-02.

Introduction: The goal or purpose of this experiment was to determine the concentration of Allura Red in red commercially available beverage- Gatorade. Colorimeter are used to shine a LED light through the solution and hit a photocell: it will detect an absorbance or a percent transmittance value. These “value” can be charted and examined as a calibration curve. Calibration curve is a method for determining a substance concentration in an unknown sample

The concentration of sugars in two well-known sports drinks, Powerade and Gatorade, were determined by monitoring an enzyme-catalysed reaction sequence involving the appearance of NADPH. Sucrose and glucose concentrations were calculated from the concentration of NADPH formed by the reaction of glucose-6-phosphate and NADP+. Spectrophotometric absorbance readings were taken at 340nm, this is because NADPH absorbs strongly at this wavelength, whilst NADP+ does not (1015MSC, 2010). The concentration of glucose and sucrose in

Figure 2: Dye percents versus absorbance in a control, 10%, 20%, and 30% azide solutions.

For this simulation instead of adding predators they were removed which lead to a significant increase in brightness throughout the generations with an average of 16.50±1.69. This further supports the theory that the presents of predators are a dominant factor when determining male brightness.

Once you cross the staffie you are adding other genetic and breed behaviours into the offspring. This can have an effect of watering down or removing the strong anti-aggressive human bond which can result in dogs that are aggressive to adults and children. Many people avoid these dogs on a daily basis as they believe they are a danger to the society and some even shout out rude comments to owners about their dogs. Also some suggest that the blue staffie may not have the same good temperament like the other staffie’s and they are believed to be also known as the Irish Staffies. Some believe that the blue comes from the Pitbull terrier which was genetically bred in many years ago. Staffordshire’s can be aggressive to other animals and that also

Dogs are so much fun but yet not alldogs are happy with the lifestyle they have. Having said that; in their every day life, just like humans, they also need daily exercise. Walking around the neighborhood might work for small dogs, but it is not as suitable for larger dogs. Larger dogs tend to have more energy to burn out than smaller dogs. If they don't have enough space to run around at home, then just walking a couple blocks is not going to make that much of a difference if tied to a leash still restricted to burn out their energy.

Dogue de Bordeaux (French Mastiff); originally bred as fighting dog but also used in war, hunting and guarding. There are several theories over the origin and the date the Dogue de Bordeaux came into existence; some believe the breed to descend from the Greek Molossus (now extinct) or from the Ancient Roman Mastiff. Others believe they originated in the 14th century in France when English Kings ruled Aquitaine and are the result of English Mastiffs crossbreeding with large native breeds of Aquitaine guarding dogs. It is also thought by some that the genes of the Spanish Mastiff were also crossed in later on to produce a more ferocious look for guarding purposes. The ‘Federation Cynologique Internationale’ (FCI) states that the Dogue de Bordeaux

The new products division of Palmer Jackson, Inc., a Cincinnati-based food and beverages manufacturer, successfully perfect a technique for dissolving common antioxidants such as Vitamin E and Selenium into high-glucose beverages. As a result a new line of sports beverages with added benefit of antioxidants occur. Palmer Jackson hired AccuityBrand, a brand consultancy specializing in developing product names and logos, to name and develop creative

To vary the concentrations of each reactant along with the sulphuric acid in order to observe and measure its effect on the overall rate of reaction in absorbance using colourimetry.

concentration, record the absorbance readings at a fixed wavelength, and plot the absorbance vs. concentration data. The wavelength of 520 nm was selected for experiment Part