An Understanding Of Bacterial Transformation By Plasmid Dna

1874 Words Oct 9th, 2014 8 Pages
Bacterial Transformation Lab Report
Elizabeth Amanquah
Genetics 311
Monday 2:00-4:50
Jan Trybula

Abstract
This study was undertaken to develop an understanding of bacterial transformation by plasmid DNA. This study enables one to observe various traits displayed by transformed bacterial cells. Four experiments were conducted that included (1) Bacterial Transformation, (2) Genomic/Plasmid extraction, (3) DNA Electrophoresis, and(4) Plasmid /Oxidation EMSA. In this study, a transformation of the strain Escherichia coli also known as E. coli will be examined with a special plasmid called pGLO. This study was done to prepare the plasmid into a bacterium and use it to make large quantities by amplifying it. By allowing RNA polymerase to bind to the promoter (operon/ pBAD) and transcribe genes, repressor proteins that are usually turned of can be removed. The survival of the bacteria is based on the natural occurrence of plasmid transformation and does not need to be manually handled. But by weakening the cell membranes of the bacteria through the transduction, transformation and conjugation of the bacteria he natural process can be hastened.

Introduction Transformation has been a very useful technique in areas of bacterial research. It is the process by which the genetic material carried by an individual cell is altered by incorporating DNA in its genome (Hattermann et al. 1990). Transformation in bacterial cells, occur when cells are incorporated into…
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