Ammonia Chromatography Lab Report

When asked to drop one or two drops of a testing solution a number of drops of each testing solution was not constant. This could have made some solutions have a bigger impact on the substances they were mixed with, more than others. Dropping the same amount of drops for each solution would determine the end result of each reaction and which ions are inside the solutions more accurately.

Washing of the copper is necessary in this experiment to separate the iron from the copper and make sure the iron is not counted in the mass of the copper.

One of the most important skills to have in the chemistry lab is the understanding of how chemicals will react. Knowing for example, how a chemical will react with a metal, is an excellent way of determining the amount of a particular metal in a deposit. This knowledge was used in this lab to determine the amount of copper in an unknown sample mixture. It is also known that the determination of the percent concentration of a certain solution, will directly effect the percent transmission and absorption of a solution, dependent upon its dilution. By first testing known concentrations of a solution, and plotting this information graphically, a line is formed

The purpose of this experiment is to practice common organic laboratory techniques inside the lab to get one oriented to the basic methods of procedure that can be used for later experiments. This experiment involves the separation of benzoic acid from a more crude form, consisting of benzoic acid, methyl orange, a common acid/base indicator, and cellulose, a natural polymer of glucose (Huston, and Liu 17-24). The technique that is used to perform this separation is called extraction. Extraction is a systematic process of separating mixtures of compounds, taking advantage of the affinity differences of compounds to separate them (Padias 128-37). This technique recognizes the principle that “like dissolves in like,” that is,

If you put too much of a sample on your chromatography paper, you could possibly have that color bleed into the color next to it, which would mess up your results. If you put too little of a sample on the paper, your color

Pour 50 mL of distilled water into a 100 mL beaker, and then add the unknown substance into it. Mix thoroughly to create the aqueous solution. Now fill a new cuvette with this new solution and place into the SpectroVis Plus device; after wiping the outside with a Kimwipe as usual. Be sure to take note of the absorbance when wavelength is at its maximum. Afterwards, Be sure to take all solutions containing Iron(III) and pour them into the container specified for hazardous wastes. ​

Answer: Once the chromatogram has been completed and is ready to be measured and calculated, on the plate that was used to perform the chromatogram you should see where the red and blue have completely separated. The red food coloring dye should be lower on the plate than the blue food coloring dye.

b) Iron and Barium were present in unknown 3. Assigned unknown reacted with all 4 reactants and formed precipitate with 3 of them (Sodium carbonate, sodium hydroxide and Sulfuric acid). During the experiment it reacted very similarly to Iron (III) nitrate and Barium nitrate. For example, with it was tested against Ammonium Chloride, the color of the solution changed to a light green, very identically to Iron (III) nitrate and Ammonium Chloride. Besides, unknown 3 formed an orange brownish precipitate when it was tested with sodium carbonate. Iron (III) nitrate acted similarly. Moreover, unknown 3 reacted similar to Barium nitrate when it was tested against ammonium chloride and sulfuric acid. It did not form any precipitate with ammonium chloride but formed a very light white precipitate, which is identical to barium nitrate’s reaction against sulfuric acid. Therefore, the two present metal in unknown 3 are Iron and barium.

On a thin chromatography plate, five spots were placed ( as shown in table 2) and the plate was developed using chloroform/methanol. This was later visualized with dragendorff’s reagent under the UV light. All separated components were observed, identified and recorded.

Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance, followed by chlorophyll a. Chlorophyll b is the most polar; therefore, it travels the shortest distance. The separated pigments on the chromatography paper can be eluted in acetone and absorbance spectrum is

The main objective of this experiment is to carry out qualitative analysis to identify metal cations in unknown solution 1.

After wearing the gloves we obtained a chromatography vial from professor and label it with my and my peer initials. We dried up the chromatography vial in fume hood and added 1 ml of chromatography solvent to the vial. Then we took a chromatography strip and measure it 1.5 cm with ruler from one end of the strip and drew a line with pencil we cut two small pieces below the pencil line to form a pointed end. We applied spinach on the strip using quarter to rub the spinach leaf on the line that we drew on the strip and put it into the chromatography vial and placed that in fume hood. We observed as the solvent was moving up the chromatography strip by capillary action. When the solvent was reached approximately 1 cm from the top of the strip then we removed the cap from the vial. We took out the strip from the vial using forceps and marked up the location of the solvent front because it evaporates quickly. We measure out the distance as well as the pigment in order to find out the rf value. Moreover we compared rf values to the one in reference list in order to identify the

Quantitative analysis is the determination of an amount present in a variety of settings. On the other hand, qualitative analysis is the determination of non-numerical information about a reaction, such as observing that a reaction results in a color change. There are chemical changes that separate cations and physical separations, such as decanting, where liquid is poured from a solid-liquid mixture, leaving the solid behind. Centrifuging is another separation technique, where a solid is separated from a liquid by whirling the mixture at high speed. A mixture of metal ions in a solution can be separated by a reaction of precipitation and in this experiment, the metal cations of aluminum, nickel, and iron ions will be separated from a solution

The next step was to place the strip of chromatography paper on a paper towel. Then dip a capillary tube into the plant pigment extract (spinach pigment extract) provided by the teacher. The tube will fill on its own. We applied the extract to the pencil line on the paper, blew the strip dry, and repeated it three to four times until the line on the paper is a dark

Gel-Filtration Chromatography is a commonly used method used in order purify a protein from a mixture, by means of separations. Different biomolecules differ in size, or their molecular weight. In the gel matrix inside the chromatography column, there are gel beads which are porous to allow certain sized molecules to enter. The molecules that are able to enter the pores of the gel, are held in stationary phase and will elute from the column later on, these are usually smaller, to medium sized molecules. Larger molecules that are not able to fit in the pores will elute out of the column first, they are involved in mobile phase where they just go straight through the column without interacting with the gel beads. Smaller molecules will have a higher elution volume, while the larger molecules will have a lower elution volume. The volume to elute the protein is inversely proportional to the molecules size.