Analysis of the Absorption of Green Light Versus Red Light Absorption in Spinach Leaves

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Analysis of the Absorption of Green Light Versus Red Light Absorption in Spinach Leaves

The goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. This was done by separating the photosynthetic pigments (chlorophyll a, chlorophyll b, carotene and xanthophylls) from one another using paper chromatography. The separated pigments were then analyzed for their absorption spectrum using a spectrographometer. When the data was graphed it clearly showed the higher rate of red light absorption over green light. These results along with previous research indicate the importance of red light in photosynthesis and the minor role green light plays.

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These wavelengths correspond to the wavelengths of visible light. Overall blue and red light works best, while green in least effective in the photosynthesis process (Nishio, 2000). The wavelengths that a pigment absorbs, absorption spectra, are determined using a spectrophotometer. In order to obtain the photosynthetic pigment’s absorption spectra the pigments are separated using paper chromatography. Paper chromatography is an analytical technique that separates a mixture based on the individual pigment’s size, polarity and solubility (Lewis, 2004). The separation of the mixtures involves a stationary phase (the chromatography paper), which a mobile phase (solvent) moves up through. When the mixtures is applied to the paper and allowed to flow with the mobile phase, the different pigments move at different rates (Campbell, 1996). This means the pigments that absorb the strongest to the stationary phase (the chromatography paper) will move the slowest, while the weakest will move the fastest. The rate of the pigments movement will separate each pigment individually from the mixture (Maitland, 2002). This natural separation shows that each pigment is chemically different and plays different roles in photosynthesis (Maitland, 2002). To analyze the separated pigments a spectrophotometer is used to obtain an absorbance spectrum. This spectrum is a graph that shows a pigment’s light absorption versus wavelengths
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