Analysis Of Adipose Derived Induced Pluripotent Stem Cells

4532 Words19 Pages
“Research Proposal”
Medical Biotechnology, Health Sciences
Honours Research Proposal 30/06/2015

John Drummond
2040768

1) Grant titles
Scientific title
Analysis of adipose derived induced pluripotent stem cells (iPSC’s) microRNA (miRNA) expression profile over multiple passages and pancreatic β-cell miRNA expression profile post differentiation from iPSC’s.
Simplified title
Improvement of stem cell differentiation to pancreatic β-cell for transplantation into diabetics.
2) Project summary

Ethical concerns regarding the use of embryo-derived embryonic stem cells (ES), and the immune response elicited by differentiated ES cells, are being circumvented by using induced pluripotential stem cells (iPSCs) derived from patients’ somatic cells. These can be differentiated into other cell types including pancreatic -cells for diabetics. Unfortunately aspects of iPSC DNA & regulation of differentiation impair the production of cells for transplant. Understanding the role of micro RNAs in iPSC differentiation could improve the therapeutic application of iPSCs.

3) Keywords

Induced pluripotent stem cells (iPSC’s)
Embryonic stem cells (ES)
Micro RNA (miRNA)
Epigenetics
Pancreatic β-cells

4) Clearance requirements: List any ethical implications & approvals required. Describe each that applies to your proposal (ie. humans, animals, hazardous chemicals, biohazards, OGTR) in ~2 lines.

Human research ethics committee (HREC) application and approval required to collect
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