An Analysis Of Variation At Transcription Factor Binding Sites

In this experiment we tested to see what the offspring of an unknown cross of an F1 generation would produce. After observing the F2 generation and recording the data we found some of the Drosophila showed mutations, two in particular. The mutations were the apterus wings, and sepia eyes. After collecting our data through observation, a Chi-test was conducted resulting in a Chi-value of 5.1 and a p-value of .2. Since the p-value was greater than 0.05, there was no significant change in the data. This proved that the Drosophila flies still followed the Mendelian genetics of a 9:3:3:1 ratio.

The goal of this study was to induce a deletion in the DMAP1 gene on chromosome two in Drosophila melanogaster through P-element mobilization. The DMAP1 gene may be an essential gene, however not much is known about it. We attempted to uncover the function of DMAP1 by creating a series of genetic crosses and selecting for brown-eyed non-stubble male flies that may have the deletion. To test whether these flies had the deletion, we produced PCR products and ran them on an agarose gel, which resulted as inconclusive. We created a balanced stock of flies homozygous for the deletion to see if the

While studying eugenics, genetics plays a detrimental role in determining heredity. Genetics is the study and behavior of genes ("Genetics"). These genes are encoded pieces of information that comes from both parents, these genes are found on any of the 23 pairs chromosomes; these chromosomes that contain genes form the human genome. 22 chromosomes all contain the same genes although slight variations of alleles, alleles are alternate forms of genes, and the 23rd chromosome contains the information for the zygote's gender.

Thomas U, Jonsson F, Speicher SA, Knust E. Phenotypic and Molecular Characterization of Ser(d), a Dominant Allele of the Drosophila Gene Serrate. Genetics.

Genetics can help answer questions about our traits and why we look different and advance in different ways from each other in the world. Chapter 1 explains the basics about how genes work, and the portrayal of DNA and RNA. Chapter 2 describes RNA more in detail and it consist of the explanation of the human genome. Specifically, Chapter 3 clarifies how evolution works and how it relates to genetic and medical research. Furthermore, Chapter 4 and 5 explains the knowledge researchers have about genes role in health and diseases, and how society is affected with the advances in medicine and science given approximate credit to these researchers.

Genes can either be sex-linked or autosomal. If a gene appears mostly in one sex chances are the gene is sex-linked and if it appears frequently in both sexes it is most likely autosomal. Using Drosophila melanogaster, also known as the fruit fly, we will determine whether the gene is sex-linked or autosomal. Drosophila melanogasters have a relatively short life span and are an excellent organism for genetic studies because it has simple food requirements, occupies little space, is hardy, completes its life cycle in about 12 days at room temperature, produces large numbers of offspring, can be immobilized readily for examination and

Introduction For centuries, researchers have used Drosophila melanogaster, the common fruit fly, to study genetics. The benefits of using the fruit fly includes: its relatively short generation time, its large amount of available offspring for data, it is easy to store and handle in the laboratory and it is easily and cheaply obtained. Cross-breeding of four types of fruit flies were used in this experiment including: wild type males with normal wings vs. vestigial wing females, wild type males with red eyes vs. white eyed females, wild type male with red eyes vs. sepia eyed females, and wild type males vs. wild type females. In basic mendelian genetics, the terms dominant, recessive and sex-linked are used to describe the different types

Dobzhansky, T. (1930). The manifold effects of the genes stubble and stubbloid in Drosophila melanogaster. Z. indukt. Abstamm.- u. VererbLehre 54: 427-457.

Finally it was found that a total of 62.1 % to about a 74.7% of the human genome was covered by either proceed or by the help of primary transcript.

Studies on the genetics of other animals, such as fruit flies, can reveal more about the genes of other animals, including humans. After crossing the flies, a hypothesis can be made based on the data obtained on whether the traits are autosomal, sex-linked, recessive, or dominant. Methods Prior to this experiment, the vials used to hold the Drosophila melanogaster were made.

The Unknown Drosophila Cross Abstract: Genetics is the study of genes, heredity, and variation in living organisms.. Heredity is the passing of traits to offspring from its parents (Bechtel). This is the process by which an offspring cell or organism acquires or becomes predisposed to the characteristics of its parent cell or organism. These characteristics are in a physical sense are known as phenotypes and genotypes in the genetic sense. In the lab, we studied Mendelian Genetics through a common insect of a fruit fly, formally known as Drosophila Melangaster.

The Drosophila melanogaster is one of genetics most studied organisms. This is due to the Drosophila melanogaster being an excellent model organism. The Drosophila melanogaster has a short lifespan and is genetically similar to humans (Adams 2000). This experiment had three major goals. The first goal of this experiment was to determine which eye colors, body colors and wing type are dominant or recessive. The second goal was to determine if the gene for eye colors, body colors and wing type are on an autosomal or a sex chromosome. The third goal was to determine if eye colors, body colors and wing type are physically linked or independently assorting (Morris and Cahoon). First

1993), the latter being the best studied (Varjosalo and Taipale, 2008). Only one gene is found in the fly, with Dhh being the closest related to Drosophila hedgehog (Varjosalo and Taipale,

The four repeat families associated with distinct transcription factors were scanned for the common sequence binding motif and the results showed that specific regions on the repeat consensus had the TF binding motif. Furthermore, the specificity of the binding motif was validated when the TF binding areas of the repeat consensus were mapped; in 17 bound instances of RLTR11B (a sub-family of ERVK), the TF POU5F1-SOX2 was present, and therefore its motif.

Another key study, performed by Darlington et al, analyzed the function of dCLOCK, the CLOCK protein in Drosophila. This study confirmed the identity of dclock, investigated the function of dCLOCK in regulation of the circadian genes per and tim, and demonstrated the function of PER and TIM in an inhibitory feedback loop (Darlington et al., 1998). First, a screen for a homolog of the mouse clock gene in Drosophila was conducted using the cDNA library of adult Drosophila heads and a probe of the mClock gene. A high yield of overlap in the clones confirmed the presence of a homolog of the mClock gene in Drosophila (Darlington et al., 1998). A Southern blot of Drosophila genomic DNA with a dclock probe produced only one band, indicating that dclock is in fact a single-copy gene (Darlington et al., 1998). Dclock was then used in a Northern blot to probe RNA from the entire body of Drosophila. Results showed that the probe hybridized to RNA in the head, the body, and the appendages of the fly (Darlington et al., 1998). This showed that the expression of dclock occurs at a variety of locations (Darlington et al., 1998). The expression of dclock was then analyzed in terms of the light-dark cycle. Expression patterns during the 24 hour time period confirmed that dclock oscillates with the circadian rhythm (Darlington et al., 1998). After the initial tests to establish dclock as a circadian gene were completed, the function of the protein dCLOCK in the regulation of transcription of