Analysis Lab Report Sample

6) You want to determine what the optimal pH is for E.coli to grow. How would you set up the experiment? Give a detailed

We found that Tube 1 had an absorbance of 0.085, Tube 2 had 0.034, Tube 3 had 0.027, Tube 4 had 0.032, Tube 5 had 0.025, Tube 6 had 0.028, Tube 7 had 0.022, Tube 8 had 0.024, Tube 9 had 0.030, and Tube 10 had an absorbance of 0.022. We then graphed absorbance vs fraction number so that we could demonstrate the absorbance of each tube and found that Tube 1 had our highest value of absorbance. Basically, the first few tubes have the most protein and then the rest drop off as is shown in the graph.

In this experiment, we were given three main goals to accomplish. We were to identify the compound through a series of organized experiments and analyze as many physical chemical properties as possible.

After viewing the end result of the Gram Stain, I saw that the Gram positive bacterial cells were stained violet, while the Gram negative cells were stained red. In the mixed culture under the microscopes, I was able to notice that the Gram positive cells, Enterococcus durans, were in clusters of short chains. The Gram negative, Proteus mirabilis, were in rod-shaped forms that were spread across the slide. These cells did not seem to cluster.

In the 10-3 pasteurized sample, the plate exhibited 71,000 cells/mL. The results of the additional dilution samples contained too few colony forming units to count. However, in the 10-7 dilution, although the plate demonstrated 12 colonies, there should have been no colony forming units on this plate. The reasons for this could have been that this sample was contaminated from “double-dipping” the sample before dispensing it onto the plate or when using the pipette, it mistakenly was inserted in a higher concentration sample and then immediately to a lower concentration sample before it was dispensed onto the plate.

The petroleum lab and environmental reflect the major focus of the lab well. The abstract concisely communicates the purpose of the lab, the approach, the results, and the significances of the findings. The electrical lab doesn’t have an abstract but sometimes instructors do not require an abstract section. The environmental lab does a better job at writing the abstract because it’s shorter and more direct to the point, therefore easier to understand.

A cell suspension containing 0.5 -1.0 x 10^6 cells/ml in media 10% FBS (Fetal Bovine Serum) was placed on a 24- well plate so a monolayer can be created at the bottom of the wells. The first step will consist of the lab student aspirating the media from the wells making sure to disrupt the monolayer of cells at the bottom of the wells. Next the wells will be washed with PBS to remove dead cells and debris. After the wells are washed 1ml of media (DMEM) in a 5% concentration will be added to keep the cells from dehydrating. 5% Media is used because the goal here is to allow migration and minimize proliferation. Next the lab student will create a horizontal “scratch” along the bottom of the well using a P200 pipette. The lab student should use enough force to crate the scratch but not too much because too much force will create uneven scratches. Finally the treatments will be added to the wells. The plate will then be placed under the light microscope and pictures of the scratches will be taken which will be labeled as (T0) Time point zero. Other pictures will be taken 24 hrs. later at (T24) time point 24. The photos will then be analyzed and compared the lab group to determine if the is any cell

The materials that will be used for this experiment are. Overnight to 24-Hour culture of E.coli in TSB. T4 bacteriophage. We will have 8 screw cap tubes that contain 4.5ml of sterile agar that are melted and cooled in 55C water bath. 8 TSA plates were used. 1 tube containing 3ml of TSB was used. We had 2 screw cap tubes, they each contained 9.9ml of TSB. We also had 1ml pipettes with pipette pumps. We also had 1 15ml sterile centrifuge tube. A Bunsen burner. We also had a incubator that was set at 37C. Some micropipettes were used as well as a table top centrifuge.

How could the student whose report you received have improved his or her lab report?

In this experiment we wanted to observe the effects that different types of solutions have on living cells. The three types of solutions that we were looking for was: isotonic solution………

The results indicate that the MIC of Colistin that is necessary to kill E. coli is actually 0.78 μg/mL. This MIC was concluded because there was no growth present 0.78 μg/mL while the 0.39 μg/mL plate and 1.56 μg/mL plates both showed growth of colonies respectively. The hypothesized MIC was 2.0 μg/mL, but our results point to a smaller MIC of 0.78 μg/mL. None of the other plates exhibited colony growth, but our results showed there was small specs of abnormally growing cells of some sort on plates the 50 μg/mL and 25 μg/mL, and also the 0.78 μg/mL which is this experiment’s MIC, exhibited these abnormal cells. The abnormal growth might be associated with possible contamination of the specimens on those individual agar plates that exhibited this growth since there was no contamination present in the control agar plates.

For our cell counting lab, we used three tubes and label them tube #1, tube #2 and tube #3. Once done we had to take 1ml of cell solution to add to tube #1, in tube #2 we added 0.9 ml of buffer and 0.5ml of the buffer to tube #3. I then took 0.1 ml of cell solution from tube #1 and added to tube #2; to mix we inverted our tube four to five times. 0.5ml of cell solution was taken from tube #2 and added to tube #3 once added we inverted the tube to make sure it mixed well. After mixing the tube I took 10ul of cell solution from the tubes and added it to the hemacytometer, to count our cells in the four 16 squares boxes under our microscope, I repeated the last step for all three tubes. Once our cells were counted, the total number of cells was recorded. Tube#1 cell count was very high it showed 603 cells that were counted under the microscope using the hemacytometer. Tube #2 cell count was low but it showed 299 cells. Tube #3 showed hardly any cells under the microscope 22 cells was counted. After getting the total

Having a chance to participate in chemistry lab is a good thing for people. Based on the research, all the science majors always need to write a lab report after performing a certain experiment. Chemistry is a subject in which we all need to turn in the lab report the to teacher. Based on the lab report, the professor can see how his student understands and applies the lab to life. When writing lab reports, there are certain formats and contents of the lab that must be followed in order to convey the information which is performed. In some cases, lab reports may not be persuasive, so the lab will have to have strong evidence. There are two reasons why writing up the lab reports can be difficult are writing the result and getting a great partner. We have to perform a certain experiment and approach the topic. So the lab report consists of four parts: introduction, procedure, results, and conclusion.

On Plate I (LB) and Plate II (LB + Ampicilin), E.coli growth was expected to occur. Since the environment was not selective in Plate I, growth was expected to be found as lawn. This prediction was confirmed by the experiment. On Plate II, it was expected that either lawn or colonies would be found. The observed growth occured as lawn. On Plate III (YPD) growth was predicted, given the presence of all the nutrients that the auxotrophic yeasts cannot synthetize. Due to the usual non-selectivity of the medium, lawn was the expected type of growth. However, growth actually manifested as red colonies, an occurrence which will be explained in the Discussion section. On Plate IV (YNB, -AA) and Plate V (YNB, -AA, +5% AS) no growth was expected to happen given the absence of amino acids, which the auxotrophic yeast could not synthetize. The results were as predicted. On Plate VI (YNB, +AA-Arg, -AS ), it was expected to obtain colonies, due to the selectivity of the environment. This indeed was observed, in the form of white colonies.

Some lab techniques learned in this lab are decanting, using a pistol grip when transferring beakers to one place to another and during decanting, and using a piece of partially submerged aluminum wire in the copper sample, shaking the wire around, and dislodging any particle of copper precipitate forming on the aluminum wire. Decanting is a lab method used to separate mixtures of solutions and solids by allowing the solid to rest at the bottom of the solution and slowly pouring the liquid out of the container with the help of a glass rod. Solutions are decanted at an angle of approximately 45 degrees so that it allows the solid particle to slowly slide out of the beaker while the liquid can pour out. If a solution was decanted at a 90 degree