An Observation of Development of Danio Rerio

1. Many experiments were conducted during the 1950s and 1960s with chick embryos and they showed that two patches of tissue essentially controlled the development of the pattern of bones inside limbs.  Describe at

When society thinks about starfish, perch, chordate, and fetal pig they become extremely curious about how their bodies operate because of how they are made up. I will give a brief synopsis of all animals before going into major detail about them. According to the online website named dictionary.com, Starfish are any echinoderm of the class Asteroidea, having the body radially arranged, usually in the form of a star, with five or more rays or arms radiating from a central disk; asteroid (dictionary.com). It is known that a chordate is an animal belonging to the phylum Chordata, composed of true vertebrates and animals having a notochord (dictionary.com). According to research, a fetal pig is an animal in the phylum Chordata and class Mammalia (dictionary.com). A perch is known to be a certain kind of fish with very spiny fins (dictionary). Starfish, perch, chordate, and fetal pig are some very interesting animals that possess some exclusive qualities both similar and different.

animal in the early stages of growth when it’s basic structure and organ development is forming.

Brine shrimp are used as live food for aquatic life in aquariums. Brine shrimp are usually easy to hatch out, but water types may make a difference in their viability. This study is being conducted in order to see if there is any significant difference in the types of water used in hatching out brine shrimp eggs. In which type of water do brine shrimp eggs hatch best? By studying the different types of water that could be used and knowing which one provides a better hatch rate, a benefit will be seen by using the best type of water to hatch out future brine shrimp. I believe this project will set out to prove that brine shrimp eggs will hatch best in ocean water.

Through repeated measures ANOVA test, it was observed that Gosner stages from week one and week two were significant over time, and were also significant between treatments. However there was no significant interaction between treatment and time as the p-value was greater than 0.05 (p=0.1589). These findings support the hypothesis in that during week two the tadpoles were at a higher stage of development after they were subjected to the excess thyroxine, suggesting quicker metamorphosis. This is reasonable as

Using a salamander, proved that the cell nucleus from an early embryonic cell can effectively substitute for the nucleus in a fertilized egg.

Results This lab will record the growth process of embryo rescue in a hormone-free media. The results on the percentages of attempts that resulted in the isolation of an intact embryo was 33%. This is due to the fact that this was the first time that I performed an embryo rescue, and made a few mistakes along the way.

The potential to apply forward genetic approaches for comprehensive genetic dissection of vertebrate development was the initial attraction for researchers to utilize the zebrafish as a model system. Following is an overview of the major forward genetic approaches employing the zebrafish, along with examples that have provided fundamental new insights into vertebrate

At day 3 of the embryo development, a small hole is made on the outside shell of the embryo (the zona pellucida) with a delicate laser beam. On day 5/6, an embryo biopsy is performed. By this time, the embryo should have reached the blastocyst stage which consists of an inner cell mass (forms the foetus) and trophectoderm cells (forms the placenta). The trophectoderm cells should have herniated through the hole made in the outer shell of the embryo on day 3 in which these cells (approximately five cells) are collected for analysis.

The purpose of this experiment was to observe the life cycle process of Drosophila at room temperature (19-22 ˚C) and a cultured temperature at 27 ˚C. These temperatures were used as measurements to determine how rapidly reproduction occurred after one week.

Currently, there are growing research studies within zebrafish pigmentation, neural crest development and cell migration (Rawls 2001). The neural crest (NC) is a multipotent cell that distribute different cell types such as Schwann cells, sensory, glia and pigment cells or chromatophores (Rawls 2011). The zebrafish have three different types of chromatophores: xanthophores (yellow), melanophores (black), and iridophores (silver) (Nord 2016). The zebrafish pigment pattern is divided into two stages: larval and adult pattern. The larval pattern is derived from the neural crest while the adult pigment pattern rises from specific progenitor cells after metamorphosis (Nord 2016). The progenitors of these pigment cells are known to remain multipotent

At the first stage of evaluation, the embryologist observes the general morphology of the oocyte and the number of pronuclei which should be equal to 2. The quality of the embryo is determined by the rhythm creating corrugations and by the morphological characteristics. Sometimes it is possible for an embryo to seem weak the first day after fertilization but later on appears to develop normally.

Zebrafish are able to withstand much higher levels of chemical mutagens that can be tolerated by rodents so it is possible and easy to induce a much higher density of mutations in their genome.

The critical period can be defined as a span of time where a development must occur and if it doesn’t, the organism runs the risk of not being able to develop that aspect of their being at all. One example of this is attachment of baby ducks to the first moving they see after birth. During this critical period, babies must attach to their mothers and if they don’t, they can never reattach to her. Although most of the research surround the critical period was performed on

segregation of cells into groups of special function (tissues). Once the egg is laid, some embryonic development has happened and then usually stops until proper cell conditions are met for the incubation to resume. At the beginning of the stages, the cells are alike, but as the embryo develops, cells are specialized. After the start of incubation, a thick pointed layer of cells become visible caudal or tail end of the embryo. From the primal start, both the head and backbone of the embryo develop. The digestive tract forms and the blood islands appear. Later on, the blood islands would develop into a vascular and blood system. The second day of incubation, the blood islands link and begin to form a vascular system, while the heart is formed elsewhere. By the 44th hour of incubation, both the vascular system and heart join, and the heart starts beating. By the end of the third day of incubation, the beak begins forming. And limb buds for the wings and legs are seen. The chicks body turns 90° and turns it’s left side on the yolk. Both the head and tail get closer so the embryo forms a “C” shape. The mouth, tongue, and nasal pits develop in the digestive and respiratory systems. Even though it hasn’t been enclosed within the body, the heart continues to enlarge. And by the end of the fourth day of incubation, the embryo has all of the needed organs to bolster life after hatching.